Abstract
The complete nucleotide sequence of genome segment 11 from the noncultivatable, human group C rotavirus (Bristol strain) was determined. Comparison of the nucleotide sequence of the segment termini with the consensus 5′ and 3′ terminal noncoding sequences of the human group C rotavirus genome revealed characteristic 5′ and 3′ sequences. Human group C rotavirus genome segment 11 is 613 bp long and encodes a single open reading frame of 450 nucleotides (150 amino acids) starting at nucleotide 39 and terminating at nucleotide 489, leaving a long 3′ untranslated region of 124 nucleotides. The predicted translation product has a calculated molecular weight of 17.7 kD and contains four potential N-linked glycosylation sites. No significant homologies to other viral proteins were found in database searches. Hydropathy analysis predicted the human group C rotavirus genome segment 11 translation product has a hydrophilic carboxy terminus (amino acids 54–150) and a hydrophobic amino terminus (amino acids 1–53) that can be further subdivided into three short hydrophobic sequences—H1, H2, and H3. These features are analogous to the integral membrane glycoprotein NSP4 encoded by group A rotavirus gene 10.
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Deng, Y., Fielding, P.A., Lambden, P.R. et al. Molecular characterization of the 11th RNA segment from human group C rotavirus. Virus Genes 10, 239–243 (1995). https://doi.org/10.1007/BF01701813
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DOI: https://doi.org/10.1007/BF01701813