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Immunohistological localization of testosterone in the growing antler of the white-tailed deer (Odocoileus virginianus)

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Abstract

Using immunohistological methods (fluorescein and peroxidase labeled double antibody technique), the localization of testosterone in the antlers of white-tailed deer (Odocoileus virginianus) at the beginning of the period of accelerated calcification was examined. The most intense reaction for testosterone was found in the prochondral blastema layer and in the growing follicles of the velvet. The findings may indicate the importance of testosterone in bone matrix synthesis.

Résumé

La localisation de testostérone dans les andouillers de daims à queue blanche (Odocoileus virginianus), au début de la période de calcification accélérée, est étudiée par des méthodes immunohistologiques (la technique d'anticorps doublement marqués à la fluorescéine et la péroxydase). La réaction la plus intense à la testostérone est observée dans la couche du blastème prochondrale et dans les follicules en voie de développement de la partie veloutée des andouillers. Ces résultats semblent confirmer l'importance de la testostérone dans la synthèse de la matrice osseuse.

Zusammenfassung

Die Lokalisation von Testosteron im Geweihgewebe des Weißwedelhirsches (Odocoileus virginianus) zu Beginn der Phase der beschleunigten Kalzifizierung wurde unter Anwendung der doppelten Antikörper-Technik (Fluorescein- und Peroxidase-Markierung) immunohistologisch untersucht. Die größte Anhäufung von Testosteron wurde in den Schichten des prochondralen Blastems und in den wachsenden Follikeln des Bastgewebes beobachtet. Die Befunde weisen auf die Bedeutung des Testosterons für die Matrix-Synthese hin

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References

  1. Adachi, A., Uno, H.: Glucose metabolism of growing and resting human hair follicles. Amer. J. Physiol.215, 1234–1239 (1968)

    PubMed  Google Scholar 

  2. Belanger, L. F., Choquette, L. P. E., Cousineau, A.: Osteolysis in reindeer antlers; sexual seasonal variations. Calcif. Tissue Res.1, 37–43 (1967)

    PubMed  Google Scholar 

  3. Bramley, P. S.: Territoriality and reproductive behaviour of roe deer. J. Reprod. Fertil., Suppl.11, 43–70 (1970)

    Google Scholar 

  4. Bubenik, A. B.: Das Geweih. Hamburg: Paul Parey 1966

    Google Scholar 

  5. Bubenik, G. A.: Seasonal variations of nuclear size of hypothalamic cells in the roe-buck. J. Anim. Sci.35, 967–973 (1972)

    PubMed  Google Scholar 

  6. Bubenik, G. A., Bubenik, A. B.: Testosterone blockade in the antler growth process. In preparation (1973)

  7. Bubenik, G. A., Brown, G. M., Grota, L. J.: Immunohistochemical localization of testosterone in testicular tissue. Anat. Rec.175, 280 (1973) (Abstract)

    Google Scholar 

  8. Erlanger, B. F., Beiser, S. M., Borek, F., Edel, F., Lieberman, S.: The preparation of steroid-protein conjugates to elicit antihormonal antibodies. In: Methods of immunology and immunohistochemistry (Williams, C. A., Chase, M. C., eds.), vol. 1, p. 144, New York: Academic Press 1967

    Google Scholar 

  9. Frankenberger, Z.: Interstitial cells of red deer (Cervus elaphus). Čs. Morfol.2, 36–41 (1954)

    Google Scholar 

  10. Frankenberger, Z.: Some remarks on the mechanisms of the shedding of the antlers in the deer. Čs. Morfol.9, 41–45 (1961)

    Google Scholar 

  11. Goss, R. J.: The deciduous nature of deer antlers. In: Mechanism of hard tissue destruction, Pupl. 75, p. 339–369. Washington, D. C.: Amer. Ass. for Advancement of Sci. 1963

    Google Scholar 

  12. Goss, R. J.: Inhibition of growth and shedding of antlers by sex hormones. Nature (Lond.)220, 83–85 (1968)

    Google Scholar 

  13. Goss, R. J.: Principles of regenerations, p. 223–225. New York: Academic Press 1969

    Google Scholar 

  14. Goss, R. J.: Problem of antlerogenesis. Ortopaedics69, 227–238 (1970)

    Google Scholar 

  15. Hartman, B. K., Udenfried, S.: The application of immunological techniques to the study of enzymes regulating catecholamine synthesis and degradation. Pharmacol. Rev.24, 311–330 (1972)

    PubMed  Google Scholar 

  16. Hartwig, H.: Experimentelle Untersuchungen zur Entwicklungsphysiologie der Stangenbildung beim Reh (Capreolus c. capreolus. L. 1758). Wilhelm Roux' Arch. Entwickl.-Mech. Org.158, 358–384 (1967)

    Google Scholar 

  17. Hertel, P., Kramer, M., Neumann, F.: Einfluß eines Antiandrogens (Cyproteroneacetat) auf Knochenwachstum and Knochenreifung männlicher Ratten. Arzneimittel-Forsch.19, 1777–1790 (1969)

    Google Scholar 

  18. Kirschner, M. A., Jacobs, J. B.: Combined ovarian and adrenal vein catheterization to determine the site(s) of androgen overproduction in hirsute women. J. clin. Endocr.33, 199–209 (1971)

    PubMed  Google Scholar 

  19. Kuhlmann, R. B., Rainey, R., O'Neill, R.: Biochemical investigations of deer antler growth. J. Bone J. Surg.45, 345–350 (1963)

    Google Scholar 

  20. Laschet, U., Laschet, L.: Einfluß von Cyproteron acetat auf das neuroendokrine System des Menschen. Schering Symp. Über Sexualdeviation und ihre medikamentöse Behandlung, S. 89–98 (1972)

  21. Lojda, Z.: Histogenesis of the antlers of our Cervidae and its histological picture [in Czech]. Čs. Morfol.4, 43–65 (1956)

    Google Scholar 

  22. Markwald, R. R., Davis, R. W., Kainer, R. A.: Histological and histochemical periodicity of Leydig cells in relation to antler growth. Gen. comp. Endocr.16, 268–280 (1971)

    PubMed  Google Scholar 

  23. Mitjushev, P. V.: Farming and diseases of deer kept for harvesting of growing antler [in Russian], p. 240. Moskva: Mezhd. Kniga. 1950

    Google Scholar 

  24. Mohr, E.: Über Geweih und Geweihzyklus beim Milu, Elaphurus davidianus M.-Edw. Wiss. u. Kult. Mitt. aus dem Tierpark Berlin1, 156–163 (1962)

    Google Scholar 

  25. Nakane, P. K., Pierce, G. B.: Enzyme-labeled antibodies: preparation and application for the localization of antigens. J. Histochem. Cytochem.14, 929–931 (1966)

    Google Scholar 

  26. Olt, A.: Die Perücke der Cerviden und das Karzinom. Dtsch. tierärztl. Wschr.35, 131–133 (1927)

    Google Scholar 

  27. Ries, J.: Das Hirschgeweih als Aufspeicherungsort für Sexualhormone. Schweiz. med. Prax.37, 368–379 (1948)

    Google Scholar 

  28. Short, R. V., Mann, T.: The sexual cycle of seasonally breeding mammal, the roe-buck, Capr. capreolus. J. Reprod. Fertil.12, 337–351 (1966)

    PubMed  Google Scholar 

  29. Simpson, G. G.: The principles of classification and a classification of mammals. Bull. of the Amer. Museum of National History85, 1–350 (1945)

    Google Scholar 

  30. Steczek, K., Vertes, K., Koref, O., Feher, T.: Bestimmung der Östrogene im Harn von Männern und bei Frauen außerhalb der Schwangerschaft. Endokrinologie53, 72–77 (1968)

    PubMed  Google Scholar 

  31. Stieve, R.: Anatomische Untersuchungen über die Fortpflanzungstätigkeit des europäischen Rehes, Capr. capreolus capr. Z. mikr. Anat.44, 427–530 (1950)

    Google Scholar 

  32. Stumpf, E. E., Baerwaldt, C., Sar, M.: Autoradiographic cellular and subcellular localization of sexual steroids. In: Basic action of sex steroids on target organs (Hubinont, P. O., Leroy, F., Galand, P., eds.) p. 3–20, Basel: Karger 1971

    Google Scholar 

  33. Tachezy, R.: Über den Einfluß der Sexualhormone auf das Geweihwachstum der Cerviden. Säugetierkundl. Mitt.4, 103–112 (1956)

    Google Scholar 

  34. Takayasu, S., Adachi, K.: The conversion of testosterone to 17-Hydroxy-5-androstan-3-one-Dihydrotesterone-by human hair follicles. J. clin. Endocr.34, 1098–1101 (1972)

    PubMed  Google Scholar 

  35. Thompson, R. G., Rodriguez, A., Kowarski, A., Migeon, C. J., Blizzard, R. M.: Integrated concentration of growth hormone correlated with plasma testosterone and bone age in preadolescent and adolescent males. J. clin. Endocr.35, 334–337 (1972)

    PubMed  Google Scholar 

  36. Waldo, C. M., Wislocki, G. B.: Observations on the shedding of antlers of Virginia deer (Odocoileus virginianus borealis). Amer. J. Anat.88, 351–395 (1951)

    PubMed  Google Scholar 

  37. Wislocki, G. B., Aub, J. C., Waldo, C. M.: The effects of gonadectomy and the administration of testosterone propionate on the growth of antlers in male and female deer. Endocrinology40, 202–224 (1947)

    Google Scholar 

  38. Wislocki, G. B., Waldo, C. M.: Further observations on the histological changes associated with the shedding of the antlers of the white-tailed deer (Odocoileus virginianus borelais). Anat. Rec.117, 353–375 (1953)

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Neuroendocrinology Research Section, Clarke Institute of Psychiatry, 250 College St., M5T 1R8, Toronto, Ontario, Canada

    G. A. Bubenik & G. M. Brown

  2. Fish & Wildlife Research Branch, Ontario Ministry of Natural Resources, Maple, Ontario

    A. B. Bubenik

  3. Department of Psychiatry, University of Rochester, Rochester, New York

    L. J. Grota

Authors
  1. G. A. Bubenik
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  2. G. M. Brown
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  3. A. B. Bubenik
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  4. L. J. Grota
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Additional information

The project was funded by the Swiss Foundation for Alpine Research, Zurich, Switzerland and Ontario Mental Health Foundation, Canada.

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Bubenik, G.A., Brown, G.M., Bubenik, A.B. et al. Immunohistological localization of testosterone in the growing antler of the white-tailed deer (Odocoileus virginianus). Calc. Tis Res. 14, 121–130 (1974). https://doi.org/10.1007/BF02060288

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  • DOI: https://doi.org/10.1007/BF02060288

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