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Inhibition of tumor cell growth by a human B-cell line

  • Published:
Biotherapy

Abstract

An Adenocarcinoma cell line (Breast-M) and an Epstein-Barr virus (EBV)-infected B-cell line (Hairy-BM) were established from breast tumor tissue. The Hairy-BM was CD20+, CD25 (Tac)+ and surface immunoglobulin (sIg)+. Hairy-BM suppressed the in vitro proliferation of Breast-M in a time and a dose-dependent manner. The suppression was also found in 5 different human tumor targets showing tumor-Hairy-BM binding, but not; in 2 murine tumor targets showing no significant tumor-Hairy-BM binding. Lytic activity of Hairy-BM was found only against Breast-M.

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Abbreviations

sIg :

Surface immunoglobulin

CTL :

Cytotoxic T-cells

NK :

Natural killer

IL2 :

Interleukin 2

LAK :

Lymphokine activated killer

CSN :

Culture supernatant

MTT :

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoolium bromide

PCR :

Polymerase chain reaction

TIL :

Tumor-infiltrating lymphocytes

HCL :

Hairy cell leukemia

TNF :

Tumor necrosis factor

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Authors and Affiliations

  1. Department of Surgery, Saga Medical School, 5-1-1 Nabeshima, 849, Saga, Japan

    Mitsuo Katano, Tatsuya Matsuo & Takeharu Hisatsugu

  2. Department of Oral and Maxillofacial Surgery, Saga Medical School, 5-1-1 Nabeshima, 849, Saga, Japan

    Eiro Kubota

  3. Hospital Laboratory, Saga Medical School, 5-1-1 Nabeshima, 849, Saga, Japan

    Fumio Nagumo & Jutaro Tadano

Authors
  1. Mitsuo Katano
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  2. Eiro Kubota
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  3. Fumio Nagumo
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  4. Tatsuya Matsuo
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  5. Takeharu Hisatsugu
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  6. Jutaro Tadano
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Katano, M., Kubota, E., Nagumo, F. et al. Inhibition of tumor cell growth by a human B-cell line. Biotherapy 8, 1–6 (1994). https://doi.org/10.1007/BF01878115

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  • DOI: https://doi.org/10.1007/BF01878115

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