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Prophylaxis ofPseudomonas aeruginosa infections in leukopenic mice by a combination of active and passive immunization

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Abstract

Mice rendered leukopenic with cyclophosphamide and then challenged with viablePseudomonas aeruginosa were used to determine the protective efficacy of active immunization against exotoxin A and of passive immunization with human antiserum toEscherichia coli J5, a rough mutant ofEscherichia coli O111∶B4. Neither treatment alone provided a greater degree of protection than its respective control. However, the combination of these treatments produced a moderate, yet consistent, increase in the survival of infected immunosuppressed mice.

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References

  1. Pruitt, B. A., Jr., Lindberg, R. B.:Pseudomonas aeruginosa infections in burn patients. In: Doggett, R. G. (ed.):Pseudomonas aeruginosa: Clinical manifestations of infection and current therapy. Academic Press, New York, 1979, p. 339–366.

    Google Scholar 

  2. Rodriguez, V., Bodey, G. P.: Epidemiology, clinical manifestations, and treatment in cancer patients. In: Doggett, R. G. (ed.):Pseudomonas aeruginosa: Clinical manifestations of infection and current therapy. Academic Press, New York, 1979, p. 368–407.

    Google Scholar 

  3. Young, L. S.: Recent advances in the therapy ofPseudomonas aeruginosa infection. In: Doggett, R. G. (ed.):Pseudomonas aeruginosa: Clinical manifestations of infection and current therapy. Academic Press, New York, 1979, p. 311–338.

    Google Scholar 

  4. Pennington, J. E.: Immunotherapy ofPseudomonas aeruginosa infection. In: Doggett, R. G. (ed.):Pseudomonas aeruginosa: Clinical manifestations of infection and current therapy. Academic Press, New York, 1979, p. 192–217.

    Google Scholar 

  5. Chedid, L., Parent, M., Boyer, F.: A proposed mechanism for natural immunity to enterobacterial pathogens. Journal of Immunology 1968, 100: 293–301.

    Google Scholar 

  6. McCabe, W. R., DeMaria, A., Johns, M.: Potential use of shared antigens for immunization against gramnegative bacillary infections. In: Mizrahi, A., Hertman, I., Klingberg, M. A., Kohn, A.: New developments with human and veterinary vaccines. Progress in clinical and biological research. Volume 47. Alan R. Liss, Inc., New York, 1980, p. 107–117.

    Google Scholar 

  7. Ziegler, E. J., McCutchan, J. A., Braude, A. I.: Treatment of gram-negative bacteremia with antiserum to core glycolipid. I. The experimental basis of immunity to endotoxin. European Journal of Cancer 1979, 15: 71–76.

    PubMed  Google Scholar 

  8. Braude, A. I., Douglas, H.: Passive immunization against the local Shwartzman reaction. Journal of Immunology 1972, 108: 505–512.

    CAS  Google Scholar 

  9. Greisman, S. E., DuBuy, J. B., Woodward, C. L.: Experimental gram-negative bacterial sepsis: prevention of mortality not preventable by antibiotics alone. Infection and Immunuty 1979, 25: 538–557.

    CAS  Google Scholar 

  10. Helting, T. B., Grewal, K. K., Oberwalder, U., Hammerschmid, F., Jaksche, H.: Prospects for the development of a hyperimmunoglobulin with activity against gramnegative organisms. In: Nydegger, U. E. (ed.): Immunotherapy: a guide to immunoglobulin prophylaxis and therapy. Academic Press, New York, 1981, p. 141–150.

    Google Scholar 

  11. Johns, M., Skehill, A., McCabe, W. R.: Immunization with rough mutants ofSalmonella minnesota. IV. Protection by antisera to O and rough antigens against endotoxin. Journal of Infectious Diseases 1983, 147: 57–67.

    PubMed  CAS  Google Scholar 

  12. Pennington, J. E., Menkes, E.: Type-specific vs crossprotective vaccination for gram-negative bacterial pneumonia. Journal of Infectious Diseases 1981, 144: 599–603.

    PubMed  CAS  Google Scholar 

  13. Peter, G., Chernow, M., Keating, M. H., Ryff, J. C., Zinner, S. H.: Limited protective effect of rough mutant antisera in murineEscherichia coli bacteremia. Infection 1982, 10: 228–232.

    Article  PubMed  CAS  Google Scholar 

  14. Ziegler, E. J., Douglas, H., Sherman, J. E., Davis, C. E., Braude, A. I.: Treatment ofEscherichia coli andKlebsiella bacteremia in agranulocytic animals with antiserum to a UDP-Gal epimerase-deficient mutant. Journal of Immunology 1973, 111: 433–438.

    CAS  Google Scholar 

  15. McCutchan, J. A., Ziegler, E. J., Braude, A. I.: Treatment of gram-negative bacteremia with antiserum to core glycolipid II. A controlled trial of antiserum in patients with bacteremia. European Journal of Cancer 1979, 15: 77–80.

    PubMed  Google Scholar 

  16. Ziegler, E. J., McCutchan, J. A., Fierer, J., Glauser, M. P., Sadoff, J. C., Douglas, H., Braude, A. I.: Treatment of gram-negative bacteremia and shock with human antiserum to a mutantEscherichia coli. New England Journal of Medicine 1982, 307: 1225–1230.

    Article  PubMed  CAS  Google Scholar 

  17. Liu, P. V.: Toxins ofPseudomonas aeruginosa. In: Doggett, R. G. (ed.):Pseudomonas aeruginosa: Clinical manifestations of infection and current therapy. Academic Press, New York, 1979, p. 63–88.

    Google Scholar 

  18. Cross, A. S., Sadoff, J. C., Iglewski, B. H., Sokol, P. A.: Evidence for the role of toxin A in the pathogenesis of infection withPseudomonas aeruginosa in humans. Journal of Infectious Diseases 1980, 142: 538–546.

    PubMed  CAS  Google Scholar 

  19. Pollack, M., Young, L. S.: Protective activity of antibodies to exotoxin-A and lipopolysaccharide at the onset ofPseudomonas aeruginosa septicemia in man. Journal of Clinical Investigation 1979, 63: 276–286.

    Article  PubMed  CAS  Google Scholar 

  20. Cryz, S. J., Jr., Pavlovskis, O. R., Iglewski, B. H.: Chemical and genetic approaches to makingPseudomonas aeruginosa toxin A toxoid. In: Robbins, J. B., Hill, J. C., Sadoff, J. C.: Seminars in infectious disease. Volume 4. Thieme-Stratton, Inc., New York, 1982, p. 70–79.

    Google Scholar 

  21. Pollack, M.:Pseudomonas toxin and toxoid. In: Robbins, J. B., Hill, J. C., Sadoff, J. C.: Seminars in infectious diseases. Volume 4. Thieme-Stratton, Inc., New York, 1982, p. 64–69.

    Google Scholar 

  22. Callahan, L. T., III, Martinez, D., Marburg, S., Tolman, R. L., Galloway, D. R.: Toxoids ofPseudomonas eruginosa exotoxin-A: photoaffinity inactivation of purified toxin and purified toxin derivatives. Infection and Immunity 1984, 43: 1019–1026.

    PubMed  CAS  Google Scholar 

  23. Lydick, E., McLean, A. A., Woodhour, A. F., Callahan, L. T., III: Responses of adult volunteers to aPseudomonas aeruginosa exotoxoid-A vaccine. Journal of Infectious Diseases 1985, 151: 375.

    PubMed  CAS  Google Scholar 

  24. Cryz, S. J., Jr., Furer, E., Germanier, R.: Protection againstPseudomonas aeruginosa infection in a murine burn wound sepsis model by passive transfer of antitoxin A, antielastase, and antilipopolysaccharide. Infection and Immunity 1983, 39: 1072–1079.

    PubMed  CAS  Google Scholar 

  25. Cryz, S. J., Jr., Furer, E., Germanier, R.: Passive protection againstPseudomonas aeruginosa infection in an experimental leukopenic mouse model. Infection and Immunity 1983, 40: 659–664.

    PubMed  Google Scholar 

  26. Pavlovskis, O. R., Edman, D. C., Leppla, S. H., Wretlind, B., Lewis, L. R., Martin, K. E.: Protection against experimentalPseudomonas aeruginosa infection in mice by active immunization with exotoxin A toxoids. Infection and Immunity 1981, 32: 681–689.

    PubMed  CAS  Google Scholar 

  27. Pavlovskis, O. R., Pollack, M., Callahan, L. T., III, Iglewski, B. A.: Passive protection by antitoxin in experimentalPseudomonas aeruginosa burn infections. Infection and Immunity 1977, 18: 596–602.

    PubMed  CAS  Google Scholar 

  28. Snell, K., Holder, I. A., Leppla, S. H., Saelinger, C. B.: Role of exotoxin and protease as possible virulence factors in experimental infections withPseudomonas aeruginosa. Infection and Immunity 1978, 19: 839–845.

    PubMed  CAS  Google Scholar 

  29. Ziegler, E. J., McCutchan, J. A., Douglas, H., Braude, A. I.: Prevention of lethalPseudomonas bacteremia with epimerase-deficientEscherichia coli antiserum. Transactions of the Association of American Physicians 1975, 88: 101.

    PubMed  CAS  Google Scholar 

  30. Pollack, M.: Antibody activity againstPseudomonas aeruginosa in immune globulins prepared for intravenous use in humans. Journal of Infectious Diseases 1983, 147: 1090–1098.

    PubMed  CAS  Google Scholar 

  31. Skarnes, R. C.: Host defense against bacterial endotoxemia: mechanism in normal animals. Journal of Experimental Medicine 1978, 132: 300–316.

    Google Scholar 

  32. Mumford, R. S., Hall, C. L., Dietschy, J. M.: Binding ofSalmonella typhimurium lipopolysaccharides to rat high-density lipoproteins. Infection and Immunity 1981, 34: 835–843.

    PubMed  Google Scholar 

  33. Ziegler, E. J., Douglas, H.:Pseudomonas aeruginosa vasculitis and bacteremia following conjunctivitis: a simple model of fatalPseudomonas infection in neutropenia. Journal of Infectious Diseases 1979, 139: 288–296.

    PubMed  CAS  Google Scholar 

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  1. Department of Virus and Cell Biology Research, Merck Sharp & Dohme Research Laboratories, 19486, West Point, Pennsylvania, USA

    D. Martinez & L. T. Callahan III

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  1. D. Martinez
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  2. L. T. Callahan III
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Martinez, D., Callahan, L.T. Prophylaxis ofPseudomonas aeruginosa infections in leukopenic mice by a combination of active and passive immunization. Eur. J, Clin. Microbiol. 4, 186–189 (1985). https://doi.org/10.1007/BF02013595

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