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Interleukin-1β induces expression of cyclooxygenase-2 mRNA in human gingival fibroblasts

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Abstract

The effect of interleukin-1β (IL-1β) on the expression of cyclooxygenase-1 and −2 (COX-1 and COX-2) mRNA and its relation to prostaglandin E2 (PGE2) biosynthesis in human gingival fibroblasts was studied. IL-1β increased levels of mRNA for COX-2 whereas the COX-1 mRNA level was unaffected. The increased COX-2 mRNA levels were accompanied by enhanced PGE2 formation. The phorbol, 12-myristate 13-acetate (PMA), known to stimulate protein kinase C (PKC), also induced expression of COX-2 mRNA. When gingival fibroblasts were treated simultaneously with IL-1β and PMA, the cytokine IL-1β synergistically increased levels of COX-2 mRNA, accompanied by a corresponding increase in PGE2 biosynthesis. The anti-inflammatory steroid, dexamethasone (DEX) abolished the enhanced expression of COX-2 mRNA as well as PGE2 formation induced by IL-1β, PMA or the combination of IL-1β and PMA. The study indicates that the IL-1β induced PGE2 formation is mediated by an enhanced gene expression of COX-2 in gingival fibroblasts suggesting that the enzyme COX-2 may play an important role in the regulation of prostanoid formation at inflammatory lesions in gingival tissue.

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Authors and Affiliations

  1. Department of Orthodontics and Pediatric Dentistry Faculty of Odontology, Karolinska Institute, Sweden

    Tülay Yucel-Lindberg & Thomas Modéer

  2. Karo Bio, Novum

    Harri Ahola & Stefan Nilsson

  3. Department of Medical Nutrition, Karolinska Institute, Huddinge, Sweden

    Jan Carlstedt-Duke

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  1. Tülay Yucel-Lindberg
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  2. Harri Ahola
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  3. Stefan Nilsson
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  4. Jan Carlstedt-Duke
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  5. Thomas Modéer
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Yucel-Lindberg, T., Ahola, H., Nilsson, S. et al. Interleukin-1β induces expression of cyclooxygenase-2 mRNA in human gingival fibroblasts. Inflammation 19, 549–560 (1995). https://doi.org/10.1007/BF01539135

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