Abstract
HLA-DRB1 allele typing was performed by the PCR-RFLP method on 59 ulcerative colitis (UC) patients and 136 healthy controls. Phenotypic frequencies of HLA-B52 and DR2 were significantly increased among the UC patients, serologically. DNA typing of HLA-DRB1 revealed that the genotypic frequency of DRB1*1502 was higher in UC than in the controls (49.2% vs 17.6%;P<0.0001). In the analysis of clinical parameters, 82.8% of patients bearing DRB1*1502 were treated with corticosteroids. DRB1*1501 and DRB1*1502 differ in only one amino acid at residue 86 (valine vs glycine), and 66% of the UC patients carried two glycines at position 86 in the HLA-DRβ-chain (vs 51% of control;P<0.05). These observations suggest that the presence of Gly-86 in the HLAβ-chain and surrounding amino acid sequence of HLA-DRB1*1502 is strongly associated with susceptibility to UC.
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Asakura H, Tsuchiya M, Aiso S, Watanabe M, Kobayashi K, Hibi T, Ando K, Tabata H, Sekiguch S: Association of the human lymphocyte-DR2 antigen with Japanese ulcerative colitis. Gastroenterology 82:413–418, 1982
Sugimura K, Asakura H, Mizuki N, Inoue M, Hibi T, Yagita A, Tsuji K, Inoko H: Analysis of genes within the HLA region affecting susceptibility to ulcerative colitis. Hum Immunol 36:112–118, 1993
Delpre G, Kadish U, Gazit E, Joshua H, Zamir R: HLA antigens in ulcerative colitis and Crohns disease in Israel. Gastroenterology 78:1452–1457, 1980
Ota M, Seki T, Tsuji T, Inoko H: HLA-DRB1 genotyping by modified PCR-RFLP method combined with group-specific primers. Tissue Antigen 39:187–202, 1992
Verreck FA, Terjmijtelen A, Koning F: HLA-DRβ chain residue 86 controls DRαβ dimer stability. Eur J Immunol 23:1346–1350, 1993
Demotz S, Barbey C, Corradin G, Amoroso A, Lanzavecchia A: The set of naturally processed peptides displayed by DR molecules is tuned by polymorphism of residue 86. Eur J Immunol 23:425–432, 1993
Toyoda H, Wang SJ, Yang HY, Redford A, Magalong D, Tyan D, Mcrlree CK, Pressman SR, Shanahan F, Targan SR, Rotter JI: Distinct associations of HLA class II genes with inflammatory bowel disease. Gastroenterology 104:741–748, 1993
Brown JH, Jardetzky TS, Gorga JC, Stern LJ, Urban RG, Strominger JL, Wiley DC: Three-dimensional structure of the human class II histocompatibility antigen HLA-DR1. Nature 364:33–39, 1993
Newton-Nash DK, Eckels DD: Differential effect of polymorphism at HLA-DR1β-chain positions 85 and 86 on binding and recognition of DR1-restricted antigenic peptides. J Immunol 150:1813–1821, 1993
Todd JA, Bell JI, McDevitt HO: HLA-DQβ gene contributes to susceptibility and resistance to insulin-dependent diabetes mellitus. Nature 329:599–604, 1987
Erlich HA, Zeidler A, Chang J, Shaw S, Raffel LJ, Klitz W, Beshkov Y, Costin G, Pressman S, Bugawan T, Rotter JI: HLA class II alleles and susceptibility and resistance to insulin dependent diabetes mellitus in Mexican-American families. Nature Genet 3:358–364, 1993
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Futami, S., Aoyama, N., Honsako, Y. et al. HLA-DRB1*1502 allele, subtype of DR15, is associated with susceptibility to ulcerative colitis and its progression. Digest Dis Sci 40, 814–818 (1995). https://doi.org/10.1007/BF02064985
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DOI: https://doi.org/10.1007/BF02064985