Summary
An environmental strain ofBacillus subtilis was cultivated in a membrane bioreactor. Microbial cells were trapped by an upright membrane module fitted in the vessel reactor. Cell biomass and pectate lyase activity were increased about 5–6 times in comparison to a batch process. Clogging of the membrane module appears to be a major problem.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Charney J, Tomarelli RM (1947) A colorimetric method for the determination of the proteolytic activity of duodenal juice. J Biol Chem 177:501–505
Chemap (1983) European patent no. 0086539 A1:18p.
Cheryan M, Mehaia MA (1983) A high-performance membrane bioreactor for continuous fermentation of lactose to ethanol. Biotechnol Lett 5:519–524
Cheryan M, Mehaia MA (1985) Membrane bioreactors for high-performance fermentations. Am Chem Soc 281:231–245
Frankena J, Koningstein GM, Van Verseveld HW, Stouthamer AH (1986) Effect of different limitations in chemostat cultures on growth and production of extracellular protease byBacillus lichenoformis. Appl Microbiol Biotechnol 24:106–112
Goma G, Durand G (1986) Nouvelles conceptions de mise en oeuvre des microorganismes et de bioreacteurs basées sur les cultures à forte densité cellulaire CR 11th Coll Soc Fr Microbiol Toulouse 127–134
Jauneau A (1985) Contribution à la connaissance de la microflore pectinolytique: application au rouissage du lin. Thesis (3rd cycle) University of Rouen (France)
Jauneau A, Morvan O, Morvan C, Demarty M, Devauchelle G (1986) Mise en évidence d'une activité pectine-lyase chezBacillus subtilis. CR Acad Sc Paris 302, 3, 17: 641–646
Konno H, Yamasaki Y (1982) Studies on the pectic substances of plant cell walls. Degradation of carrot root cell walls by endopectate lyase purified fromErwinia aroideae. Plant Physiol 69:864–868
Kurowsky WM, Dunleavy JA (1976) Cellular and environmental factors affecting the synthesis of polygalacturonate lyase byBacillus subtilis. Eur J Appl Microbiol 2:103–112
Margaritis A, Wilke CR (1978) The rotofermentor. Description of the apparatus, power requirements, and mass transfer characteristics. Biotechnol Bioeng 20:709–727
Morvan O, Jauneau A, Morvan C, Demarty M, Ripoll C (1988) Degradation of pectic substances in green flax fibres byErwinia carotovora. Ann Appl Biol 112:107–116
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jauneau, A., Morvan, C., Fenyo, J.C. et al. Pectate lyase production byBacillus subtilis in a membrane bioreactor. Appl Microbiol Biotechnol 29, 234–238 (1988). https://doi.org/10.1007/BF01982908
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01982908