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Pre-and post-natal ontogeny of neutral endopeptidase 24-11 (‘enkephalinase’) studied by in vitro autoradiography in the rat

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Abstract

Neutral endopeptidase (NEP, enkephalinase, CALLA) which is present in various neural and non-neural tissues, is able to cleave a variety of regulatory peptides. The distribution of NEP has been studied during rat pre-and post-natal development by autoradiography after in vitro binding of the tritiated inhibitor [3H]HACBO-Gly to whole-body and organ sections. In the central nervous system (CNS), where the presence of NEP has been related to the termination of the action of enkephalins, the external layer of the olfactory bulbs is the only structure prominently labeled before birth. Other CNS structures rich in NEP in the adult, such as the nigrostriatal tract, are progressively labeled after birth. Outside the CNS, the progressive appearance of NEP in the kidney, the lungs and the salivary glands suggests its concomitant involvement in adult physiological functions, including fluid balance control, possibly by cleaving the atrial natriuretic peptide (ANP) and other peptides. On the other hand, transient or enhanced expression of NEP is observed during the development of several organs such as the sensory organs, the heart and the major blood vessels, the intestine, the bones and the genital tubercle. In addition to the still incompletely known physiological functions of the enzyme, the developmental pattern of its expression in several tissues strongly suggests a modulatory role for NEP in the ontogeny of a large number of organs.

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Literatur

  1. Roques, B. P., Beaumont, A., Dauge, V., and Fournié-Zaluski, M. C., in: Handbook of Experimental Pharmacology. Eds G. V. R. Born, P. Cuatrecasas, H. Herken and A. Schwartz. Springer Verlag, Heidelberg (1991) in press.

    Google Scholar 

  2. Turner, A. J., in: Neuropeptides and Their Peptidases, pp. 183–198. Ed. A. J. Turner. Ellis Horwood series in Biomedicine, VCH Publishers 1987.

  3. Stephenson, S. L., and Kenny, A. J., Biochem. J.243 (1987) 183.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Sakurada, C., Yokosawa, H., and Ishii, S. I., Peptides11 (1990) 287.

    Article  CAS  PubMed  Google Scholar 

  5. Sokolovsky, M., Galron, R., Kloog, Y., Bdolah, A., Indig, F. E., Blumberg, S., and Fleminger, G., Proc. natl Acad. Sci. USA87 (1990) 4702.

    Article  CAS  Google Scholar 

  6. Pierart, M. E., Najidovski, T., Appelboom, T. E., and Deschodt-Lanckman, M. M., J. Immun.140 (1988) 3808.

    Article  CAS  PubMed  Google Scholar 

  7. Waksman, G., Hamel, E., Fournié-Zaluski, M. C., and Roques, B. P., Proc. natl Acad. Sci. USA83 (1986) 1523.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Salès, N., Charnay, Y., Zajac, J. M., Dubois, P. M., and Roques, B. P., J. chem. Neuroanat.2 (1989) 179.

    PubMed  Google Scholar 

  9. Salès, N., Dutriez, I., Mazière, B., Ottaviani, M., and Roques, B. P., Reg. Pept.33 (1991) 209.

    Article  Google Scholar 

  10. Thorsett, E. D., and Wyvratt, M. T. in: Neuropeptides and Their Peptidases, p. 229. Ed. A. J. Turner. Ellis Horwood series in Biomedicine, VCH Publishers 1987.

  11. Roques, B. P., Fournié-Zaluski, M. C., Soroca, E., Lecomte, J. M., Malfroy, B., Llorens, C., and Schwartz, J. C., Nature (London)288 (1980) 286.

    Article  CAS  PubMed  Google Scholar 

  12. Roques, B. P., and Beaumont, A., Trends pharmac. Sci.11 (1990) 245.

    Article  CAS  Google Scholar 

  13. Koehn, J. A., Norman, J. A., Jones, B. N., Le Sueur, L., Sakane, Y., and Ghai, R. D., J. biol. Chem.262 (1987) 11623.

    Article  CAS  PubMed  Google Scholar 

  14. Kenny, A. J., Stephenson, S. L., and Turner, A. J., in: Mammalian Ectoenzymes, p. 169. Eds A. J. Kenny and A. J. Turner. Elsevier, London 1987.

    Google Scholar 

  15. Waksman, G., Hamel, E., Delay-Goyet, P., and Roques, B. P., Brain Res.436 (1987) 205.

    Article  CAS  PubMed  Google Scholar 

  16. Zajac, J. M., Lombard, M. C., Pechanski, M., Besson, J. M., and Roques, B. P., Brain Res.477 (1989) 400.

    Article  CAS  PubMed  Google Scholar 

  17. Zajac, J. M., Charnay, Y., Soleilhac, J. M., Salès, N., and Roques, B. P., FEBS Lett.216 (1987) 118.

    Article  CAS  PubMed  Google Scholar 

  18. Zajac, J. M., and Roques, B. P., in: brain Imaging. Techniques and Applications, p. 18. Eds N. A. Sharif and M. E. Lewis. Ellis Horwood Limited, John Wiley & Sons, New York 1989.

    Google Scholar 

  19. Davila-Garcia, M. I., and Azmitia, E. C., Dev. Brain Res.49 (1989) 97.

    Article  CAS  Google Scholar 

  20. Waksman, G., Bouboutou, R., Devin, G., Besselievre, R., Fournié-Zaluski, M. C., and Roques, B. P., Biochem. biophys. Res. Commun.131 (1985) 262.

    Article  CAS  PubMed  Google Scholar 

  21. Ronco, P., Pollard, H., Galceran, M., Delauche, M., and Schwartz, J. C., Lab. Invest.58 (1988) 210.

    CAS  PubMed  Google Scholar 

  22. Matsas, R., Kenny, A. J., and Turner, A. J., Neuroscience18 (1986) 991.

    Article  CAS  PubMed  Google Scholar 

  23. Gee, N. S., Bowes, M. A., Buck, P., and Kenny, A. J., Biochem. J.228 (1985) 119.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Johnson, A. R., Gray, L. D., Youngblood, E., and Sullivan, J., J. cell. Biochem.43 (1990) 243.

    Article  CAS  PubMed  Google Scholar 

  25. Lecavalier, H., Crine, P., and Malo, C., Biol. Neonate56 (1989) 342.

    Article  CAS  PubMed  Google Scholar 

  26. Marcais-Collado, H., Uchida, G., Costentin, J., Schwartz, J. C., and Lecomte, J. M., Eur. J. Pharmac.144 (1987) 125.

    Article  CAS  Google Scholar 

  27. Kent, J. L., Pert, C. B., and Herkhenham, M., Devl. Brain Res2 (1982) 487.

    Article  Google Scholar 

  28. Spain, J. W., Roth, B. L., and Coscia, C. J., J. Neurosci.5 (1985) 584.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Tong, Y., and Pelletier, G., Neuropeptides16 (1990) 63.

    Article  CAS  PubMed  Google Scholar 

  30. Tervo, K., Tervo, T., Eranko, L., and Cuello, A. C., Histochem. J.13 (1981) 435.

    Article  CAS  PubMed  Google Scholar 

  31. Stone, R. A., and Kuwayama, Y., Archs Ophtal.103 (1985) 1207.

    Article  CAS  Google Scholar 

  32. Stone, R. A., Kuwayama, Y., and Laties, A. M., Experientia43 (1987) 790.

    Article  Google Scholar 

  33. Palm, D. E., Keil, L. C., Sassani, J. W., and Severs, W. B., Brain Res.504 (1989) 142.

    Article  CAS  PubMed  Google Scholar 

  34. Lange, W., Lang, R., Basting, C., and Unger, J. W., Exp. Eye Res.50 (1990) 313.

    Article  CAS  PubMed  Google Scholar 

  35. Anderson, J. A., Malfroy, B., Richard, N. R., Kullerstrand, R., Lucas, C., and Binder, P. S., Regulatory Peptides29 (1990) 49.

    Article  CAS  PubMed  Google Scholar 

  36. Barnes, P. L., Experientia43 (1987) 832.

    Article  CAS  PubMed  Google Scholar 

  37. Goodlats, R. A., and Wright, N. A., Experientia43 (1987) 780.

    Article  Google Scholar 

  38. Lehy, J., Puccio, F., Chariot, J., and Labeille, D., Gastroenterology90 (1986) 1942.

    Article  CAS  PubMed  Google Scholar 

  39. LeBien, T. W., and McCormack, R. T., Blood73 (1989) 625.

    Article  CAS  PubMed  Google Scholar 

  40. Vollmar, A. M., Klin. Wochenschr.68 (1990) 699.

    Article  CAS  PubMed  Google Scholar 

  41. Marala, R. B., and Sharma, R. K., Biochem. J.251 (1988) 301.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  42. Göke, R., Göke, P., Noll, B., Richter, G., Fehmann, H. C., and Arnold, R., Biomedical Res.10 (1989) 463.

    Article  Google Scholar 

  43. Okamura, H., Kelly, P. A., Chabot, J. G., Morel, G., Belles-Isles, M., and Heisler, S., biochem. biophys. Res. Commun.156 (1988) 1000.

    Article  CAS  PubMed  Google Scholar 

  44. Fletcher, A. E., Allan, E. H., Casley, D. J., and Martin, T. J., FEBS Lett.208 (1986) 263.

    Article  CAS  PubMed  Google Scholar 

  45. Bruns, C., Dietl, M. M., Palacios, J. M., and Pless, J., Biochem. J.265 (1990) 39.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  46. Valet, M., Berlan, M., Beauville, M., Crampes, F., Montastruc, J. L., and Lafontan, M., J. clin. Invest.85 (1990) 291.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  47. Smith, F. G., Sato, T., Varille, V. A., and Robillard, J. E., J. devl Biol.12 (1989) 55.

    CAS  Google Scholar 

  48. Tamburini, P. P., Kochn, J. A., Gilligan, J. P., Charles, D., Palmesino, R. A., Sharif, R., McMartin, C., Erion, M. D., and Miller, M. J. S., Pharm. exp. Ther.251 (1989) 956.

    CAS  Google Scholar 

  49. Soleilhac, J. M., Lucas, E., Beaumont, A., Turcaud, S., Michel, J. B., Crine, P., Fournié-Zaluski, M. C., and Roques B. P., Molec. Pharmac. (1991), submitted.

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Authors and Affiliations

  1. DRIPP Service hospitalier Frédéric Joliot, place du Général Leclerc, INSERM U 334, F-91406, Orsay, France

    I. Dutriez, N. Salès, M. -C. Fournié-Zaluski & B. P. Roques

  2. Département de Chimie Organique, 4 avenue de l'Observatoire, INSERM U 266 CNRS UA 498, F-75270, Paris Cedex 06, France

    I. Dutriez, N. Salès, M. -C. Fournié-Zaluski & B. P. Roques

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  1. I. Dutriez
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  2. N. Salès
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  3. M. -C. Fournié-Zaluski
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  4. B. P. Roques
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Dutriez, I., Salès, N., Fournié-Zaluski, M.C. et al. Pre-and post-natal ontogeny of neutral endopeptidase 24-11 (‘enkephalinase’) studied by in vitro autoradiography in the rat. Experientia 48, 290–300 (1992). https://doi.org/10.1007/BF01930479

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