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Specificity of new peptide immunoassays versus other immunoassays and agglutination tests for detection of HIV antibody in African Sera

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Abstract

The specificity of three HIV EIAs using synthetic peptides was evaluated and compared with that of four commercial EIAs using recombinant proteins or whole virus lysates of HIV-1 or of HIV-1 and HIV-2, and of two tests based on agglutination of sensitized particles. A total of 734 West African sera were screened. The best results were obtained with tests using recombinant proteins. HIV tests using synthetic peptides able to distinguish between HIV-1 and HIV-2 antibodies showed comparable specificity, but some false negative results represent a major handicap of these peptide EIAs in screening for HIV antibodies.

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Authors and Affiliations

  1. Département de Virologie, Centre Hospitalier Universitaire Dupuytren, 87042, Limoges Cedex, France

    G. Léonard, M. Verdier & F. Denis

  2. Institut Pasteur, Abidjan, Ivory Coast

    A. Sangaré

  3. Organisme de Recherche Scientifique et Technique d'Outre Mer, Montpellier, France

    J. L. Rey

Authors
  1. G. Léonard
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  2. M. Verdier
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  3. A. Sangaré
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  4. J. L. Rey
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  5. F. Denis
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Léonard, G., Verdier, M., Sangaré, A. et al. Specificity of new peptide immunoassays versus other immunoassays and agglutination tests for detection of HIV antibody in African Sera. Eur. J. Clin. Microbiol. Infect. Dis. 10, 636–640 (1991). https://doi.org/10.1007/BF01975815

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