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Role of cholecystokinin in bombesin- and meal-stimulated pancreatic polypeptide secretion in dogs

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Abstract

This study was undertaken to delineate the role of cholecystokinin (CCK) in bombesin- and meal-stimulated pancreatic polypeptide (PP) secretion in seven conscious dogs by studying: (1) the stimulatory effect of similar plasma levels of CCK induced by a meal and infusions of bombesin and the synthetic CCK analog cerulein on plasma PP, and (2) the inhibition of PP secretion by the CCK-receptor antagonist CR-1409 during these three stimuli. The stimulation of PP secretion during bombesin (11.0±1.6 nM/hr) and after the meal (8.9±2.0 nM/hr) were significantly (P<0.05) greater than during infusion of the CCK analog cerulein (2.7±0.4 nM/hr). CR-1409 significantly inhibited the bombesin- and meal-stimulated PP secretion to 2.0±0.4 nM/hr (81%; P<0.05) and 3.1±1.2 nM/hr (47%; P<0.05), respectively, while the cerulein-stimulated PP release was almost abolished to 0.2±0.1 nM/hr (93%; P<0.05) by the drug. These findings point to an important role for CCK in the regulation of bombesin- and meal-stimulated PP secretion.

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References

  1. Lin TM, Evans DC, Chance RE, Spray GF: Bovine pancreatic peptide: Action on gastric and pancreatic secretion in dogs. Am J Physiol 232:E311–315, 1977

    PubMed  Google Scholar 

  2. Larsson LI, Sundler F, Hakanson R: Pancreatic polypeptide: A postulated new hormone: Identification of its cellular storage site by light and electron microscopic immunocytochemistry. Diabetologia 12:211–226, 1976

    PubMed  Google Scholar 

  3. Greenberg GR, McCloy RF, Adrian TE, Chadwick VS, Baron JH, Bloom SR: Inhibition of pancreas and gallbladder by pancreatic polypeptide. Lancet 2:1280–1282, 1978

    PubMed  Google Scholar 

  4. De Jong AJL, Singer MV, Lamers CBHW: Effect of rabbit anti-pancreatic polypeptide serum on postprandial pancreatic exocrine secretion in dogs. Gastroenterology 90:1926–1931, 1986

    PubMed  Google Scholar 

  5. Yanaihara H, Yanaihara C, Mochizuki T, Iwahara K, Fujita T, Iwanaga T: Immunoreactive GRP. Peptides 2:185–191, 1981

    PubMed  Google Scholar 

  6. Floyd JC, Fajans SS, Pek S, Chance RE: A newly recognized pancreatic polypeptide; plasma levels in health and disease. Recent Prog Horm Res 33:519–570, 1977

    Google Scholar 

  7. De Jong AJL, Singer MV, Lamers CBHW: Effect of pancreatic polypeptide-antiserum on bombesin-stimulated pancreatic exocrine secretion in dogs. Peptides 8:973–976, 1987

    PubMed  Google Scholar 

  8. Schwartz TW, Holst JJ, Fahrenkrug J, Lindkaer Jensen S, Nielson OV, Rehfeld JF, Stadil F: Vagal, cholinergic regulation of pancreatic polypeptide secretion. J Clin Invest 61:769–781, 1978

    Google Scholar 

  9. Adrian TE, Bloom SR, Hermanson K, Iversen J: Pancreatic polypeptide, glucagon and insulin secretion from the isolated perfused canine pancreas. Diabetologia 14:413–417, 1978

    PubMed  Google Scholar 

  10. Lonovics J, Guzman S, Devitt P, Hejtmancik KE, Suddith RL, Rayford PL, Thompson JC: Release of pancreatic polypeptide in humans by infusion of cholecystokinin. Gastroenterology 79:817–822, 1980

    PubMed  Google Scholar 

  11. Makovec F, Bani M, Cereda R, Chiste R, Pacini MA, Revel L, Rovati LA, Rovati LC, Setnikar I: Pharmacological properties of lorglumide as a member of a new class of cholecystokinin antagonists. Drug Res 37:1265–1268, 1987

    Google Scholar 

  12. Beglinger C, Taylor IL, Grossman MI, Solomon TE: Pancreatic polypeptide inhibits exocrine pancreatic responses to six stimulants. Am J Physiol 246:G286-G291, 1984

    PubMed  Google Scholar 

  13. Solomon TE, Yamada T, Elashoff J, Wood J, Beglinger C: Bioactivity of cholecystokinin analogues: CCK-8 is not more potent than CCK-33. Am J Physiol 247:G105-G111, 1984

    PubMed  Google Scholar 

  14. Hildebrand P, Beglinger C, Köhler E, Setnikar I, Gyr K: Biological effects of a proglumide derivate as cholecystokinin antagonist in conscious dogs. Regul Pept 18:213–220, 1987

    PubMed  Google Scholar 

  15. Jansen JBMJ, Lamers CBHW: Radioimmunoassay of cholecystokinin in human tissue and plasma. Clin Chim Acta 131:305–316, 1983

    PubMed  Google Scholar 

  16. Lamers CBHW, Diemel JM, van Leer E, van Leusen R, Peetoom J: Mechanism of elevated serum pancreatic polypeptide concentrations in chronic renal failure. J Clin Endocrinol Metab 55:922–926, 1982

    PubMed  Google Scholar 

  17. Beglinger C, Fried M, Whitehouse J, Jansen JB, Lamers CB, Gyr K: Pancreatic enzyme response to a liquid meal and to hormonal stimulation; correlation with plasma secretin and cholecystokinin levels. J Clin Invest 75:1471–1476, 1985

    PubMed  Google Scholar 

  18. Konturek SJ, Tasler J, Cieszkowski M, Szewczyk K, Hlady M: Effect of cholecystokinin receptor antagonist on pancreatic responses to exogenous gastrin and cholecystokinin and to meal stimuli. Gastroenterology 94:1014–1023, 1988

    PubMed  Google Scholar 

  19. Hosotani R, Chowdhury P, Rayford PL: L-364,718, a new CCK antagonist, inhibits postprandial pancreatic secretion and PP release in dogs. Dig Dis Sci 34:462–467, 1989

    PubMed  Google Scholar 

  20. Konturek SJ, Konturek P, Bielanski W, Szewczyk K: CCK receptors in release of pancreatic polypeptide (PP) in dogs. Dig Dis Sci 34:849–856, 1989

    PubMed  Google Scholar 

  21. Konturek SJ, Tasler J, Konturek JW, Cieszkowski M, Szewczyk K, Hladij M, Anderson PS: Effect of non-peptidal CCK receptor antagonist (L-364, 718) on pancreatic responses to cholecystokinin, gastrin, bombesin, and meat feeding in dogs. Gut 30:110–117, 1989

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Gastroenterology and Hepatology, University Hospital, Building 1, C4-P015, P.O. Box 9600, 2300, RC Leiden, The Netherlands

    Jan B. M. J. Jansen MD, Alphons J. L. DE Jong MD, Manfred V. Singer MD, Wolfgang Niebel MD, Lucio C. Rovati MD &  Cornelis B. H. W. Lamers MD

  2. Division of Gastroenterology, Department of Medicine, University of Essen, FRG

    Jan B. M. J. Jansen MD, Alphons J. L. DE Jong MD, Manfred V. Singer MD, Wolfgang Niebel MD, Lucio C. Rovati MD &  Cornelis B. H. W. Lamers MD

  3. Rotta Research Laboratories, Monza, Italy

    Jan B. M. J. Jansen MD, Alphons J. L. DE Jong MD, Manfred V. Singer MD, Wolfgang Niebel MD, Lucio C. Rovati MD &  Cornelis B. H. W. Lamers MD

Authors
  1. Jan B. M. J. Jansen MD
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  2. Alphons J. L. DE Jong MD
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  3. Manfred V. Singer MD
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  4. Wolfgang Niebel MD
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  5. Lucio C. Rovati MD
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  6. Cornelis B. H. W. Lamers MD
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Additional information

Supported by grant 13-37-43 from the Netherlands Foundation for Medical Research MEDIGON and grant Si 228/7-1 from the Deutsche Forschungsgemeinschaft.

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Jansen, J.B.M.J., DE Jong, A.J.L., Singer, M.V. et al. Role of cholecystokinin in bombesin- and meal-stimulated pancreatic polypeptide secretion in dogs. Digest Dis Sci 35, 1073–1077 (1990). https://doi.org/10.1007/BF01537577

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  • DOI: https://doi.org/10.1007/BF01537577

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