Abstract
Since little information has been reported about the specificity of antibodies to human immunodeficiency virus type 1 (HIV-1) found in the cerebrospinal fluid (CSF), 21 CSF and serum specimens were examined from 19 patients with clinical AIDS, AIDS-related complex, or asymptomatic HIV-1 infection. The predominant specificity of antibodies using Western blot analysis in both serum (100 %) and CSF (100 %) was directed towardenv gene products. The next most common antibody specificities were to thepol gene products (serum 95 %; CSF 62 %). Less commonly found was antibody to thegag-encoded proteins (serum 71 %; CSF 38 %). The level of antibody to HIV-1 in CSF could not be predicted from the level found in serum. Also, the spectrum of antibodies seen did not correlate with disease stage or with the quantity of antibody present. The serum/CSF pairs were also examined for the presence of HIV-1 antigen by commercial enzyme immunoassay. HIV-1 antigen was present in eight of 19 (43 %) of the serum samples and five of 20 (25 %) of the CSF samples tested.
Similar content being viewed by others
References
Klatzmann, D., Barre-Sinoussi, F., Nugeyre, C., Dauguet, C., Vilmer, E., Griscelli, C., Brun-Vezinet, F., Rouzioux, C., Gluckman, J. C., Chermann, J.-C., Montagnier, L. Selective tropism of lymphadenopathy associated virus for helper-inducer T lymphocytes. Science 1984, 225: 59–63.
Gartner, S., Markovits, P., Markovits, D. M., Kaplan, M. H., Gallo, R. C., Popovic, M. The role of mononuclear phagocytes in HTLV-III/LAV infection. Science 1986, 233: 215–219.
Wiley, C. A., Schrier, R. D., Nelson, J. A., Lampert, P. W., Oldstone, M. B. A. Cellular localization of human immunodeficiency virus infection within the brains of acquired immune deficiency syndrome patients. Proceedings of the National Academy of Science of the USA 1986, 83: 7089–7093.
McArthur, J. C., Cohen, B. A., Farzedegan, H., Cornblath, D. R., Selnes, O. A., Ostrow, D., Johnson, R. T., Phair, J., Polk, B. F. Cerebrospinal fluid abnormalities in homosexual men with and without neuropsychiatric findings. Annals of Neurology 1988, 23, Supplement: 34–37.
Hollander, H., Levy, J. A. Neurologic abnormalities and recovery of human immunodeficiency virus from cerebrospinal fluid. Annals of Internal Medicine 1987, 106: 692–695.
Albert, J., Gaines, H., Sonnerborg, A., Nystrom, G., Pehrson, P. O., Chiodi, F., von Sydow, M., Moberg, L., Lidman, K., Christensson, B., Asjol, B., Fenyo, E. A. Isolation of human immunodeficiency virus from plasma during primary HIV infection. Journal of Medical Virology 1987, 23: 67–73.
McAllister, R. H., Harrison, M. J. G. HIV and the nervous system. British Journal of Hospital Medicine 1988, 39: 21–26.
Price, R. W., Brew, B., Sidtis, J., Rosenblum, M., Schech, A. C., Cleary, P. The brain in AIDS: central nervous system HIV-1 infection and AIDS dementia complex. Science 1988, 239: 586–592.
Levy, R. M., Bredesen, D. E. Central nervous system dysfunction in acquired immunodeficiency syndrome. Journal of the Acquired Immunodeficiency Syndrome 1988, 1: 41–64.
Ho, D. D., Rota, T. R., Schooley, R. T., Kaplan, J. C., Allan, D., Groopman, J. E., Resnick, L., Felsenstein, D., Andrews, C. A., Hirsch, M. S. Isolation of HTLV-III from cerebrospinal fluid and neural tissues of patients with neurologic syndromes related to the acquired immunodeficiency syndrome. New England Journal of Medicine 1985, 313: 1493–1497.
Shaw, G. M., Harper, M. E., Hahn, B. H., Epstein, L. G., Gajdusek, D. C., Price, R. W., Navia, B. A., Petito, C. K., O'Hara, C. J., Groopman, J. E., Cho, E.-S., Oleske, J. M., Wong-Staal, F., Gallo, R. C. HTLV-III infection in brains of children and adults with AIDS encephalopathy. Science 1985, 227: 177–181.
Goudsmit, J., Lange, J. M. A., Krone, W. J. A., Teunissen, M. B. M., Epstein, L. G., Danner, S. A., van den Berg, H., Breederveld, C., Smit, L., Bakker, M., de Wolf, F., Coutinho, R. A., van der Noordaa, J. Pathogenesis of HIV and its implications for serodiagnosis and monitoring antiviral therapy. Journal of Virological Methods 1987, 17: 19–34.
Resnick, L., Berger, J. R., Shapshak, P., Tourtellotte, W. W. Early penetration of the blood-brain barrier by HIV. Neurology 1988, 38: 9–14.
Resnick, L., diMarzo-Veronese, F., Schupbach, J., Tourtellotte, W. W., Ho, D. D., Muller, F., Shapshak, P., Vogt, M., Groopman, J. E., Markham, P. D., Gallo, R. C. Intra-blood-brain barrier synthesis of HTLV-III-specific IgG in patients with neurologic symptoms associated with AIDS or AIDS-related complex. New England Journal of Medicine 1985, 313: 1498–1504.
Goudsmit, J., Wolters, E. C., Bakker, M., Smit, L., van der Noordaa, J., Hische, E. A. H., Tutuarima, J. A., van der Helm, H. J. Intrathecal synthesis of antibodies to HTLV-III in patients without AIDS or AIDS-related complex. British Journal of Medicine 1986, 292: 1231–1234.
Goudsmit, J., Paul, D. A., Lange, J. M. A., Speelman, H., van der Noordaa, J., van der Helm, H. J., de Wolf, F., Epstein, L. G., Krone, W. J. A., Wolters, E. C., Oleske, J. M., Coutinho, R. A. Expression of human immunodeficiency virus antigen in serum and cerebrospinal fluid during acute and chronic infection. Lancet 1986, ii: 177–180.
Ackerman, R., Nekic, M., Jurgens, R. Locally synthesized antibodies in cerebrospinal fluid of patients with AIDS. Journal of Neurology 1986, 233: 140–141.
Biniek, R., Bartholome, M., Schulz, M., Lehmann, H. J., Gesemann, H., Scheiermann, N., Brockmeyer, N. H., Derks, M., Balzer, K. Intrathecal production of HIV antibodies in suspected AIDS encephalopathy. Journal of Neurology 1988, 235: 131–135.
Gallo, P., De Rossi, A., Amadori, A., Tavolato, B., Chieco-Bianchi, L. Central nervous system involvement in HIV infection. AIDS Research and Human Retroviruses 1988, 4: 211–221.
Centers for Disease Control Revision of the case definition of acquired immunodeficiency syndrome for national reporting — United States. Morbidity and Mortality Weekly Report 1985, 34: 373–375.
Centers for Disease Control Human immunodeficiency virus (HIV) infection codes official authorized addendum ICM-9-CM. Morbidity and Mortality Weekly Report 1987, 36, Supplement: 1–20.
Consortium for Retrovirus Serology Standardization Serological diagnosis of human immunodeficiency virus infection by Western blot testing. Journal of the American Medical Association 1988, 260: 674–679.
Centers for Disease Control Interpretation and use of the Western blot assay for serodiagnosis of human immunodeficiency virus type 1 infections. Morbidity and Mortality Weekly Report 1989, 38, Supplement 7: 1–7.
Beckett, A., Summergrad, P., Manschreck, T., Vitagliano, H., Henderson, M., Buttolph, M. L., Jenike, M. Symptomatic HIV infection of the CNS in a patient without clinical evidence of immune deficiency. American Journal of Psychiatry 1987, 144: 1342–1344.
Mergener, K., Enzensberger, W., Rubsamen-Waigmann, H., von Briesen, H., Doerr, H. W. Immunoglobulin class- and subclass-specific HIV antibody detection in serum and CSF specimens by ELISA and Western blot. Infection 1987, 15: 317–322.
Johnson, J. E., Zolla-Pazner, S. Detection of HTLV-III antibody in cerebrospinal fluid from patients with AIDS and pre-AIDS with the use of a commercial test system. American Journal of Clinical Pathology 1987, 88: 351–353.
von Briesen, H., Becker, W. B., Henco, K., Helm, E. B., Gelderblom, H. R., Brede, H. D., Rubsamen-Waigmann, H. Multiple simultaneous variants in a patient demonstrated by molecular cloning. Journal of Medical Virology 1987, 23: 51–66.
Homsy, J., Thomson-Honnebier, G. A., Cheng-Mayer, C., Levy, J. A. Detection of human immunodeficiency virus (HIV) in serum and body fluids by sequential competition ELISA. Journal of Virological Methods 1988, 19: 43–56.
Tsiquave, K. N., Youle, M., Chanas, A. C. Restriction of sensitivity of HIV-1 antigen ELISA by serum anticore antibodies. AIDS 1988, 2: 41–45.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Willoughby, P.B., Midgett, J.S. & Folds, J.D. Antibody specificity for human immunodeficiency virus type 1 in serum and cerebrospinal fluid from patients with AIDS and AIDS-related complex. Eur. J. Clin. Microbiol. Infect. Dis. 8, 1031–1037 (1989). https://doi.org/10.1007/BF01975164
Issue Date:
DOI: https://doi.org/10.1007/BF01975164