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X-Ray small-angle scattering from concentrated solutions of normal and sickle cell deoxyhemoglobin

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Abstract

X-ray small-angle scattering of human normal and sickle cell deoxyhemoglobin in aqueous solution was measured at various concentrations, including those approximating the intracellular. The calculated radius of gyration, determination of the overall shape, and Soulé-Porod plots all indicate a concentration-dependent difference in strength of interparticle forces between normal and sickle cell samples at high concentration.

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Authors and Affiliations

  1. W. J. Schafer Associates, Inc., 1901 North Fort Myer Drive, 22209, Arlington, VA

    T. W. Barrett

  2. National Center for Small-Angle Scattering Research, Oak Ridge National Laboratory, 37830, Oak Ridge, Tennessee

    J. S. Lin

  3. Department of Biochemistry, University of Tennessee Center for the Health Sciences, 894 Union Avenue, 38163, Memphis, Tennessee

    L. M. Kraus

Authors
  1. T. W. Barrett
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  2. J. S. Lin
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  3. L. M. Kraus
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Barrett, T.W., Lin, J.S. & Kraus, L.M. X-Ray small-angle scattering from concentrated solutions of normal and sickle cell deoxyhemoglobin. J Biol Phys 15, 37–39 (1987). https://doi.org/10.1007/BF01867141

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  • DOI: https://doi.org/10.1007/BF01867141

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