Summary
1. The absorption of intraduodenally administered carboxyl-14C glyceryl trioctanoate (RATO) was studied in 20 normal dogs, in 9 after pancreatectomy and in 4 after pancreatectomy and thoracic duct fistula.
2. The rate of absorption was estimated by continuously monitoring the expired14CO2 after RATO administration. In 20 control animals, an average of 26.7% ± 5.2% of the administered dose of radioactivity was recovered as14CO2 in 150 min. Following pancreatectomy,14CO2 recovery rates diminished significantly in all dogs.
3. The route of absorption was investigated by thin-layer and gas lipid chromatography of lymph collected from 4 of the pancreatectomized dogs after RATO and unlabeled trioctanoin administration. No 8-carbon fatty acids or glycerides were detected in the lymph by these techniques.
4. These results indicate that trioctanoin absorption is retarded in the absence of pancreatic lipase, but that fractional amounts of this lipid are absorbed via the portal route. Probable mechanisms for trioctanoin absorption in pancreatectomized dogs are discussed.
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References
Borgstrom, B. Transport form of14C- decanoic acid in portal and inferior vena cava blood during absorption in the rat.Acta Physiol Scand 34:71, 1955.
Borgstrom, B. “Metabolism of Glycerides.” InLipide Metabolism, Konrad Bloch, Ed. Wiley, New York, 1960, p. 27.
Cohen, B. J. Fat excretion in dogs lacking both bile and pancreatic juice.Proc Soc Exp Biol Med 107: 40, 1961.
Gelb, A. M., andKessler, J. I. Effect of fatty acid structure on esterification by the small intestine in vitro.Amer J Physiol 204:821, 1963.
Greenberger, N. J., Rodgers, J. B., andIsselbacher, K. J. Absorption of medium and long chain triglycerides: factors influencing their hydrolysis and transport.J Clin Invest 45:217, 1966.
Hashim, S. A., Bergen, S. S., Jr., Krell, K., andVan Itallie, T. B. Intestinal absorption and mode of transport in portal vein of medium chain fatty acids. (abst.).J Clin Invest 43:1238, 1964.
Kiyasu, J. Y., Bloom, B., andChaikoff, I. L. The portal transport of absorbed fatty acids.J Biol Chem 199:415, 1952.
Metcalfe, L. D., Schmitz, A. A., andPelka, J. R. Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis.Anal Chem 33: 363, 1961.
Peterson, M. L. On the re-esterification of fatty acids during absorption of fat: studies in patients with chyluria.Gastroenterology 44:774, 1963.
Playoust, M. R., andIsselbacher, K. J. Studies on the intestinal absorption and intramucosal lipolysis of a medium chain triglyceride.J Clin Invest 43:878, 1964.
Senior, J. R., andIsselbacher, K. J. Demonstration of an intestinal monoglyceride lipase: an enzyme with a possible role in the intracellular completion of fat digestion.J Clin Invest 42:187, 1963.
Tolbert, B. M., Kirk, M., andBaker, E. M. Continuous14CO2 excretion studies in experimental animals.Amer J Physiol 185:269, 1956.
Valdivieso, V. D., andSchwabe, A. D. The effect of exclusion of the hepatic circulation on the oxidation of octanoic acid in the rat.Proc Soc Exp Biol Med 116:290, 1964.
Valdivieso, V. D., andSchwabe, A. D. Factors influencing the absorption of a medium chain triglyceride. II. The role of pancreatic juice in the intraluminal phase of absorption.Gastroenterology 48:336, 1965.
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Supported in part by Grants AM-05957-03, AM-07961, and AM-07962, National Institute of Arthritis and Metabolic Diseases, U. S. Public Health Service, and by the John A. Hartford Foundation, Inc.
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Schwabe, A.D., Valdivieso, V.D., Merrill, S. et al. Studies on the intestinal absorption of a medium chain fat (Trioctanoin) in normal and pancreatectomized dogs. Digest Dis Sci 12, 1114–1121 (1967). https://doi.org/10.1007/BF02233877
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DOI: https://doi.org/10.1007/BF02233877