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Phagocytic inhibitory activity in serum of cats immunized withPseudomonas aeruginosa lipopolysaccharide

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Abstract

Sequentially collected sera from cats chronically immunized withPseudomonas aeruginosa lipopolysaccharide (LPS) serotype 5 were assessed for their effect on phagocytosis by alveolar macrophages from nonimmunized cats. Phagocytosis was measured by incubating macrophage monolayers for 20 min in the presence of3H-labeled bacteria and 5% serum from control or immunized animals. Sustained phagocytic inhibitory activity developed in the sera of eight of 11 immunized cats (mean inhibition ranged from 30% to 73%) after 13 weekly immunizations. The activity was specific because phagocytosis ofStaphylococcus aureus andP. aeruginosa of another serotype (type 6) was unimpaired. An enzyme-linked immunosorbent assay showed an increase in the amount of LPS-specific IgG in postimmunization sera from the eight cats with inhibitory activity. The IgG appeared to be serotype specific because significantly higher titers were obtained against LPS serotype 5 than LPS serotype 6. The results suggest that phagocytic inhibitory activity in sera from LPS-immunized cats may be due to anti-LPS IgG.

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Author notes

  1. Jeffrey D. Klinger

    Present address: Integrated Genetics, 01701, Framingham, MA, USA

  2. Mary Jane Thomassen

    Present address: Department of Pulmonary Diseases, Cleveland Clinic Foundation, 44106, Cleveland, OH, USA

Authors and Affiliations

  1. Department of Pediatrics, Case Western Reserve University School of Medicine, Rainbow Babies and Childrens Hospital, 2101 Adelbert Road, 44106, Cleveland, Ohio, USA

    Thomas R. Shryock, Jeffrey D. Klinger & Mary Jane Thomassen

  2. Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Rainbow Babies and Childrens Hospital, 2101 Adelbert Road, 44106, Cleveland, Ohio, USA

    Jeffrey D. Klinger & Mary Jane Thomassen

  3. Department of Pediatrics, University of South Florida, College of Medicine, Tampa, Florida, USA

    James M. Sherman

Authors
  1. Thomas R. Shryock
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  2. James M. Sherman
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  3. Jeffrey D. Klinger
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  4. Mary Jane Thomassen
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Shryock, T.R., Sherman, J.M., Klinger, J.D. et al. Phagocytic inhibitory activity in serum of cats immunized withPseudomonas aeruginosa lipopolysaccharide. Current Microbiology 12, 91–95 (1985). https://doi.org/10.1007/BF01567398

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