References
Bain B, Catovsky D, O'Brien M, Spiers ASD, Richards HGH (1977) Megakaryoblastic transformation of chronic granulocytic leukaemia. An electron microscopy and cytochemical study. J Clin Pathol 30:235–242
Bartl R, Frisch B, Burkhardt R (1982) Structure and function of the normal bone marrow. In: Bartl R, Frisch B, Burkhardt R (eds) Bone marrow biopsies revisited. A new dimension for hematological malignancies. Karger, Basel, pp 8–14
Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DAG, Gralnick HR, Sultan C (1982) Proposals for the classification of the myelodysplastic syndromes. Br J Haematol 51:189–199
Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DAG, Gralnick HR (1985) Criteria for the diagnosis of acute leukemia of megakaryocyte lineage (M7): a report of the French-Ameri-can-British Cooperative Group. Ann Intern Med 103:460–562
Brannan DP, Guthrie TH (1988) Idiopathic thrombocytopenic purpura in adults. South Med J 81:75–80
Breton-Gorius J (1981) On the alleged phagocytosis by megakaryocytes. Br J Haematol 47:635–636
Breton-Gorius J, Vainchenker W (1986a) Expression of platelet proteins during the in-vitro and in-vivo differentiation of megakaryocytes and morphological aspects of their maturation. Semin Hematol 1:43–67
Breton-Gorius J, Vainchenker W (1986b) Immunological and cytochemical characterization of megakaryocytic lineage leukemia. Prog Clin Biol Res 215:301–317
Breton-Gorius J, Tabilio A, Vainchenker W, Vinci G, Van Haeke D, Henri A, Guichard J, Canizo M (1984) Diagnosis of megakaryoblastic leukaemias. In: Lennert K, Hübner K (eds) Pathology of the bone marrow. Fischer, Stuttgart, pp 114–129
Breton-Gorius J, Villeval JL, Kieffer N, Mitjavila MT, Guichard J, Vainchenker W (1989) Limits of phenotypic markers for the diagnosis of megakaryoblastic leukemia. Blood Cells 15:259–277
Branehög I, Ridell B, Swolin B, Weinfeld A (1975) Megakaryocyte quantifications in relation to thrombokinetics in primary thrombocythaemia and allied diseases. Scand J Haematol 15:321–332
Burkhardt R (1988) Bone marrow in megakaryocytic disorders. Hematol Oncol Clin North Am 2:695–733
Burkhardt R, Frisch B, Bartl R (1982) Bone biopsy in haematological disorders. J Clin Pathol 35:257–284
Burkhardt R, Kleinknecht R, Jäger K, Frisch B, Mahl G, Bartl R (1984a) Megakaryocytic emperipolesis — accidental or diagnostic sign? In: Lennert K, Hübner K (eds) Pathology of the bone marrow. Fischer, Stuttgart, pp 200–205
Burkhardt R, Bartl R, Jäger K, Frisch B, Kettner B, Mahl G, Sund M (1984b) Chronic myeloproliferative disorders (CMPD). Pathol Res Pract 1:131–186
Burkhardt R, Bartl R, Jäger K, Frisch B, Kettner B, Mahl G, Sund M (1986) Working classification of chronic myeloproliferative disorders based on histological, haematological and clinical findings. J Clin Pathol 39:237–252
Burkhardt R, Jaeger K, Kettner G, Helmer G (1990) Chronic myeloproliferative disorders: prognostic importance of new working classification. J Clin Pathol 43:357–364
Castro-Malaspina H (1984) Pathogenesis of myelofibrosis: role of ineffective megakaryopoiesis and megakaryocyte components. In: Berk P, Castro-Malaspino H, Wasserman L (eds) Myelofibrosis and the biology of connective tissue. Liss, New York, pp 427–454
De Alarcon PA, Mazur EM, Schmieder JA (1987) In vitro megakaryocytopoiesis in children with acute idiopathic thrombocytopenic purpura. Am J Pediatr Hematol Oncol 9:212–218
Delacretaz F, Schmidt P-M, Piguet D, Bachmann F, Costa J (1987) Histopathology of myelodysplastic syndromes. The FAB classification (proposals) applied to bone marrow biopsy. Am J Clin Pathol 87:180–186
De Oliveira MSP, Gregory C, Matutes E, Parreira A, Catovsky D (1987) Cytochemical profile of megakaryoblastic leukaemia: a study with cytochemical methods, monoclonal antibodies, and ultrastructural cytochemistry. J Clin Pathol 40:663–669
Eldor A, Vlodavsky I, Deutsch V, Levine RF (1989) Megakaryocyte function and dysfunction. Bailliere's Clin Haematol 2:543–568
Ellis JT, Peterson P (1979) The bone marrow in polycythemia vera. Pathol Ann 14:383–403
Ellis JT, Silver RT, Coleman M, Geller SA (1975) The bone marrow in polycythemia vera. Semin Hematol 12:433–444
Erber WN, Jacobs A, Oscier DG, O'Hea AM, Mason DY (1987) Circulating micromegakaryocytes in myelodysplasia. J Clin Pathol 40:1349–1352
Fohlmeister I, Fischer R, Mödder B, Rister M, Schaefer H-E (1985a) Aplastic anaemia and the hypocellular myelodysplastic syndrome: histomorphological, diagnostic, and prognostic features. J Clin Pathol 38:1218–1224
Fohlmeister I, Fischer R, Schaefer H-E (1985b) Preleukemic myelodysplastic syndromes (MDS): pathogenetical considerations based on retrospective clinicomorphological sequential studies. Anticancer Res 5:179–188
Fox SB, Lorenzen J, Heryet A, Jones M, Gatter KC, Mason DY (1990) Megakaryocytes in myelodysplasia: an immunohistochemical study on bone marrow trephines. Histopathology 17:69–74
Frisch B, Bartl R (1985) Histology of myelofibrosis and osteomyelosclerosis. In: Lewis SM (ed) Myelofibrosis, pathophysiology and clinical management. Dekker, New York, pp 51–86
Frisch B, Bartl R (1986) Bone marrow histology in myelodysplastic syndromes. Scand J Haematol 36:21–37
Frisch B, Bartl R, Burkhardt R (1982) Bone marrow biopsy in clinical medicine: an overview. Haematologia 3:245–285
Frisch B, Bartl R, Burkhardt R, Jäger K, Mahl G, Sund G, Kettner G (1984) Classification of myeloproliferative disorders by bone marrow histology. Bibl Haematol (Basel) 50:57–80
Frisch B, Lewis SM, Burkhardt R, Bartl R (1985) Biopsy pathology of bone and bone marrow. Chapman and Hall, London
Gatter KC, Cordell JL, Turley H, Heryet A, Kieffer N, Anstee DJ, Mason DY (1988) The immunohistological detection of platelets, megakaryocytes and thrombi in routinely processed specimens. Histopathology 13:257–267
Georgii A, Vykoupil KF, Thiele J (1984a) Classification of chronic myeloproliferative diseases by bone marrow biopsies. Haematological and cytogenetic findings and clinical course. Bibl Haematol (Basel) 50:41–56
Georgii A, Vykoupil KF, Thiele J (1984b) Histopathology of bone marrow and clinical findings in chronic myeloproliferative disorders. In: Lennert K, Hübner K (eds) Pathology of the bone marrow. Fischer, Stuttgart, pp 147–169
Georgii A, Vykoupil KF, Buhr T (1987) Preleukemia: bone marrow histopathology in myelodysplasia and preleukemic syndrome. Recent Results Cancer Res 106:159–171
Georgii A, Vykoupil KF, Buhr T, Choritz H, Döhler U, Kaloutsi V, Werner M (1990) Chronic myeloproliferative disorders in bone marrow biopsies. Pathol Res Pract 186:3–27
Gewirtz AM (1986) Human megakaryocytopoiesis. Semin Hematol 23:27–42
Halil O, Barrett AJ (1980) Phagocytosis by megakaryocytes in malignant disorders. Br J Haematol 46:161
Harker LA (1968) Megakaryocyte quantitation. J Clin Invest 47:452–457
Harker LA (1970) Thrombokinetics in idiopathic thrombocytopenic purpura. Br J Haematol 19:95–104
Hoffman R (1989) Regulation of megakaryocytopoiesis. Blood 74:1196–1212
Hoffman R, Straneva J, Yang HH, Bruno E, Brandt J (1987) New insights into the regulation of human megakaryocytopoiesis. Blood Cells 13:75–86
Huang M, Li H-Y, Nichols WL, Young J-H, Katzman JA (1984) Acute leukemia with megakaryocytic differentiation: a study of 12 cases identified immunocytochemically. Blood 64:427–439
Iland H, Laszlo J, Peterson P, Murphy S, Briere J, Weinfeld A, Rosenthal DS, Landaw SA, Ellis JT, Silverstein MN, Wasserman LR (1983) Essential thrombocythemia: clinical and laboratory characteristics at presentation. Trans Assoc Am Phys 96:165–174
Iland HJ, Laszlo J, Case DC, Murphy S, Reichert TA, Tso CY, Wasserman LR (1987) Differentiation between essential thrombocythemia and polycythemia vera with marked thrombocytosis. Am J Hematol 25:191–201
Ishibashi T, Ruggeri ZM, Harker LA, Burstein SA (1986) Separation of human megakaryocytes by state of differentiation on continuous gradients of percoll: size and ploidy analysis of cells identified by monoclonal antibody to glycoprotein IIb/IIIa. Blood 67:1286–1292
Jacobs A (1985) Myelodysplastic syndromes: pathogenesis, functional abnormalities, and clinical implications. J Clin Pathol 38:1201–1217
Jacobs P, LeRoux I, Jacobs L (1984) Megakaryoblastic transformation in myeloproliferative disorders. Cancer 54:297–302
Juvonen E, Partanen S, Knuutila S, Ruutu T (1986) Megakaryocyte colony formation by bone marrow progenitors in myelodysplastic syndromes. Br J Haematol 63:331–334
Kanz L, Löhr GW, Fauser AA (1987) Human megakaryocytic progenitor cells. Klin Wochenschr 65:297–307
Kitagawa M, Kamiyama R, Takemura T, Kasuga T (1989) Bone marrow analysis of the myelodysplastic syndromes: histological and immunohistochemical features related to the evolution of overt leukemia. Virchows Archiv [B] 57:47–53
Knecht H, Streuli RA (1985) Megakaryocytopoiesis in different forms of thrombocytosis and thrombocytopenia: identification of megakaryocyte precursors by immunostaining of intracytoplasmic factor-VIII-related antigen. Acta Haematol (Basel) 74:208–212
Koike T (1984) Megakaryoblastic leukemia: the characterization and identification of megakaryoblasts. Blood 64:683–692
Kutti J, Ridell B, Weinfeld A (1973) The relation of thrombokinetics to bone marrow megakaryocytes and to the size of the spleen in polycythaemia vera. Scand J Haematol 10:88–95
Larsen TE (1970) Emperipolesis of granular leukocytes within megakaryocytes in human hematopoietic bone marrow. Am J Clin Pathol 53:485–489
Lazzarino M, Morra E, Castello A, Inverardi D, Coci A, Pagnucco G, Magrini U, Zei G, Bernasconi C (1986) Myelofibrosis in chronic granulocytic leukaemia: clinopathologic correlations and prognostic significance. Br J Haematol 64:227–240
Levene RB, Lamaziere JMD, Broxmeyer HE, Lu L, Rabellino EM (1985) Human megakaryocytes: V. Changes in the phenotypic profile of differentiating megakaryocytes. J Exp Med 161:457–474
Levene RB, Williams NT, Lamaziere JMD, Rabellino EM (1987) Human megakaryocytes. IV. Growth and characterization of clonable megakaryocyte progenitors in agar. Exp Hematol 15:181–189
Levine RF, Hazzard KC, Lamberg JD (1982) The significance of megakaryocyte size. Blood 60:1122–1131
Lichtman MA, Chamberlain JK, Simon W, Santillo PA (1978) Parasinusoidal location of megakaryocytes in marrow: a determinant of platelet release. Am J Hematol 4:303–312
Lorand-Metze I (1989) Bone marrow morphology at diagnosis as a prognostic parameter in Philadelphia-positive chronic myelogenous leukaemia (CML). Br J Haematol 71:163
Lorand-Metze I, Vassallo J, Carmino AS (1987) Histological and cytological heterogenity of bone marrow in Philadelphia-positive chronic myelogenous leukaemia at diagnosis. Br J Haematol 67:45–49
Lundin PM, Ridell B, Weinfeld A (1972) The significance of bone marrow morphology for the diagnosis of polycythaemia vera. Scand J Haematol 9:271–282
MacCarthy DM (1985) Fibrosis of the bone marrow: content and causes. Br J Haematol 59:1–7
Mazur EM (1987) Megakaryocytopoiesis and platelet production: a review. Exp Hematol 15:340–350
Mazur EM, Hoffman R, Chasis J, Marchesis S, Bruno E (1981) Immunofluorescent identification of human megakaryocyte colonies using an antiplatelet glycoprotein antiserum. Blood 2:277–286
Mazur EM, Lindquist DL, De Alarcon PA, Cohen JL (1988) Evaluation of bone marrow megakaryocyte ploidy distributions in persons with normal and abnormal platelet counts. J Lab Clin Med 111:194–202
McDonald TP (1989) The regulation of megakaryocyte and platelet production. Int J Cell Cloning 7:139–155
Murphy S (1983) Thrombocytosis and thrombocythemia. Clin Hematol 12:89–106
Murphy S, Iland H, Rosenthal D, Laszlo J (1986) Essential thrombocythemia: an interim report from the Polycythemia Vera Study Group. Semin Hematol 23:177–182
Parmley RT, Kim TH, Austin RL, Alvarado CS, Ragab AH (1982) Emperipolesis of neutrophils by dysmorphic megakaryocytes. Am J Hematol 13:303–311
Polli N, O'Brien M, Tavares De Castro J, Matutes E, San Miguel JF, Catovsky D (1985) Characterization of blast cells in chronic granulocytic leukaemia in transformation, acute myelofibrosis and undifferentiated leukaemia. Br J Haematol 59:227–296
Queisser U, Queisser W, Spiertz B (1971) Polyploidization of megakaryocytes in normal humans, in patients with idiopathic thrombocytopenia and with pernicious anaemia. Br J Haematol 20:489–501
Rabellino EM, Levene RB, Leung LLK, Nachman RL (1981) Human megakaryocytes. II. Expression of platelet proteins in early marrow megakaryocytes. J Exp Med 154:88–100
Renner D, Propp H, Queisser W (1987) Diploid and tetraploid precursors of megakaryocytes in normal human bone marrow detected by immunofluorescence. Blut 55:459–466
Rozman C, Cervantes F, Feliu E (1989) Is the histological classification of chronic granulocytic leukaemia justified from the clinical point of view? Eur J Haematol 42:150–154
Ruiz-Argüelles GJ, Marin-Lopez A, Lobato-Mendizabal E, Ruiz-Arguelles A, Nichols WL, Katzman JA (1986) Acute megakaryoblastic leukaemia: a prospective study of its identification and treatment. Br J Haematol 62:55–63
San Miguel JF, Tavares De Castro J, Matutes E, Rodriguez B, Poli N, Zola H, McMichael AJ, Bollum FJ, Thompson DS, Goldman JM, Catovsky D (1985) Characterization of blast cells in chronic granulocytic leukaemia in transformation, acute myelofibrosis and undifferentiated leukaemia. II. Studies with monoclonal antibodies and terminal transferase. Br J Haematol 59:297–309
San Miguel JF, Gonzales M, Canizo MC, Ojeda E, Orfao A, Caballero MD, Moro MJ, Fisac P, Borrasca AL (1988) Leukemias with megakaryoblastic involvement: clinical, hematologic, and immunologic characteristics. Blood 72:402–407
Sims RB, Gewirtz AM (1989) Human megakaryocytopoiesis. Annu Rev Med 40:213–224
Tabilio A, Vainchenker W, Van Haeke D, Vinci G, Guichard J, Henri A, Reyes F, Breton-Gorius F (1984) Immunological characterization of the leukaemic megakaryocytic line at light and electron microscopic levels. Leuk Res 8:769–781
Tavassoli M, Aoki M (1989) Localization of megakaryocytes in the bone marrow. Blood Cells 15:3–14
Thiele J, Vykoupil K-F, Georgii A (1980) Myeloid dysplasia (MD): a hematological disorder preceding acute and chronic myeloid leukemia. Virchows Arch [A] 389:343–367
Thiele J, Holgado S, Choritz H, Georgii A (1982) Abnormalities of megakaryocytes in myelitis and chronic myeloproliferative diseases. Virchows Arch [B] 41:67–82
Thiele J, Holgado S, Choritz H, Georgii A (1983) Density distribuion and size of megakaryocytes in inflammatory reactions of the bone marrow (myelitis) and chronic myeloproliferative diseases. Scand J Haematol 31:329–341
Thiele J, Krech R, Choritz H, Georgii A (1984a) Emperipolesis — a peculiar feature of megakaryocytes as evaluated in chronic myeloproliferative diseases by morphometry and ultrastructure. Virchows Arch [B] 46:253–263
Thiele J, Funke S, Holgado S, Choritz H, Georgii A (1984b) Megakaryopoiesis in chronic myeloproliferative diseases: a morphometrical evaluation with special emphasis on primary thrombocythemia. Analyt Quant Cytol Histol 6:155–167
Thiele J, Schneider G, Hoeppner B, Wienhold S, Zankovich R, Fischer R (1988a) Histomorphometry of bone marrow biopsies in chronic myeloproliferative disorders with associated thrombocytosis — features of significance for the diagnosis of primary (essential) thrombocythaemia. Virchows Arch [A] Anat 413:407–417
Thiele J, Höppner B, Zankovich R, Fischer R (1989) Histomorphometry of bone marrow biopsies in primary osteomyelofibrosis /-sclerosis (so-called agnogenic myeloid metaplasia) — correlations between laboratory and morphological features. Virchows Arch [A] 415:191–202
Thiele J, Wienhold S, Zankovich R, Fischer R (1990a) A histomorphometric analysis of trephine biopsies of bone marrow from 65 patients with chronic myeloid leukemia. Classification of patients into subgroups with different survival patterns. Analyt Quant Cytol Histol 12:103–116
Thiele J, Wagner S, Dienemann D, Wienhold S, Fischer R, Stein H (1990b) Megakaryocyte precursors (pro- and megakaryoblasts) within the normal human bone marrow. An immunohistochemical and morphometric study on routinely processed trephine biopsies. Analyt Quant Cytol Histol 12:285–289
Thiele J, Wagner S, Dienemann D, Wienhold S, Fischer R, Stein H (1990c) An immunomorphometric study on megakaryocyte precursor cells in bone marrow tissue from patients with chronic myeloid leukemia (CML). Eur J Haematol 44:63–70
Travis WD, Chin-Yang Li, Banks PM, Nichols WL (1987) Megakaryoblastic transformation of chronic granulocytic leukemia. Cancer 60:193–200
Tricot G, De Wolf-Peeters C, Hendricks B, Verwilghen RL (1984) Bone marrow histology in myelodysplastic syndromes. I. Histological findings in myelodysplastic syndromes and comparison with bone marrow smears. Br J Haematol 57:423–430
Vainchenker W, Deschamps JF, Bastin JM, Guichard J, Titeux M, Breton-Gorius J, McMichael AJ (1982) Two monoclonal antibodies as markers of human megakaryocyte maturation: immunofluorescent staining and peroxidase detection in megakaryocyte colonies and in in vivo cells from normal and leukemic patients. Blood 59:514–521
Velez-Garcia EO, Fradera J, DeLourdes Telmont M, White JG (1985) Megakaryoblastic transformation of Ph-positive chronic granulocytic leukemia. Am J Clin Pathol 2:228–233
Vinci G, Tabilio A, Deschamps JF, Van Haeke D, Henri A, Guichard J, Tetteroo P, Landsdorp PM, Hercend T, Vainchenker W, Breton-Gorius J (1984) Immunological study of in vitro maturation of human megakaryocytes. Br J Haematol 56:589–605
Williams N, Levine RF (1982) The origin, development and regulation of megakaryocytes. Br J Haematol 52:173–180
Wisneth M, Pflieger H, Kubaneck B, Heimpel H (1980) Micromegakaryocytes in human bone marrow. Acta Haematol (Basel) 64:65–71
Wolf BC, Neiman RS (1985) Myelofibrosis with myeloid metaplasia: pathophysiologic implications of the correlation between bone marrow changes and progression of splenomegaly. Blood 65:803–809
Yoshida Y, Oguma S, Uchino H, Maekawa T (1988) Refractory myelodysplastic anaemias with hypocellular bone marrow. J Clin Pathol 41:763–767
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Thiele, J., Fischer, R. Megakaryocytopoiesis in haematological disorders: Diagnostic features of bone marrow biopsies. Vichows Archiv A Pathol Anat 418, 87–97 (1991). https://doi.org/10.1007/BF01600283
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DOI: https://doi.org/10.1007/BF01600283