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Human melanoma-associated antigen expression on human neuroblastoma cells: Effects of differentiation inducers

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Summary

We have described two human melanoma-associated antigens (HMAA), recognized by the murine monoclonal antibodies LS62 and LS109. LS62 recognizes the neuroglandular antigen (NGA), which is overexpressed in neoplastic melanocytes as well as in several tissues of neuroectodermal origin. These antibodies were used to screen six neuroblastoma cell lines and one neuroepithelioma cell line. A melanoma cell line, G361, known to express the two antigens, was used as the positive control. Variable expression of the two antigens was detected in neuroblastoma cells. The surface expression of NGA and of the LS109 antigen was modulated in parallel with the morphological differentiation induced by retinoic acid, 5-bromodeoxyuridine, or cyclic AMP analog/activators. The modulation of the expression of the two HMAA was detected in G361 melanoma cells and in one of the neuroblastoma cell lines, SK-N-SH. These results suggest altered expression of both antigens during melanoma and neuroblastoma cell differentiation in culture.

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References

  1. Aubert C, Janiand P, Rouge F, Hanson C, Possman H, Rosengreen E (1980) Melanogenesis in cultured human neuroblastoma. Ann Clin Res 33: 2643

    Google Scholar 

  2. Biedler JL, Helson L, Spengler BA (1973) Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture. Cancer Res 33: 2643

    Google Scholar 

  3. Brodeur GM, Sekhon GS, Golstein MN (1977) Chromosomal aberrations in human neuroblastomas. Cancer 40: 2256

    Google Scholar 

  4. Cahan LD, Irie RF, Singh R, Cassidenti A, Paulson JC (1982) Identification of a human neuroectodermal tumor antigen (OFA-I-2) as ganglioside GD2. Proc Natl Acad Sci USA 79: 7629

    Google Scholar 

  5. Ciccarone V, Spengler BA, Meyers MB, Biedler JL, Ross R (1989) Phenotypic diversification in human neuroblastoma cells: expression of distinct neural crest lineages. Cancer Res 49: 219

    Google Scholar 

  6. De Chadarevian JP, Montes JL, O'Gorman AM, Freeman CR (1987) Maturation of cerebellar neuroblastoma into ganglioneuroma with melanosis. A histologic, immunochemical and ultrastructural study. Cancer 59: 59

    Google Scholar 

  7. Dehner LP (1986) Peripheral and central primitive neuroectodermal tumors. Arch Pathol Lab Med 110: 997

    Google Scholar 

  8. De Muralt B, De Tribolet N, Diserens AC, Stavron D, Mach JP, Carrel S (1985) Phenotyping of 60 cultured human gliomas and 34 other neuroectodermal tumors by means of monoclonal antibodies against glioma, melanoma and HLA-DR antigens. Eur J Cancer Clin Oncol 21: 207

    Google Scholar 

  9. Dixon WT, Demetrick DJ, Ohyama K, Sikora LKJ, Jerry LM (1990) Biosynthesis, glycosylation and processing of NGA, a human melanoma-associated antigen. Cancer Res 50: 4557

    Google Scholar 

  10. Dixon WT, Sikora LKJ, Demetrick DJ, Jerry LM (1990) Isolation and characterization of a heterodimeric surface antigen on human melanoma cells and evidence that it is the 4F2 cell activation/proliferation molecule. Int J Cancer 45: 59

    Google Scholar 

  11. Haynes BF, Hemmler ME, Mann DL, Eisenbarth GS, Shelhamer J, Mostowsky HS, Thomas CA, Strominger JL, Fauci AS (1981) Characterization of a monoclonal antibody (4F2). That binds to human monocytes and to a subset of activated lymphocytes. J Immunol 126: 1409

    Google Scholar 

  12. Honsik CJ, Jung G, Reisfeld RA (1986) Lymphokine-activated killer cells targeted by monoclonal antibodies to the disialogangliosides GD2 and GD3 specifically lyse human tumor cells of neuroectodermal origin. Proc Natl Acad Sci USA 83: 7893

    Google Scholar 

  13. Houghton AN, Eisinger M, Albino AP, Cairncross JG, Old L (1982) Surface antigens of melanocytes and melanomas. Markers of melanocyte differentiation and melanoma subsets. J Exp Med 156: 1755

    Google Scholar 

  14. Houghton AN, Real FX, Davis LJ, Cordon-Cardo C, Old LJ (1987) Phenotypic heterogeneity of melanoma. Relation to the differentiation program of melanoma cells. J Exp Med 164: 812

    Google Scholar 

  15. Hsu RO, Raine L, Fanger H (1981) The use of anti-avidin antibody and avidin-biotin-peroxidase complex in immunoperoxidase technique. Am J Clin Pathol 75: 816

    Google Scholar 

  16. Feyles V, Dixon WT, Sikora LKJ, Jerry LM (1989) Inhibition of Neuroglandular Antigen (NGA) glycosylation by phorbol ester in human melanoma cells. Biochem Biophys Res Commun 158: 837

    Google Scholar 

  17. Feyles V, Sikora LKJ, McGarry RC, Jerry LM (1990) Effects of retinoic acid and bromodeoxyuridine on human melanoma-associated antigen expression in small cell lung carcinoma cells. Oncology (in press)

  18. Gross N, Beck D, Favre S, Carrel S (1987) In vitro antigenic modulation of human neuroblastoma cells induced by IFN-, retinoic acid and bibutyryl cyclic AMP. Int J Cancer 39: 521

    Google Scholar 

  19. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 665

    Google Scholar 

  20. Laskey RA, Mills AD (1975) Quantitative film detection of3H and14C in polyacrylamide gels by fluorography. Eur J Biochem 56: 334

    Google Scholar 

  21. Letarte M, Quackenbush EJ, Baumal R, Michalak M (1986) Correlations between the 44D7 antigenic complex and the plasma membrane Na+-Ca2+ exchanger. Biochem Cell Biol 64: 1160

    Google Scholar 

  22. Liao S-K, Clarke BJ, Kwong PC, Brickenden A, Gallie BL, Dent PB (1981) Common neuroectodermal antigens on human melanoma, neuroblastoma, retinoblastoma, glioblastoma and fetal brain revealed by hybridoma antibodies raised against melanoma cells. Eur J Immunol 11: 450

    Google Scholar 

  23. Loken MR, Herzenberg LA (1975) Analysis of cell populations with a fluorescence-activated cell sorter. Ann NY Acad Sci 254: 163

    Google Scholar 

  24. Mullins JD (1980) A pigmented differentiating neuroblastoma. A light and ultrastructural study. Cancer 46: 522

    Google Scholar 

  25. Notter MFD, Leary JF (1987) Surface glycoproteins of differentiating neuroblastoma cells analyzed by lectin binding and flow cytometry. Cytometry 8: 518

    Google Scholar 

  26. Parks DR, Lanier LL, Herzenberg LA (1986) Flow cytometry and fluorescence activated cell sorting (FACS). In: Weir DM (ed) Handbook of experimental immunology, vol 1. Blackwell, Oxford, p 29.1

    Google Scholar 

  27. Reynolds CP, Maples J (1985) Modulation of cell surface antigens accompanies morphological differentiation of human neuroblastoma cell lines. In: Evans AE, D'Angio GJ, Seeger RC (eds) Advances in neuroblastoma research. Liss, New York, p 13

    Google Scholar 

  28. Reynolds CP, Biedler JL, Spengler BA, Reynolds DA, Ross RA, Frenkel EP, Smith RG (1986) Characterization of human neuroblastoma cell lines established before and after therapy. J Natl Cancer Inst 76: 375

    Google Scholar 

  29. Ross AR, Biedler JL (1985) Presence and regulation of tyrosinase activity in human neuroblastoma cell variants “in vitro” Cancer Res 37: 1364

    Google Scholar 

  30. Ross RA, Biedler JL (1985) Expression of a melanocyte phenotype in human neuroblastoma cells in vitro. In: Evans AE, D'Angio GJ, Seeger RC (eds) Advances in neuroblastoma research. Liss, New York, p 249

    Google Scholar 

  31. Seeger RC, Rayner SA, Banerjee A, Chung H, Laug WE, Neustein HB, Benedict WF (1977) Morphology, growth, chromosomal pattern, and fibrinolytic activity of two new human neuroblastoma cell lines. Cancer Res 37: 1364

    Google Scholar 

  32. Seeger RC, Rosenblatt HM, Imai K, Ferrone S (1981) Common antigenic determinants on human melanoma, glioma, neuroblastoma, and sarcoma cells defined with monoclonal antibodies. Cancer Res 41: 2714

    Google Scholar 

  33. Sikora LKJ, Pinto A, Demetrick DJ, Dixon WT, Urbanski SJ, Temple W, Jerry LM (1987) Characterization of a novel neuroglandular antigen (NGA) expressed on abnormal human melanocytes. Int J Cancer 39: 138

    Google Scholar 

  34. Sugimoto T, Kato T, Sawada T, Horii Y, Kemshead JT, Hino T, Morioka H, Hosoi H (1988) Schwann cell differentiation of human neuroblastoma cell lines in vitro induced by bromodeoxyuridine. Cancer Res 48: 2531

    Google Scholar 

  35. Tai T, Eisinger M, Ogata S, Old KO (1983) Glycoproteins as differentiation markers in human malignant melanoma and melanocytes. Cancer Res 43: 2773

    Google Scholar 

  36. Thomson TM, Real FX, Murakami S, Cordon-Cardo C, Old LJ, Houghton AN (1988) Differentiation antigens of melanocytes and melanoma: analysis of melanosome and cell surface markers of human pigmented cells with monoclonal antibodies. J Invest Dermatol 90: 459

    Google Scholar 

  37. Triche TJ (1986) Neuroblastoma — biology confronts nosology. Arch Pathol Lab Med 110: 994

    Google Scholar 

  38. Tsokos M, Scarpa S, Ross RA, Triche TJ (1987) Differentiation of human neuroblastoma recapitulates neural crest development. Am J Pathol 128: 484

    Google Scholar 

  39. Tumilowicz JJ, Nichols WW, Cholon JJ, Greene AE (1970) Definition of a continuous human cell line derived from neuroblastoma. Cancer Res 30: 2110

    Google Scholar 

  40. Wikstrand CJ, Bigner DD (1982) Expression of human fetal brain antigens by human tumors of neuroectodermal origin as defined by monoclonal antibodies. Cancer Res 42: 267

    Google Scholar 

  41. Ziegler-Heitbrock LHW, Munker R, Johnson J, Petersmann I, Schmoeckel C, Riethmuller G (1985) In vitro differentiation of human melanoma cells analyzed with monoclonal antibodies. Cancer Res 45: 1344

    Google Scholar 

  42. Zipf TF, Lauzon GJ, Longenecker BM (1983) A monoclonal antibody detecting a 39,000 M. W. molecule that is present on blood lymphocytes and chronic lymphocytic leukemia cells but is rare on acute lymphocytic leukemia blasts. J Immunol 131: 3064

    Google Scholar 

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Authors and Affiliations

  1. Oncology Research Group, The University of Calgary, Calgary, Alberta, Canada

    Valter Feyles, Walter T. Dixon, Lydia K. J. Sikora, Ronald C. McGarry & L. Martin Jerry

  2. Department of Pediatrics, The University of Calgary, Calgary, Alberta, Canada

    Ronald C. McGarry

  3. Department of Medicine, The University of Calgary, Calgary, Alberta, Canada

    L. Martin Jerry

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  1. Valter Feyles
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  2. Walter T. Dixon
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  3. Lydia K. J. Sikora
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  4. Ronald C. McGarry
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  5. L. Martin Jerry
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Feyles, V., Dixon, W.T., Sikora, L.K.J. et al. Human melanoma-associated antigen expression on human neuroblastoma cells: Effects of differentiation inducers. Cancer Immunol Immunother 32, 261–272 (1991). https://doi.org/10.1007/BF01789043

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