Skip to main content
SpringerLink
Log in

Six complementation classes of conditionally lethal protein synthesis mutants of CHO cells selected by3H-amino acid

  • Published:
Somatic Cell Genetics

Abstract

Using a tritiated amino acid suicide procedure designed specifically to select conditional protein synthesis mutants, we have isolated and characterized a large number of such mutants of Chinese hamster ovary cells. All of the mutants are genetically stable and behave as recessives in somatic cell hybrids. Most of the new mutants are phenotypically dependent on the concentration of a specific amino acid as well as on temperature. In addition to identifying many additional leucyl- and asparagyl-tRNA synthetase mutants, complementation analysis has distinguished four new genetic classes representing methionine-, glutamine-, histidine-, and arginine-dependent mutants. Biochemical characterization of representative mutants from each of these six classes has identified the primary lesions as being defective aminoacyl-tRNA synthetases. Our selection results further demonstrate the high specificity of the3H-amino acid procedure for isolating protein synthesis mutants. Reconstruction experiments performed with two representative mutants indicated a selection efficiency of approximately 10% under standard conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature cited

  1. Basilico, C. (1977).Adv. Cancer Res. 24:222–266.

    Google Scholar 

  2. Thompson, L. H., and Baker, R. M. (1973). InMethods in Cell Biology, Vol. 6, (edited by D. M. Prescott) Academic Press, New York, pp. 209–281.

    Google Scholar 

  3. Thompson, L. H., Harkins, J. L., and Stanners, C. P. (1973).Proc. Natl. Acad. Sci. U.S.A. 70:3094–3098.

    PubMed  Google Scholar 

  4. Stanners, C. P., and Thompson, L. H. (1974). InControl of Proliferation in Animal Cells, Cold Spring Harbor Conferences on Cell Proliferation, Vol. 1, (edited by B. Clarkson and R. Baserga) Cold Spring Harbor Laboratory, pp. 191–203.

  5. Molnar, S. J., and Rauth, A. M. (1975).J. Cell Physiol. 85:173–178.

    PubMed  Google Scholar 

  6. Thompson, L. H., Stanners, C. P., and Siminovitch, L. (1975).Somat. Cell Genet. 1:187–208.

    PubMed  Google Scholar 

  7. McBurney, M. W., and Whitmore, G. F. (1976).Cell 2:173–182.

    Google Scholar 

  8. Taylor, R. T., and Hanna, M. L. (1977).Arch. Biochem. Biophys. 181:331–344.

    PubMed  Google Scholar 

  9. Thompson, L. H., Lofgren, D. J., and Adair, G. M. (1977).Cell 11:157–168.

    PubMed  Google Scholar 

  10. Stanners, C. P., Eliceiri, G. L., and Green, H. (1971).Nature (London) New Biol. 230:52–54.

    Google Scholar 

  11. Davidson, R. L., and Gerald, P. S. (1976).Somat. Cell Genet. 2:165–176.

    PubMed  Google Scholar 

  12. Davidson, R. L., O'Malley, K. A., and Wheeler, T. B. (1976).Somat. Cell Genet. 2:271–280.

    PubMed  Google Scholar 

  13. Kao, F. T., Chasin, L., and Puck, T. T. (1969).Proc. Natl. Acad. Sci. U.S.A. 64:1284–1291.

    PubMed  Google Scholar 

  14. Roufa, D. J., and Reed, S. J. (1975).Genetics 80:549–566.

    Google Scholar 

  15. Haralson, M. A., and Roufa, D. J. (1975).J. Biol. Chem. 250:8618–8623.

    PubMed  Google Scholar 

  16. Wasmuth, J. J., and Caskey, C. T. (1976).Cell 9:655–662.

    PubMed  Google Scholar 

  17. Gupta, R. S., and Siminovitch, L. (1976).Cell 9:213–221.

    PubMed  Google Scholar 

  18. Hankinson, O. (1976).Somat. Cell Genet. 2:497–507.

    Google Scholar 

  19. Patterson, D., Kao, F. T., and Puck, T. T. (1974).Proc. Natl. Acad. Sci. U.S.A. 71:2057–2061.

    PubMed  Google Scholar 

  20. Kao, F. T., and Puck, T. T. (1975).Genetics 79:343–352.

    PubMed  Google Scholar 

  21. Jones, C., and Moore, E. E. (1976).Somat. Cell Genet. 2:235–243.

    PubMed  Google Scholar 

  22. Patterson, D. (1976).Somat. Cell Genet. 2:41–53.

    PubMed  Google Scholar 

  23. Patterson, D. (1976).Somat. Cell Genet. 2:189–203.

    PubMed  Google Scholar 

  24. Patterson, D., and Jones, C. (1976).Somat. Cell Genet. 2:429–439.

    PubMed  Google Scholar 

  25. Patterson, D., Waldren, C., and Walker, C. (1976).Somat. Cell Genet. 2:113–123.

    PubMed  Google Scholar 

  26. Gupta, R. S., and Siminovitch, L. (1977).Cell 10:61–66.

    PubMed  Google Scholar 

  27. Jones, G. E., and Sargent, P. A. (1974).Cell 2:43–54.

    PubMed  Google Scholar 

  28. Stanley, P., Caillibot, V., and Siminovitch, L. (1975).Somat. Cell Genet. 1:3–26.

    PubMed  Google Scholar 

  29. Stanley, P., and Siminovitch, L. (1977).Somat. Cell Genet. 3:391–406.

    PubMed  Google Scholar 

  30. Flintoff, W. F., Spindler, S. M., and Siminovitch, L. (1976).In Vitro 12:749–757.

    PubMed  Google Scholar 

  31. Siminovitch, L. (1976).Cell 7:1–11.

    PubMed  Google Scholar 

  32. Worten, R. G., Ho, C. C., and Duff, C. (1977).Somat. Cell Genet. 3:27–45.

    PubMed  Google Scholar 

  33. Chasin, L. A. (1973).J. Cell Physiol. 82:299–308.

    PubMed  Google Scholar 

  34. Hsie, A. W., Brimer, P. A., Mitchell, T. J., and Gosslee, D. G. (1975).Somat. Cell Genet. 1:247–261.

    PubMed  Google Scholar 

  35. Denney, R. M., and Craig, I. W. (1976).Biochem. Genet. 14:99–117.

    PubMed  Google Scholar 

  36. Shimizu, N., Kucherlapati, R. S., and Ruddle, F. H. (1976).Somat. Cell Genet. 2:345–357.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biomedical Sciences Division, L-452, University of California, Lawrence Livermore Laboratory, 94550, Livermore, California

    Gerald M. Adair, Larry H. Thompson & Patricia A. Lindl

Authors
  1. Gerald M. Adair
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Larry H. Thompson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Patricia A. Lindl
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Adair, G.M., Thompson, L.H. & Lindl, P.A. Six complementation classes of conditionally lethal protein synthesis mutants of CHO cells selected by3H-amino acid. Somat Cell Mol Genet 4, 27–44 (1978). https://doi.org/10.1007/BF01546491

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01546491

Keywords

Search

Navigation