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Polyfunctional chemically bonded stationary phase for reversed phase high-performance liquid chromatography

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Summary

Preparation of a chemically-bonded-silica stationary phase (SG-MIX), with different polar and nonpolar functional groups such as hydroxyl (-OH), amino (-NH2), cyano (-CN), phenyl (-Ph), octyl (-C8) and octadecyl (-C18), is described. The characteristics of the prepared packing was done by physicochemical methods such as elemental analysis, FTIR spectroscopy, solid-state nuclear magnetic resonance (NMR), chromatography (GC and HPLC) as well as by analysis of quantitative structure-retention relationships (QSRR). The polyfunctional SG-MIX phase possesses properties of a reversed-phase material modified by specific inputs to retention due to interactions of analytes with polar functionalities on the surface of adsorbent. The new type of packing was successfully used for both analytical and preparative separation of finasteride from its metabolite, N-methylfinasteride.

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Authors and Affiliations

  1. Department of Environmental Chemistry, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin Str., 87 100, Toruñ, Poland

    B. Buszewski & R. Gadzała-Kopciuch

  2. Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdañsk, Gen. J. Hallera 107, 80 416, Gdañsk, Poland

    R. Kaliszan & M. Markuszewski

  3. Department of Chemistry, University of San Jose, One Washington Square, 95192-0101, San Jose, CA, USA

    M. T. Matyska & J. J. Pesek

Authors
  1. B. Buszewski
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  2. R. Gadzała-Kopciuch
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  3. R. Kaliszan
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  4. M. Markuszewski
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  5. M. T. Matyska
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  6. J. J. Pesek
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Additional information

This work is dedicated to Prof. Dr. hab. Edward Soczewiñski on the occasion of his 70th birthday.

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Buszewski, B., Gadzała-Kopciuch, R., Kaliszan, R. et al. Polyfunctional chemically bonded stationary phase for reversed phase high-performance liquid chromatography. Chromatographia 48, 615–622 (1998). https://doi.org/10.1007/BF02467590

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  • DOI: https://doi.org/10.1007/BF02467590

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