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The immunocytochemical localization of new soluble placental tissue proteins (PP14, 16, 17, 19, 20 and PP21) in human andCynomolgus monkey placentae

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Summary

Apparently Placenta-specific placental tissue proteins (PP14 and PP17) and solitary tissue proteins (PP16, 19, 20 and PP21) were investigated by avidin-biotin immunoperoxidase technique in the human andcynomolgus monkey placentae, membranes, decidua and umbilical cords. In human early placentae, PP14, 16, 17, 19 and PP21 were localized mainly in the cytoplasm of villous syncytiotrophoblast. PP20 was localized in the cytoplasm of basal chorionic trophoblasts. In human term placentae, positive stainings for PP16, 19 and PP21 were observed mainly in all kinds of trophoblastic cells, while positive stainings for PP14, 17 and PP20 were weakened in the trophoblastic cells. PP20 was clearly localized in the cytoplasm of Hofbauer-like cells in the villous stroma. The membrane of villous syncytiotrophoblast showed strongly positive stainings for PP21. PP21 was also localized in the membrane of amniotic and umbilical epithelium. The umbilical epithelium was cytoplasmically positive for PP14, 16 and PP20. Clear positive stainings for PP14 and PP21 were found in the cytoplasm of fetal polymorphonuclear neutrophils. All of the placental proteins were immunocytochemically positive in the decidual large cells. In thecynomolgus monkey placentae, similar immunostaining results were obtained. The monkey could, thus, serve as a model for the investigation of the placental proteins.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Faculty of Medicine, Chiba University, Inohana 1-8-1, Chiba, 280, Chiba, Japan

    Noriyuki Inaba, Naomi Sato, Ichio Fukazawa, Yoriko Ota, Shouichi Shirotake & Hiroyoshi Takamizawa

  2. Department of Obstetrics and Gynecology, Faculty of Medicine, Keio University, Shinanomachi 35, Shinjukuku, 160, Tokyo, Japan

    Shirou Nozawa

  3. Research Laboratories, Behringwerk AG, D-3550, Marburg/Lahn, Federal Republic of Germany

    Hans Bohn

Authors
  1. Noriyuki Inaba
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  2. Naomi Sato
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  3. Ichio Fukazawa
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  4. Yoriko Ota
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  5. Shouichi Shirotake
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  6. Hiroyoshi Takamizawa
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  7. Shirou Nozawa
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  8. Hans Bohn
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Inaba, N., Sato, N., Fukazawa, I. et al. The immunocytochemical localization of new soluble placental tissue proteins (PP14, 16, 17, 19, 20 and PP21) in human andCynomolgus monkey placentae. Arch. Gynecol. 240, 13–19 (1987). https://doi.org/10.1007/BF02134059

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  • DOI: https://doi.org/10.1007/BF02134059

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