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Carbodiimide fixation for electron microscopy and immunoelectron cytochemistry

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Zusammenfassung

In mit wasserlöslichen Carbodiimiden unter bestimmten Bedingungen fixiertem Gewebe können Polypeptidhormone immunfluoreszenzoptischnachgewiesen werden. Unter optimalen Bedingungen fixiertes Material ist auch zur Herstellung elektronenmikroskopischer Präparate vorzüglich geeignet. Carbodiimide werden deshalb als Fixierungsmittel in elektronenoptischen, immunzytochemischen Studien vorgeschlagen.

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References

  1. P. A. Kendall, Julia M. Polak andA. G. E. Pearse, Experientia,27, 1104 (1971).

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Authors and Affiliations

  1. Department of Histochenmistry, Royal Postgraduate Medical School, W12 OHS, London, England

    Julia M. Polak, P. A. Kendall, C. M. Heath & A. G. E. Pearse

Authors
  1. Julia M. Polak
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  2. P. A. Kendall
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  3. C. M. Heath
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  4. A. G. E. Pearse
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Additional information

We wish to thank Dr.S. Bloom for preparing the anti-percine secretin used in this study. This work was supported by the Wellcome Trust and the Cancer Research Campain.

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Polak, J.M., Kendall, P.A., Heath, C.M. et al. Carbodiimide fixation for electron microscopy and immunoelectron cytochemistry. Experientia 28, 368–370 (1972). https://doi.org/10.1007/BF01928746

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