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Serum antibodies against common antigens of bacterial lipopolysaccharides in healthy adults and in patients with multiple myeloma

Serum-Antikörper gegen Gruppenantigene bakterieller Lipopolysaccharide bei gesunden Erwachsenen und bei Patienten mit multiplem Myelom

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Summary

The incidence of infections caused by gramnegative bacteria is increased in patients with multiple myeloma due to secondary humoral immunodeficiency. In order to diagnose patients with increased susceptibility to gram-negative infections, serum antibodies against common determinants of lipopolysaccharides (lipid A and core-polysaccharide) were determined by a rapid enzyme-linked immunosorbent assay (ELISA). It was possible to define a group of patients at high risk of contracting gram-negative infections using this test. Intravenous IgG preparations used as a substitute were shown to contain antibodies against these common antigens. However, it is suggested that the clinically recognized efficacy of these preparations could be due to their containing anti-LPS antibodies.

Zusammenfassung

Aufgrund ihres sekundären humoralen Immundefektes treten bei Patienten mit multiplem Myelom gehäuft Infektionen durch gramnegative Bakterien auf. Um Patienten mit einer erhöhten Anfälligkeit für gramnegative Infektionen zu diagnostizieren, wurden die Serum-Antikörper gegen gemeinsame Determinanten der Lipopolysaccharide (Lipid A und zentrales Polysaccharid) in einem schnellen enzymverbundenen Immunbindungstest (ELISA) bestimmt. Mit diesem Test-system war es möglich, eine Gruppe von Patienten mit einem hohen Risiko für gramnegative Infektionen zu definieren. Es wurde gezeigt, daß IgG-Präparationen, die zur intravenösen Substitution verwendet werden, Antikörper gegen diese gemeinsamen Antigene enthalten. Dabei ist zu vermuten, daß die klinisch gefundene Wirksamkeit dieser Präparationen auf ihrem Gehalt an anti-LPS-Antikörpern beruhen könnte.

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Authors and Affiliations

  1. Abteilung Immunologie und Transfusionsmedizin, Zentrum Innere Medizin und Dermatologie, Medizinische Hochschule Hannover, Konstanty-Gutschow-Str. 8, D-3000, Hannover 61

    C. Stoll,  I. Schedel &  D. Peest

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  1. C. Stoll
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  2. I. Schedel
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  3. D. Peest
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Stoll, C., Schedel, I. & Peest, D. Serum antibodies against common antigens of bacterial lipopolysaccharides in healthy adults and in patients with multiple myeloma. Infection 13, 115–119 (1985). https://doi.org/10.1007/BF01642869

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