Abstract
We have isolated a mutant ofSchizosaccharomyces pombe whose growth is temperature sensitive when it is haploid but not when it is diploid. This mutant may provide a useful system for selecting nonconditional mutants which are defective in diploid formation upon conjugation.
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Alfa C, Fantes P, Hyams J, Mcleod M, Warbrick E (1993) Experiments with fission yeast: a laboratory course manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
de Barros Lopes M, Ho J-Y, Reed SI (1990) Mutations in cell division cycle genesCDC36 andCDC39 activate the Saccharomyces cerevisiae mating pheromone response pathway. Mol Cell Biol 10:2966–2972
Chikashige Y, Ding D-Q, Funabiki H, Haraguchi T, Mashiko S, Yanagida M, Hiraoka Y (1994) Telomere-led premeiotic chromosome movement in fission yeast. Science 264:270–273
Costello G, Rodgers L, Beach D (1986) Fission yeast enters the stationary phase G0 from either mitotic G1 or G2. Curr Genet 11:119–125
Dietzel C, Kurjan J (1987) The yeast SCG1 gene: a Gα-like protein implicated in thea- and α-factor response pathway. Cell 50:1001–1010
Funabiki H, Hagan I, Uzawa S, Yanagida M (1993) Cell cyledependent positioning and clustering of centromeres and telomeres in fission yeast. J Cell Biol 121:961–976
Goebl MG, Petes TD (1986) Most of the yeast genomic sequences are not essential for cell growth and division. Cell 46:983–992
Gutz H, Heslot H, Leupold U, Loprieno N (1974) Schizosaccharomyces pombe. In: King (ed) Handbook of genetics. Plenum Press, New York, pp 395–446
Kaback DB, Oeller PW, Steensma HY, Hirschman J, Ruezinsky D, Coleman KG, Pringle JR (1984) Temperature-sensitive lethal mutations in yeast chromosome 1 appear to define only a small number of genes. Genetics 108:67–90
Miyajima I, Nakafuku M, Nakayama N, Brenner C, Miyajima A, Kaibuchi K, Arai K-I, Kaziro Y, Matsumoto K (1987) GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction. Cell 50:1011–1019
Moreno S, Klar A, Nurse P (1991) Molecular genetic analysis of fission yeastSchizosaccharomyces pombe. Methods Enzymol 194:795–823
Niwa O, Yanagida M (1985) Triploid meiosis and aneuploidy inSchizosaccharomyces pombe: an unstable aneuploid disomic for chromosome III. Curr Genet 9:463–470
Scherthan H, Bahler J, Kohli J (1994) Dynamics of chromosome organization and pairing during meiotic prophase in fission yeast. J Cell Biol 127:273–285
Sherman F, Fink GR, Hicks JB (1986) Laboratory course manual for methods in yeast genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Uemura T, Yanagida M (1984) Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization. EMBO J 3:1737–1744
Weiner BM, Kleckner N (1994) Chromosome pairing via multiple interstitial interactions before and during meiosis in yeast. Cell 77:977–991
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Tange, Y., Niwa, O. A selection system for diploid and against haploid cells inSchizosaccharomyces pombe . Molec. Gen. Genet. 248, 644–648 (1995). https://doi.org/10.1007/BF02191703
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DOI: https://doi.org/10.1007/BF02191703