Skip to main content
SpringerLink
Log in

Characterization of the third origin of DNA replication of the genome of insect iridescent virus type 6

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

The structure of the third origin of DNA replication (CIV-ori-M) of the genome (209 kbp) of Chilo iridescent virus (CIV) was determined by DNA nucleotide sequence analysis. The CIV-ori-M is located within the DNA sequences of theEcoRI CIV DNA fragment M (7 kbp; 0.310–0.345 viral map units) between the genome coordinates 0.310 (EcoRI site) and 0.317 (NcoI site). The DNA nucleotide sequence of theEcoRI/NcoI CIV DNA fragment (1601 bp) was determined for identifying the DNA sequence of the corresponding origin of DNA replication. The analysis of the DNA sequences of this region revealed the presence of a 12-mer inverted repeat at nucleotide positions 485–496 and 503–513 (485-AGATATTTGACT-496-TATGT-503-AGTCAAATATCT-513) that are able to form a hairpin-loop structure. A double-stranded DNA fragment was synthesized that corresponds to the nucleotide positions 485–513 that were cloned into the phages M13mp18 and M13mp19, and were screened for their ability to be amplified in CF-124 cell cultures infected with CIV. The successful amplification of the DNA sequence of the CIV-ori-M is strong evidence that this particular region of the CIV genome indeed serves as the origin of DNA replication.

The analysis of the DNA sequence of CIV-ori-M in comparison to the DNA sequence of the two other characterized origins of DNA replication (CIV-ori-H and Y) of the CIV genome (9) was carried out, and the results are shown in Table 2. According to these data the DNA sequence homology between the DNA sequences of the CIV-ori-M and CIV-ori-Y, and between the CIV-ori-M or CIV-ori-H, was found to be 58% and 55%, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Fischer M., Schnitzler P., Delius H., Rösen-Wolff A. and Darai G. in Darai G. (ed).Molecular Biology of Iridoviruses. Kluwer Academic Publishers, Boston, 1990, pp. 47–80.

    Google Scholar 

  2. Schnitzler P., Soltau J.B., Fischer M., Reisner H., Scholz J., Delius H. and Darai G., Virology160 66–74, 1987.

    Google Scholar 

  3. Soltau J.B., Fischer M., Schnitzler P., Scholz J. and Darai, G., J Gen Virol68 2717–2722, 1987.

    Google Scholar 

  4. Fukuya M. and Nasu S., Appl Entomol Zool1 69–72, 1966.

    Google Scholar 

  5. Smith, K.M. (ed), inVirus-insect relationships, Longman, London, 1976, pp. 102–104.

    Google Scholar 

  6. Jensen D.D., Hukutsara T. and Tanada Y., J Invert Pathol19 276–278, 1972.

    Google Scholar 

  7. Ohba M. and Aizawa K., J Invert Pathol33 278–283, 1979.

    Google Scholar 

  8. Stow N.D. and McMonagle E.C., Virology130 427–438, 1983.

    Google Scholar 

  9. Handermann M., Schnitzler P., Rosen-Wolff A., Raab K., Sonntag K.C. and Darai G., Virus Genes, in press, 1991.

  10. Fischer M., Schnitzler P., Delius H. and Darai G., Virology167 4485–4496, 1988.

    Google Scholar 

  11. Southern E.M., J Mol Biol98 503, 1975.

    Google Scholar 

  12. Rigby P.W.J., Dieckman M., Rhodes C. and Berg P., J Mol Biol114 237–256, 1977.

    Google Scholar 

  13. Yanisch-Perron C., Vieira J. and Messing J., Gene33 103–119, 1985.

    Google Scholar 

  14. Sanger F., Nicklen S. and Coulson A.R., Proc Natl Acad Sci USA74 5463–5467, 1977.

    Google Scholar 

  15. Sanger F. and Coulson A.R., FEBS Lett87 107–110, 1978.

    Google Scholar 

  16. Vieira J. and Messing J., Gene19 259–268, 1982.

    Google Scholar 

  17. Tabor S. and Richardson C., Proc Natl Acad Sci USA74 4767–4771, 1987.

    Google Scholar 

  18. Fischer M., Schnitzler P., Scholz J., Rösen-Wolff A., Delius H. and Darai G., Virology167 497–506, 1988.

    Google Scholar 

  19. Goorha R. and Murti K.G., Proc Natl Acad Sci USA79 152–248, 1982.

    Google Scholar 

  20. Darai G., Anders K., Koch H.G., Delius H., Gelderblom H., Samalecos C. and Flügel R.M., Virology126 466–479, 1983.

    Google Scholar 

  21. Delius H., Darai G. and Flügel R.M., J Virol49 609–614, 1984.

    Google Scholar 

  22. Ward V.K. and Kalmakoff J., Virology160 507–510, 1987.

    Google Scholar 

  23. Kascsak R.J., Rubestein R., Merz P.A., Carp R.I., Wisnieski H.M. and Diringer H., J Gen Virol66 1715–1722, 1985.

    Google Scholar 

  24. Chirgwin J.M., Przybyla A.E., MacDonald R.J. and Rutter W.J., Biochemistry18 5294–5296, 1979.

    Google Scholar 

  25. Chomczynski P. and Sacchi N., Anal Biochem162 156–169, 1987.

    Google Scholar 

  26. Watson C.J. and Jackson J.F. in Glover, D.M. (ed). (1985)DNA cloning Vol. I, IRL Press, Oxford, 1985, pp. 79–88.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Medizinische Virologie der Universitat Heidelberg, Im Neuenheimer Feld 324, 6900, Heidelberg, Germany

    Kai-Christian Sonntag & Gholamreza Darai

Authors
  1. Kai-Christian Sonntag
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gholamreza Darai
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sonntag, KC., Darai, G. Characterization of the third origin of DNA replication of the genome of insect iridescent virus type 6. Virus Genes 6, 333–342 (1992). https://doi.org/10.1007/BF01703082

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01703082

Key words

Search

Navigation