Summary
Treatment of turkey liver fructose-1,6-bisphosphatase with penicillin G progressively inactivated the enzyme and desensitized the enzyme toward high substrate inhibition. The treatment also led to reduced sensitivity to AMP inhibition and the loss of cooperative interaction among AMP-binding sites. These altered properties were not reversed by dialysis, but were prevented when treatment with penicillin G was performed in the presence of substrate.
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This work was in part supported by grant RR-8006 from the General Research Branch, Division of Research Resources, NIH (USA) and in part by Faculty Research Grant from Southern School of Pharmacy, Mercer University, Atlanta (Georgia, USA).
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Han, P.F., Pollock, S.H., Matthews, H.W. et al. Functional consequences of treating turkey liver fructose-1,6-bisphosphatase with penicillin G. Experientia 35, 586–587 (1979). https://doi.org/10.1007/BF01960333
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DOI: https://doi.org/10.1007/BF01960333