Abstract
Forty-three temperature-sensitive mutants were isolated in the CHO cell line by selecting for noncycling cells using [3H]TdR and cytosine arabinoside. Cell division was extremely temperature sensitive in eight of the mutants, and these were studied in more detail. In seven of these eight mutants, the in vitro specific activity of a single aminoacyl-tRNA synthetase was greatly reduced; four had reduced levels of histidyl-tRNA synthetase, two of valyl-tRNA synthetase, and one of leucyl-tRNA synthetase. Cell hybridization studies showed that the mutants formed three complementation groups. In six of the seven mutants the aminoacyl-tRNA synthetase which had reduced activity was also more thermolabile than the wild-type enzyme. The spontaneous reversion frequency was low for these mutants, and in most cases could be increased by treatment with a chemical mutagen. The isolation of the valyl-tRNA synthetase mutant reported here brings to eight the number of different aminoacyl-tRNA synthetase mutants isolated in the CHO cell line.
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Literature cited
Thompson, L. H., and Baker, R. M. (1973). InMethods in Cell Biology, Vol. 6, (ed.) Prescott, D. M. (Academic Press, New York) pp. 209–281.
Basilico, C. (1977).Adv. Cancer Res. 24:223.
Wasmuth, J. J., and Caskey, C. T. (1976).Cell 9:655.
Haralson, M. A., and Roufa, D. J. (1975).J. Biol. Chem. 250:8618.
Liskay, R. M. (1974).J. Cell Physiol. 84:49.
Hatzfeld, J., and Buttin, G. (1975).Cell 5:123.
Thompson, L. H., Stanners, C. P. and Simonvitch, L. (1975).Somat. Cell Genet. 1:187.
Thompson, L. H., Lofgren, D. J., and Adair, G. M. (1977).Cell 11:157.
Hankinson, O. (1976).Somat. Cell Genet. 2:497.
Sato, K. (1975).Nature 257:813.
Kao, F. T., and Puck, T. T. (1968).Proc. Natl. Acad. Sci. U.S.A. 60:1275.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951).J. Biol. Chem. 193:265.
Davidson, R. L., and Gerald, P. S. (1976).Somat. Cell Genet. 2:165.
Baker, R. M., Brunette, D. M., Mankovitz, R., Thompson, L. H., Whitmore, G. F., Simonovitch, L., and Till, J. E. (1974).Cell 1:9.
Fincham, J. R. S. (1966).Genetic Complementation. (W. A. Benjamin, New York).
Söll, D., and Schimmel, P. R. (1974). InThe Enzymes, Vol. 10, (ed). Boyer, P. D. (Academic Press, New York) pp. 489–538.
Kaplan, S., and Anderson, D. (1968).J. Bacteriol. 95:991.
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Ashman, C.R. Mutations in the structural genes of CHO cell histidyl-, valyl-, and leucyl-tRNA synthetases. Somat Cell Mol Genet 4, 299–311 (1978). https://doi.org/10.1007/BF01542844
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DOI: https://doi.org/10.1007/BF01542844