Summary
The superselective transcatheter arterial embolization (TAE) and chemoembolization (TAC) of the periphery of the left and median lobes of the rat liver were performed using 1.5 mg of gelatin sponge dissolved in saline solution and mitomycin (MMC) at a dose of 1.6 mg/kg b.wt. The energy charge (EC) of the embolized and chemoembolized lobes decreased after the embolization but was restored 3 h later. The total hepatic blood flow (THBF) was reduced to about one half and required 1 week to be restored to the preembolization levels in both groups. Microscopic centrilobular necrosis and vacuolization were found. The addition of MMC did not produce marked untoward effects.
Thus, the normal liver is able to restore its energy pool despite a reduction in the THBF after superselective arterial embolization, and this recovery is not altered even by the use of a high dose of MMC.
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References
Adams H (1965) Adenosine-5′-diphosphate and adenosine-5′-monophosphate. In: Bergemeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York, pp 573–577
Atkinson DE (1968) The energy charge of adenylate pool as a regulatory parameter. Interaction with feedback modifiers. Biochemistry 7:4030–4034
Brown BW Jr, Hollander M (1977) Statistics: A biomedical introduction. John Wiley, New York London Sydney Toronto
Chuang BP, Wallace S (1980) Current status of transcatheter management of neoplasms. Cardiovasc Intervent Radiol 3:256–267
Cho KJ, Lunderquist A (1983) Experimental hepatic artery embolization with Gelfoam powder. Microfil perfusion study of the rabbit liver. Invest Radiol 18:189–193
Chuang VP, Wallace S (1981) Hepatic artery embolization in the treatment of hepatic neoplasms. Radiology 140:51–58
Donohoe MJ, Rush Br Jr, Maciedo GW, Barillo DJ, Murphy TF (1986) Biochemical and morphological changes in hepatocytes from the shock injured liver. Surg Gynecol Obstet 162:323–333
Doppman JL, Girton M, Kahn ER (1978) Proximal versus peripheral hepatic artery embolization: Experimental study in monkeys. Radiology 128:577–588
Hashimoto L, Ouchi K, Koyama K, Sato T (1985) Superselective arterial embolization of the rat liver. Tohoku J Exp Med 147:315–316
Hockerstedt K, Ahonen J, Nieminen J, Scheinin TM (1978) Transient liver hypoxia after liver hilus dearterialization. Ann Chir Gynecol 67:229–235
Jungerman K, Sasse D (1968) Heterogeneity of liver parenchymal cells. Trends Biochem Sci 3:198–202
Koshu K, Kamiyama K, Oka N, Endo S, Takaku A, Saito T (1982) Measurement of regional blood flow using hydrogen gas generated by electrolysis. Stroke 13:483–487
Lamprecht W, Trantschold I (1965) Determination with hexokinase and glucose-6-phosphate dehydrogenase. In: Bergemeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York, pp 543–551
Lemasters JJ, Stemkowski CJ, Ji S, Thurman RG (1983) Cell surface changes and enzyme release during hypoxia and reoxygenation in the isolated, perfused rat liver. J Cell Biol 97:778–786
Lutz J, Schulze HG (1978) Oxygen consumption and oxygen extraction of the feline liver under different types of induced hypoxia. In: Silver IA (ed) Oxygen transport to tissue, vol 3. Plenum Press, New York, pp 537–543
Nakao N, Miura K, Takayasu Y, Wada Y, Takahashi H, Hayashi H, Miura T (1984) Indication of transcatheter arterial embolization in hepatocellular carcinoma with liver cirrhosis evaluated from the liver function tests. Jpn J Gastroenterol 81:1995–1999 (in Japanese)
Ngala JF, Tanaka J, Ozawa K, Tobe T (1980) Stimulation of mitochondrial phosphorylative activity in the regenerating rabbit liver following oral antibiotic treatment. Eur Surg Res 12:87–94
Okamura J, Horikawa S, Fujiyama T, Mondem M, Kanbayashi J, Sikuhara O, Sakurai M, Kuroda C, Nakamura H, Kosagi G (1982) An appraisal of transcatheter arterial embolization combined with transcatheter arterial infusion of chemotherapeutic agents for hepatic malignancies. World J Surg 6:352–357
Ozawa K, Aoyama H, Yasuda K, Shimahara Y Nakatani T, Tanaka J, Yamamoto M, Kamiyama Y, Tobe T (1983) Metabolic abnormalities associated with postoperative organ failure. Arch Surg 118:1245–1251
Powell-Tuck J, McIvor J, Reynolds KW, Murray-Lyon IM (1984) Prediction of early death after therapeutic hepatic arterial embolization. Br Med J 288:1257–1259
Shimahara Y, Isselhard W (1986) Evaluation of a short-time, oxygen carrier-free perfusion model in rat liver: Mitochondrial energy metabolism and insulin effect. J Surg Res 40:69–76
Stridbeck H, Lorelius LE, Pirtle TE (1984) Development of collateral circulation following distal embolization of hepatic artery in pigs. Cardiovasc Intervent Radiol 7:240–244
Takahashi O, Miyazaki M, Fujimoto S, Endo F, Shimura T, Sugasawa H, Kurihara M, Okui K (1984) The effect of preoperative hepatic arterial embolization on regenerating liver after hepatic resection. Acta Hepatol Jpn 25:1005–1011 (in Japanese)
Taki Y, Tanaka J, Kamiyama Y, Takahashi J, Aoyama H, Ozawa K, Tobe T (1983) Significance of different response of high-energy metabolism to hepatic arterial embolization in normal and cirrhotic rat livers. In: Proceedings of the 18th Congress of the European Society for Surgical Research, p 92
Tango T (1983) Medical statistics. Asakusa, Tokyo (in Japanese)
Teicher BA, Lazo JS, Sartorelli AC (1981) Classification of antineoplastic agents by their selective toxicities toward oxygenated and hypoxic tumor cells. Cancer Res 41:73–81
Whittemore AD, Voorhes AB Jr, Price JB Jr (1976) Hepatic blood flow and pancreatic hormones as modifiers of hepatic regeneration. Surg Forum 27:363–365
Yamada R, Sato M, Kawabata M, Nakatsuka H, Nakamura K, Takashima S (1983) Hepatic artery embolization in 120 patients with unresectable hepatoma. Radiology 148:397–401
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Hashimoto, L., Ouchi, K., Fujiya, T. et al. Effects of superselective arterial embolization and chemoembolization on energy charge and total blood flow of the rat liver. Res. Exp. Med. 187, 329–337 (1987). https://doi.org/10.1007/BF01855658
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DOI: https://doi.org/10.1007/BF01855658