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Effects of butyrate and insulin and their interaction on the DNA synthesis of rumen epithelial cells in culture

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Summary

Rumen epithelial cells (REC) were incubated in the presence of various concentrations of butyrate or insulin or with both of them, to obtain information on their effect on the DNA synthesis of cultured cells. The 24-h values of3H-thymidine incorporation into cellular DNA were measured in the presence of butyrate, insulin or butyrate plus insulin. While butyrate reduced DNA synthesis, insulin produced an increase over the control. Combined butyrate plus insulin treatment influenced the incorporation of label in accordance with the relative proportion of these two substances.

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Authors and Affiliations

  1. Department of Physiology, University of Veterinary Sciences, P.O. Box 2, H-1400, Budapest, Hungary

    S. Neogrády, P. Gálfi & F. Kutas

Authors
  1. S. Neogrády
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  2. P. Gálfi
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  3. F. Kutas
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Neogrády, S., Gálfi, P. & Kutas, F. Effects of butyrate and insulin and their interaction on the DNA synthesis of rumen epithelial cells in culture. Experientia 45, 94–96 (1989). https://doi.org/10.1007/BF01990459

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  • DOI: https://doi.org/10.1007/BF01990459

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