Abstract
A new isolation procedure of rat C-reactive protein (CRP) by affinity chromatography on aminocoupled phosphorylcolamine-Sepharose is described. CRP serum level of rats injured by turpentine injection or two different arthritis models was determined to test the potency of this protein as an marker of inflammation. The influence of repeated adminstration of carbon tetrachloride as a hepatotoxic agent was investigated in the same way.
It was shown that CRP determination is a useful method to observe the course of experimental inflammations and other tissue injuries as well.
Similar content being viewed by others
References
M. B. Pepys, A. C. Dash, T. C. Fletcher, N. Richardson, E. A. Mumm and A. Feinstein,Analogues in other mammals and fish of human plasma proteins, C-reactive protein, and amyloid P-component. Nature273, 168–170 (1978).
F. C. de Beer, M. L. Baltz, E. A. Mumm, A. Feinstein, J. Taylor, C. Bruton, J. R. Clamp and M. B. Pepys,Isolation and characterization of C-reactive protein and serum amyloid P-component in the rat. Immunology45, 55–70 (1982).
M. Pontet, M. D. Asnieres, D. Gache, J. Escaig and R. Engler,A new pentraxine (serum amyloid-P-component) in the rat: Evidence for two quaternary structures and effect of ligands on self-association. Biochim. Biophys. Acta.671, 202 (1981).
C. B. Laurell,Electroimmunoassay. Scand. J. Clin. Lab. Invest.29, 39 (1972).
R. Hirschelmann, R. Schade and H. Bekemeier,The influence of inhibitors of RNA and protein synthesis on dexamethasone action in carrageenin paw edema and adjuvant arthritis of rats. Agents and Actions, Suppl.10, 233–240 (1982).
R. Hirschelmann, R. Schade and H. Bekemeier,6-sulphanilamidoindazole arthritis of rats: relation between acute-phase proteins, degree of arthritis and treatment with Soybeantrypsin inhibitor. Agents and Actions10, 431–434 (1980).
M. B. Pepys, M. Baltz, K. Gomer, A. J. S. Davies and M. Doenhoff,Serum amyloid P-component is an acute-phase reactant in the mouse. Nature278, 259–261 (1979).
J. E. Coe,Homologs of CRP: A diverse family of proteins with similar structure. In:Contemporary topics in molecular immunology, vol. 9 (Eds. F. P. Inman and T. J. Kindt) pp. 211–238, Plenum Publishing Corporation 1983.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bürger, W., Schade, R. & Hirschelmann, R. The rat C-reactive protein — isolation and response to experimental inflammation and tissue damage. Agents and Actions 21, 93–97 (1987). https://doi.org/10.1007/BF01974928
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01974928