Abstract
The aim of this study was to determine whether tumor cells move actively through the linings of lymph node sinuses. Using 13762 carcinoma in F344 rats, and Walker carcinoma in Wistar rats, 20×106 tumor cells were injected into the footpad, and the ipsilateral popliteal lymph node examined by transmission electron microscopy. The same tumors were examined by making standard cell spots on plastic or glass surfaces, and examining these by phase and reflection contrast microscopy, fluorescent microscopy after anti-actin and phallacidin staining and transmission electron microscopy.
The 13762 cells do not migrate through the lining of the lymph node sinusoid, nor move activelyin vitro. Ultrastructural appearances of the Walker rat carcinoma cells suggest that they move actively through the sinus lining. After 24 hin vitro the Walker rat carcinoma cells in the centre of the spot are adherent to the surface. There is some movement of the edge of the sheet, and individual tumor cells at the edge of the sheet move actively and independently.
We conclude that the Walker rat carcinoma invades the lining of the lymph node sinusoid by active cell movement, and the 13762 carcinoma does not.
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Albrecht-Buehler, G., 1979, Group locomotion of PtKl cells.Experimental Cell Research,122, 402–407.
Albrecht-Buehler, G., 1976, Filopodia of spreading 3T3 cells. Do they have a substrate-exploring function?Journal of Cell Biology,69, 275–286.
Badley, R. A., Couchman, J. R., andRees, D. A., 1980, Comparison of the cell cytoskeleton in migratory and stationary chick fibroblasts.Journal of Muscle Research and Cell Motility,1, 5–14.
Barak, L. S., Yocum, R., Nothnagel, A., andWebb, W. W., 1980, Fluorescence staining of the actin cytoskeleton in living cells with 7-nitrobenz-2-oxa-1,3-diazolephallacidin.Proceedings of the National Academy of Science, U.S.A.,77, 980–984.
Bogden, A. E., Esber, H. J., Taylor, D. J., andGray, J. H., 1974, Comparative study on the effects of surgery, chemotherapy and immunotherapy, alone and in combination, on metastasis of the 13762 mammary adenocarcinoma.Cancer Research,34, 1627–1631.
Carr, I., 1983, Lymphatic Metastasis.Cancer Metastasis Reviews,1, 307–317.
Carr, I., andCarr, J., 1982, Experimental models of lymphatic metastasis.Tumor Invasion and Metastasis, edited by L. Liotta and I. Hart (Amsterdam: Nijhoff), pp. 189–205.
Carr, I., Carr, J., andDreher, B., 1981, Lymphatic metastasis of mammary adenocarcinoma.Invasion Metastasis,1, 34–53.
Carr, I., andMcGinty, F., 1974, Lymphatic metastasis and its inhibition; an experimental model.Journal of Pathology,113, 85–95.
Carr, I., McGinty, F., andNorris, P., 1976, The fine structure of neoplastic invasion: Invasion of liver, skeletal muscle and lymphatic vessels by the Rd/3 tumor.Journal of Pathology,118, 91–99.
Carr, J., Carr, I., Dreher, B., andBetts, K., 1980, Lymphatic metastasis: invasion of lymphatic vessels and efflux of tumour cells in the afferent popliteal lymph as seen in the Walker rat carcinoma.Journal of Pathology,132, 287–305.
Gabridge, M. G., 1981, The chamber/dish: an improved vessel for cell and expiant culture.In vitro 17, 91–97.
Haemmerli, G., 1981, Use of reflection contrast microscopy in the analysis of cellular motility.Virchows Archives (Cell Pathology),36, 35–40.
Haemmerli, G., Arnold, B., andStrauli, P., 1982, Cell locomotion, a continuing factor in spread of the V2 rabbit carcinoma.International Journal of Cancer,29, 223–227.
Haemmerli, G., andFelix, H., 1981, Interrelation of motility, shape, and surface architecture in human carcinoma cells.Cell Biology International Reports,5, 929–936.
Haemmerli, G., andFelix, H., 1982, Shape and motility, two interdependent features.Scanning Electron Microscopy,II, 731–739.
Haemmerli, G., andStrauli, P., 1981,In vitro motility of cells from human epidermoid carcinomas. A study by phase-contrast and reflection-contrast cinematography.International Journal of Cancer,27, 603–610.
Haemmerli, G., Strub, A. M., Jockusch, B. M., andStrauli, P., 1982, Filament patterns associated within vitro motility of human carcinoma cells.Cell Biology International Reports (London),6, 471–487.
Heath, J. P., andDunn, G. A., 1978, Cell to substratum contacts of chick fibroblasts and their relation to the microfilament system. A correlated interference-reflection and high-voltage electron-microscope study.Journal of Cell Science,29, 197–212.
Herman, I. M., Crisona, N. J., andPollard, T.D., 1981, Relation between cell activity and the distribution of cytoplasmic actin and myosin.Journal of Cell Biology,90, 84–91.
Izzard, C. S., andLochner, L. R., 1980, Formation of cell-to-substrate contacts during fibroblast motility: an interference-reflection study.Journal of Cell Science,42, 81–116.
Izzard, C. S., andLochner, L. R., 1976, Cell-to-substrate contacts in living fibroblasts: an interference reflection study with an evaluation of the technique.Journal of Cell Science,21, 129–159.
Jockusch, B. M., Haemmerli, G., andIn Albon, A., 1983, Cytoskeletal organization in locomoting cells of the V2 rabbit carcinoma.Experimental Cell Research,144, 251–263.
Kolega, J., 1981, The movement of cell clustersin vitro: morphology and directionality.Journal of Cell Science,49, 15–32.
Lazarides, E., 1975, Immunofluorescence studies on the structure of actin filaments in tissue culture cells.Journal of Histochemistry and Cytochemistry,23, 507–528.
Strauli, P., andHaemmerli, G., 1984, The role of cancer cell motility in invasion.Cancer Metastasis Reviews,3, 127–141.
Strauli, P., andWeiss, L., 1977, Cell locomotion and tumor penetration. Report on a workshop of the EORTC cell surface project group.European Journal of Cancer,13, 1–12.
Tosney, K. W., andWessells, N. K., 1983, Neuronal motility: the ultrastructure of veils and microspikes correlates with their motile activities.Journal of Cell Science,61, 389–411.
Verderame, M., Alcorta, D., Egnor, M., Smith, K., andPollack, R., 1980, Cytoskeletal F-actin patterns quantitated with fluorescein isothiocyanate-phalloidin in normal and transformed cells.Cell Biology,77, 6624–6628.
Volk, T., Geiger, B., andRaz, A., 1984, Motility and adhesive properties of high- and low-metastatic murine neoplastic cells.Cancer Research,44, 811–824.
Wallace, A. C., Josephson, R. L., andHollenberg, N. K., 1980, Observations on lymph node metastases.Metastatic Tumor Growth, edited by E. Grundmann (New York: Gustav Fischer Verlag), pp. 83–99.
Weiss, L., 1977,The Blood Cells and Hematopoietic Tissues (New York: McGraw-Hill, Inc.).
Yahara, I., Harada, F., Sekita, S., Yoshihira, K., andNatori, S., 1982, Correlation between effects of 24 different cytochalasins on cellular structures and cellular events and those on actinin vitro.Journal of Cell Biology,92, 69–78.
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Carr, I., Levy, M., Orr, K. et al. Lymph node metastasis and cell movement: ultrastructural studies on the rat 13762 mammary carcinoma and Walker carcinoma. Clin Exp Metast 3, 125–139 (1985). https://doi.org/10.1007/BF01758961
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DOI: https://doi.org/10.1007/BF01758961