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The early mineralization of enamel

Fine structural observations on the cellular localization of calcium with the potassium pyroantimonate technique

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Summary

The potassium pyroantimonate technique was used to study the cellular distribution of calcium during the early mineralization of enamel in rat molar tooth germs at the electron microscope level. Differing patterns of calcium distribution were observed in the ameloblast seemingly associated with the appearance of Tomes' process. In the early secretory ameloblast calcium pyroantimonate deposits were observed within the Golgi apparatus, within coated vesicles, within mitochondria and associated with the inner aspect of the cell membrane. However, with the development of Tomes' process the ameloblasts no longer demonstrated these discrete deposits of calcium pyroantimonate. Instead they showed a diffuse cytoplasmic staining pattern with no preference for any particular organelle.

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References

  1. Boyd, A., Reith, E.J.: Qualitative electron probe analysis of secretory ameloblasts and odontoblasts in the rat incisor. Histochemistry50, 347–354 (1977)

    PubMed  Google Scholar 

  2. Bechtel, D.B., Horner, H.T.: Calcium excretion and deposition during sporogenesis in Physarella oblonga. Calcif. Tiss. Res.18, 195–213 (1975)

    Google Scholar 

  3. Beynon, A.D.: Enzymes in enamel maturation. Proc. Roy. Soc. Med.65, 911–912 (1972)

    PubMed  Google Scholar 

  4. Brighton, C.T., Hunt, R.M.: Mitochondrial calcium and its role in calcification. Histochemical localization of calcium in electron micrographs of the epiphyseal growth plate with K-pyroantimonate. Clin. Orthop. Rel. Res.100, 406–416 (1976)

    Google Scholar 

  5. Brighton, C.T., Hunt, R.M.: Histochemical localization of calcium in growth plate mitochondria and matrix vesicles. Fed. Proc.35, 143–147 (1976)

    PubMed  Google Scholar 

  6. Deporter, D.A., Ten Cate, A.R.: Fine structural localization of alkaline phosphatase in relation to enamel formation in the mouse molar. Archs. Oral Biol.21, 7–12 (1976)

    Google Scholar 

  7. Frank, R.M., Nalbandian, J.: Ultrastructure of amelogenesis. In: Structural and chemical organization of teeth (A.E.W. Miles, ed.), pp. 399–466. New York: Academic Press 1967

    Google Scholar 

  8. Glick, P.L., Edie, J.W.: Mineralization of rat enamel: a combined electron-microprobe, electron microscopic and his tologic study. Jour. Dent. Res. 54A, abstract 348, 1975

  9. Hohman, W., Schraer, H.: The intracellular distribution of calcium in the mucosa of the avian shell gland. J. Cell Biol.30, 317–331 (1966)

    PubMed  Google Scholar 

  10. Mathews, J.L., Martin, J.H., Arsenis, C., Eisenstein, R., Kuettner, K.: The role of mitochondria in intracellular calcium regulation. In: Cellular mechanisms for calcium transfer and homeostasis (G. Nichols and R.H. Wasserman, eds.), pp. 239–255. New York: Academic Press 1971

    Google Scholar 

  11. Munhoz, C.O.G., Leblond, C.P.: Deposition of calcium phosphate into dentine and enamel as shown by radiography of sections of incisor teeth following injection of45Ca into rats. Calcif. Tiss. Res.15, 221–235 (1974)

    Google Scholar 

  12. Nagai, N., Frank, R.M.: Tranfert du45Ca par autoradiographic en microscopie electronique au cours de l'amelogenese. Calcif. Tiss. Res.19, 211–221 (1975)

    Google Scholar 

  13. Nakahara, H., Bevelander, G.: An electron microscope and autoradiographic study of the calciferous glands of the earthworm, Lumbricus terrestris. Calcif. Tiss. Res.4, 193–201 (1969)

    Google Scholar 

  14. Reith, E.J.: The binding of calcium within the Golgi saccules of the rat odontoblast. Amer. J. Anat.147, 267–272 (1976)

    PubMed  Google Scholar 

  15. Reith, E.J., Cotty, V.F.: Autoradiographic studies on calcification of enamel. Archs. Oral Biol.7, 365–372 (1962)

    Google Scholar 

  16. Reith, E.J., Cotty, V.F.: The absorptive activity of ameloblasts during the maturation of enamel. Anat. Rec.157, 577–588 (1967).

    PubMed  Google Scholar 

  17. Shapiro, I.M., Greenspan, J.S.: Are mitochondria directly involved in biological mineralization? Calcif. Tiss. Res.3, 100–102 (1969)

    Google Scholar 

  18. Slavkin, H.C., Mino, W., Bringas, P.: The biosynthesis and secretion of precursor enamel protein by ameloblasts as visualized by autoradiography after tryptophan administration. Anat. Rec.185, 289–312 (1976)

    PubMed  Google Scholar 

  19. Spicer, S.S., Hardin, J.H., Green, W.B.: Nuclear precipitates in pyroantimonate-osmium tetroxide-fixed tissues. J. Cell Biol.39, 216–221 (1968)

    PubMed  Google Scholar 

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  1. Faculty of Dentistry, University of Toronto, 124 Edward Street, M5G 1G6, Toronto, Ontario, Canada

    D. A. Deporter

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  1. D. A. Deporter
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Deporter, D.A. The early mineralization of enamel. Calc. Tis Res. 24, 271–274 (1977). https://doi.org/10.1007/BF02223327

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  • DOI: https://doi.org/10.1007/BF02223327

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