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Effect of time elapsed since previous voiding on the detection ofChlamydia trachomatis antigens in urine

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Abstract

To determine if the time elapsed since previous voiding affects the sensitivity of an enzyme immunoassay (EIA) to detectChlamydia trachomatis in urine, 882 women and 428 men were tested for chlamydial infection in urethral specimens by isolation in cell culture (women and men) and EIA with blocking confirmation (women only). Of the 36 women (4.1 %) and 38 men (8.9 %) who were positive forChlamydia trachomatis in the urethra, 55.5 % (20/36) and 81.6 % (31/38) respectively were positive in the first void urine (FVU) sediment by confirmed EIA. In women the sensitivity of the EIA performed on FVU was 67.8 % (19/28) if the urine had been in the bladder <3 hours and decreased to 12.5 % (1/8) if longer times had elapsed (odds ratio 13.7; 95 % confidence interval 1.4 to 700.0) with no obvious confounding. In men a weaker association was present (odds ratio 2.7; 95 % confidence interval 0.4 to 22.3). These findings should enable investigators to optimize the analysis of urine to diagnose chlamydial infections.

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Authors and Affiliations

  1. Hamilton-Wentworth Department of Public Health Services, McMaster University, Hamilton, Ontario, Canada

    J. Sellors, L. Pickard & S. Walter

  2. Department of Clinical Epidemiology and Biostatistics, McMaster University, Hamilton, Ontario, Canada

    J. Sellors, L. Pickard & S. Walter

  3. Department of Pediatrics, McMaster University, Hamilton, Ontario, Canada

    M. Chernesky

  4. Department of Pathology, McMaster University, Hamilton, Ontario, Canada

    M. Chernesky & J. Mahony

  5. Gamma-North Peel Laboratory, North York, Ontario, Canada

    J. Krepel

  6. Regional Virology and Chlamydiology Laboratory, St. Joseph's Hospital, 50 Charlton Avenue East, L8N, 4A6, Hamilton, Ontario, Canada

    J. Sellors, M. Chernesky, D. Jang & J. Mahony

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  1. J. Sellors
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  2. M. Chernesky
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  3. L. Pickard
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  4. D. Jang
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  5. S. Walter
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  6. J. Krepel
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  7. J. Mahony
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Sellors, J., Chernesky, M., Pickard, L. et al. Effect of time elapsed since previous voiding on the detection ofChlamydia trachomatis antigens in urine. Eur. J. Clin. Microbiol. Infect. Dis. 12, 285–289 (1993). https://doi.org/10.1007/BF01967260

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  • DOI: https://doi.org/10.1007/BF01967260

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