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Evaluation of a commercial DNA probe assay for the identification ofMycobacterium kansasii

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Abstract

A commercially available DNA probe (the Accu-ProbeMycobacterium kansasii culture identification test, Gen-Probe, USA) for the identification ofMycobacterium kansasii was tested on a panel of 143 fully characterized mycobacterial strains. The isolates included 70Mycobacterium kansasii and 73 mycobacteria other thankansasii. The specificity was 100 % while the sensitivity was 72.8 %. This sensitivity is unusually low in comparison with that of commercial DNA probes for other mycobacteria and confirms a previous study that found genetic heterogeneity within the speciesMycobacterium kansasii. Strains that do not hybridize with the AccuProbe are particularly prevalent in Italy and perhaps elsewhere in Europe.

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Authors and Affiliations

  1. Microbiological and Virological Laboratory, Careggi Hospital, viale Pieraccini 24, 50139, Florence, Italy

    E. Tortoli & M. T. Simonetti

  2. Institute for Chest Disease, TB Laboratory, Milan, Italy

    C. Lacchini & V. Penati

  3. Department of Clinical Microbiology, Umberto I-Torrette Hospital, Ancona, Italy

    C. Piersimoni & V. Morbiducci

Authors
  1. E. Tortoli
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  2. M. T. Simonetti
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  3. C. Lacchini
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  4. V. Penati
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  5. C. Piersimoni
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  6. V. Morbiducci
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Tortoli, E., Simonetti, M.T., Lacchini, C. et al. Evaluation of a commercial DNA probe assay for the identification ofMycobacterium kansasii . Eur. J. Clin. Microbiol. Infect. Dis. 13, 264–267 (1994). https://doi.org/10.1007/BF01974549

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  • DOI: https://doi.org/10.1007/BF01974549

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