Skip to main content
Log in

Biosurfactant production and use in oil tank clean-up

  • Technical Communication
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

A proprietary bacterial strain (Pet 1006) produced biosurfactants when grown on both glucose and an immiscible hydrocarbon as carbon sources. Pilot-plant-scale (1500 I) production gave, on repeated batch runs, 2 tonnes of culture broth containing active biosurfactant. The product was used as a substitute for chemical surfactants in a clean-up demonstration test carried out by Cargo Fleet Chemical Company Ltd. (UK) on an oil storage tank belonging to Kuwait Oil Company, Kuwait. The clean-up was successful in removing the sludge from the tank bottom, and it also allowed the recovery of more than 90% of the hydrocarbon trapped in the sludge. The recovered hydrocarbon had excellent properties and could be sold after being blended with fresh crude.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Cooper, D.G., MacDonald, C.R., Duff, S.J. &Kosaric, N. 1981 Enhanced production of surfactant fromBacillus subtilis by continuous product removal and metal cations additions.Applied and Environmental Microbiology 42, 408–412.

    CAS  Google Scholar 

  • Cundell, A.M. &Traxter, R.W. 1973 Microbial degradation of petroleum at low temperature.Marine Pollution Bulletin 4, 125–127.

    CAS  Google Scholar 

  • Desai, J.D. 1987 Microbial surfactants: evaluation, types, production and future application.Journal of Science and Industrial Research 46, 440–449.

    CAS  Google Scholar 

  • Guerra-Santos, L., Kappeli, O. &Fiechter, A. 1984Pseudomonas aeruginosa biosurfactant production in continuous culture with glucose as carbon source.Applied and Environmental Microbiology 48, 301–305.

    CAS  Google Scholar 

  • Guerra-Santos, L., Kappeli, O. &Fiechter, A. 1986 Dependence ofPseudomonas aeruginosa continuous culture biosurfactant production on nutritional and environmental factors.Applied Microbiology and Biotechnology 24, 443–448.

    Article  CAS  Google Scholar 

  • Gutnick, D.L. &Rosenberg, E. 1977 Oil tankers and pollution: a microbiological approach.Annual Review of Microbiology 31, 379–396.

    Article  CAS  Google Scholar 

  • Hisatsuka, K., Nakahara, T., Sang, N. &Yamada, K. 1971 Formation of rhamnolipid byPseudomonas aeruginosa and its function in hydrocarbon fermentation.Agricultural and Biological Chemistry 35, 686–692.

    Google Scholar 

  • Hitzman, D.O. 1983 Petroleum microbiology and the history of its role in enhanced oil recovery. InProceedings, 9982 International Conference on Microbial Enhancement of Oil Recovery, ed. Donaldson, E.C. & Clark, J.B., pp. 162–218, Bartlesville, OK: Bartlesville Energy Technology Center.

    Google Scholar 

  • Ito, S. &Inoue, S. 1982 Sophorolipids fromTorulopsis bombicola possible relation to alkane uptake.Applied and Environmental Microbiology 43, 1278–1283.

    CAS  Google Scholar 

  • Jobson, A., Cook, F.D. &Westlake, D.W.S. 1972 Microbial utilization of crude oil.Applied Microbiology 23, 1082–1089.

    CAS  Google Scholar 

  • Kappeli, O. &Finnerty, W.R. 1980 Characteristics of hexadecane partition by the growth medium ofAcinetobacter sp.Biotechnology and Bioengineering 22, 494–503.

    Google Scholar 

  • Kosaric, N., Gray, N.C.C. &Cairns, W.L. 1983 Microbial emulsifiers and de-emulsifiers. InBiotechnology, Vol. 3, ed. Rehm, H.J. & Reed, G., pp. 575–592. Weinheim: Verlag Chemie.

    Google Scholar 

  • Mulkilns-Philips, G.J. &Stewart, J.E. 1974 Effect of environmental parameters on bacterial degradation of Bunker C. oil, crude oils, and hydrocarbons.Applied Microbiology 28, 915–922.

    Google Scholar 

  • Person, A. &Molin, G. 1987 Capacity for biosurfactant production of environmentalPseudomonas andVibrionaceae growing on carbohydrate.Applied Microbiology and Biotechnology 26, 439–442.

    Google Scholar 

  • Prince, E.L. &Morton, L.H.G. 1989 Biofilms and bioemulsifiers in hydrocarbon degradation.International Biodeterioration 25, 385–392.

    Article  CAS  Google Scholar 

  • Rapp, P., Bock, H., Wray, V. &Wagner, F. 1979 Formation, isolation and characterization of trehalose dimycolates fromRhodococcus erythropolis grown onn-alkanes.Journal of General Microbiology 115, 491–503.

    CAS  Google Scholar 

  • Robert, M., Mercade, M.E., Bosch, M.P., Parra, J.L., Espuny, M.J., Manresa, M.A. &Guinea, J. 1989 Effect of the carbon source on biosurfactant production byPseudomonas aeruginosa.Biotechnology Letters 11, 871–874.

    Article  CAS  Google Scholar 

  • Rosenberg, E. 1986 Microbial surfactants.CRC Critical Reviews in Biotechnology 3, 109–132.

    CAS  Google Scholar 

  • Singer, M.E., Finnerty, W.R., Bolden, P. &King, A.D. 1983 Microbial processes in the recovery of heavy petroleum.Proceedings, 1982 International Conference on Microbial Enhancement of Oil Recovery, ed. Donaldson, E.C. & Clark, J.B. pp. 94–101. Bartlesville, OK: Bartlesville Energy Technology Center.

    Google Scholar 

  • Walker, J.D. &Colwell, R.R. 1974 Microbial petroleum degradation: use of mixed hydrocarbon substrates.Applied Microbiology 27, 1053–1060.

    CAS  Google Scholar 

Download references

Authors

Additional information

I.M. Banat is at 5, Upper Galliagh Road, Londonderry, Northern Ireland BT48 8LW, UK but was at the Kuwait Institute for Scientific Research at the time this paper was written. The remaining authors are with the Kuwait Institute for Scientific Research, Biotechnology Department, P.O. Box 24885, 13109, Safat, Kuwait. I.M. Banat is the corresponding author.

In view of the annexation of Kuwait by Iraq in August 1990, this paper has been accepted without return to the author for attention to minor details and for approval of certain editorial changes that have been made. The Editor-in-Chief therefore assumes full responsibility for any errors or omissions.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Banat, I.M., Samarah, N., Murad, M. et al. Biosurfactant production and use in oil tank clean-up. World J Microbiol Biotechnol 7, 80–88 (1991). https://doi.org/10.1007/BF02310921

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02310921

Keywords

Navigation