Abstract
Cotyledons cut from axenic seedlings were immersed inAgrobacterium tumefaciens suspension which was treated with acetosyringone and nopaline at low pH overnight. The infected cotyledon explants were cultured on MSB medium (MS salts + B5 Vitamins) containing 6-BA 3mg/1 for 2–3 days, and transferred onto selective medium (MSB with kanamycin 50–100 mg/l). Kanamycin-resistant shoots were selected. More than 60 regenerated plants were obtained. About 60% of the plants showed high NPT II activity. Southern blot hybridization showed that some of the plants gave a positive signal with the insecticidal crystal protein gene (cry IA gene) probe, and exhibited tolerant to insects such asPieris rapae (cabbage caterpillar) in leaf feeding experiments. Kanamycin-resistance and insect-resistance were maintained in the progeny.
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Abbreviations
- 6-BA:
-
6-benzylaminopurine
- IBA:
-
indole-3-butyric acid
- CryIA gene :
-
bacillus thuringiensis insecticidal crystal protein genecryIA
- NPT II:
-
neomycin phosphotransferase II
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Communicated by J. M. Widholm
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Li, Xb., Mao, HZ. & Bai, YY. Transgenic plants of rutabaga (Brassica napobrassica) tolerant to pest insects. Plant Cell Reports 15, 97–101 (1995). https://doi.org/10.1007/BF01690262
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DOI: https://doi.org/10.1007/BF01690262