Abstract
The ionophore X537A induced swelling of isolated rat mast cells parallel to histamine release. Both actions were depressed by extracellular calcium and BSA, temperatures below 37°C, NEM, PMSF, and TTX, and were enhanced by high potassium and pretreatment of the cells with ATP. DSCG, theophylline, and DFP enhanced the histamine release noted after 10 min of incubation without influencing the swelling action of X537A. The swelling action could not be separated from histamine release and it is suggested that it right be inherent in the mechanism of secretion induced by X537A. The present results further distinguish histamine release induced by the two ionophores X537A and A23187.
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P. Anderson, S.A. Slorach andB. Uvnäs,Sequential Exocytosis of Storage Granules during Antigen-Induced Histamine Release from Sensitized Rat Mast Cells in vitro. An Electron Microscopic Study, Acta physiol. scand.88, 359–372 (1973).
G.D. Bloom andÖ. Hägermark,A Study on Morphological Changes and Histamine Release Induced by Compound 48/80 in Rat Peritoneal Mast Cells, Exp. Cell Res.40, 637–654 (1965).
G.D. Bloom, B. Diamant, Ö. Hägermark andM. Ritzén,The Effects of Adenosine-5′-triphosphate (ATP) on Structure and Amine Content of Rat Peritoneal Mast Cells, Exp. Cell Res.62, 61–75 (1970).
D.E. Cochrane andW.W. Douglas,Calcium-Induced Extrusion of Secretory Granules (Exocytosis) in Mast Cells Exposed to 48/80 or the Ionophores A-23187 and X537A, Proc. Nat. Acad. Sci. USA71, 408–412 (1974).
G. Cornelius, W. Gärtner andD.H. Haynes,Cation Complexion by Valinomycin- and Nigericin-Type Ionophores Registered by the Fluorescence Signal of T1 +, Biochemistry13, 3052–3057 (1974).
R. Dahlquist, B. Diamant andK. Elwin,Interaction of Adenosine-5′-triphosphate (ATP) with Histamine Release Induced by Compound 48/80, Acta physiol. scand.87, 145–157 (1973).
R. Dahlquist, B. Diamant andP.G. Krüger,Increased Permeability of Rat Mast Cell Membrane to Sodium and Potassium caused by Extracellular ATP and its Relationship to Histamine Release, Int. Archs Allergy appl. Immun.46, 655–675 (1974).
B. Diamant andP.G. Krüger,Histamine Release from Isolated Rat Peritoneal Mast Cells Induced by Adenosine-5′-triphosphate, Acta physiol. scand.71, 291–302 (1967).
B. Diamant andS.A. Patkar,Stimulation and Inhibition of Histamine Release from Isolated Rat Mast Cells. Dual Effects of the Ionophore A23187, Int. Archs Allergy appl. Immun.49, 183–207 (1975).
B. Diamant, W. Kazimierczak andS.A. Patkar,Mechanism of Histamine Release Induced by the Ionophore X537A from Isolated Rat Mast Cells. III. Actions of X537A on Isolated Histamine-Retaining Granules, on a Heparin-Protamine Complex Saturated with Histamine and on Transport of Histamine into an Organic Phase, Int. Archs Allergy appl. Immun.56, 179–185 (1978).
J.C. Foreman, J.L. Mongar andB.D. Gomperts,Calcium Ionophores and Movement of Calcium Ions Following the Physiological Stimulus to a Secretory Process, Nature245, 249–251 (1973).
N. Grosman andB. Diamant,Effect of Adenosine-5′-triphosphate (ATP) on Rat Mast Cells: Influence on Anaphylactic and Compound 48/80-Induced Histamine Release, Agents and Actions5, 108–114 (1975).
N. Grosman andB. Diamant,Studies on Histamine-Retaining Granules Obtained from Isolated Rat Mast Cells, Agents and Actions6, 394–401 (1976).
M. Kagayama andW.W. Douglas,Electron Microscope Evidence of Calcium-Induced Exocytosis in Mast Cells Treated with 48/80 or the Ionophores A-23187 and X-537A, J. Cell Biol.62, 519–525 (1974).
W. Kazimierczak, S.A. Patkar andB. Diamant,The Mechanism of Histamine Release Induced by the Ionophore X537A from Isolated Rat Mast Cells. I. Significance of Monovalent Cations, Calcium, Metabolic Energy and Temperature, Acta physiol. scand.102, 265–273 (1978).
P.G. Krüger andG.D. Bloom,Granule Alteration and Vacuole Formation, Two Primary Structural Features of the Histamine Release Process in Mast Cells, Experientia,29, 329–330 (1973).
D. Lagunoff,Membrane Fusion during Mast Cell Secretion, J. Cell Biol.57, 252–259 (1973).
D.C. Morrison, J.F. Roser, G.C. Cochrane andP.M. Henson,Two Distinct Mechanisms for the Initiation of Mast Cell Degranulation, Int. Archs Allergy appl. Immun.49, 172–178 (1975).
D.C. Morrison, J.F. Roser, P.M. Henson andC.G. Cochrane,Isolation and Characterization of a Non-Cytotoxic Mast Cell Activator from Cobra Venom, Inflammation,1, 103–115 (1975).
D.C. Morrison, P.M. Henson, J.F. Roser andC.G. Cochrane, in:Leukocyte Membrane Determinants Regulating Immune Reactivity (Academic Press, New York, 1976), p. 551.
S.A. Patkar andB. Diamant The Action of Various Histamine Releasing Agents on the Volume Distribution of Rat Mast Cells Registered by the Coulter Channelyzer, Agents and Actions5, 465 (1975).
B.C. Pressman,Properties of Ionophores with Broad Range Cation Selectivity, Fed. Proc.32, 1698–1703 (1973).
P. Röhlich,Membrane-Associated Actin Filaments in the Cortical Cytoplasm of the Rat Mast Cell, Exp. Cell Res.93, 293–298 (1975).
P. Röhlich, P. Anderson andB. Uvnäs,Electron Microscope Observations on Compound 48/80-Induced Degranulation in Rat Mast Cells. Evidence for Sequential Exocytosis of Storage Granules, J. Cell Biol.51, 465–483 (1971).
B. Uvnäs, C.-H. Åborg andA. Bergendorff,Storage of Histamine in Mast Cells. Evidence for an Ionic Binding of Histamine to Protein Carboxyls in the Granule Heparin-Protein Complex Acta physiol. scand.78, Suppl. 336 (1970).
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Diamant, B., Kazimierczak, W. & Patkar, S.A. The mechanism of histamine release induced by the ionophore X537A from isolated rat mast cells. II. The relationship between increase of cell volume and histamine release. Agents and Actions 8, 315–323 (1978). https://doi.org/10.1007/BF01968610
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DOI: https://doi.org/10.1007/BF01968610