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Increase in clonal variation in Chinese hamster ovary cells after treatment with mutagens

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Somatic Cell and Molecular Genetics

Abstract

Clonal variation has been studied in CHO cells. The variant phenotype was an altered morphology of clones in agar: the parental CHO cells give rise to solid clumps of cells (wild-type colonies); occasionally, dispersed colonies arise, and the cells display an invasive growth in agar (INGA-type colonies). The frequency of this altered phenotype can be enhanced by treatment with a variety of mutagens (EMS, ENU, 4NQO, N-Ac-AAF, ultraviolet light, and X-irradiation). Enhancement was not due to a selective killing of wild-type cells or to a side-effect of cytotoxicity, which suggests that DNA damage is the cause of the altered phenotype. The INGA-trait breeds true, but most of the isolated clones have an inherent instability.

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Authors and Affiliations

  1. Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, P.O. Box 9503, 2300, RA Leiden, The Netherlands

    Małgorzata Z. Zdzienicka, M. Cupido & J. W. I. M. Simons

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  1. Małgorzata Z. Zdzienicka
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  2. M. Cupido
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  3. J. W. I. M. Simons
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Zdzienicka, M.Z., Cupido, M. & Simons, J.W.I.M. Increase in clonal variation in Chinese hamster ovary cells after treatment with mutagens. Somat Cell Mol Genet 11, 127–134 (1985). https://doi.org/10.1007/BF01534701

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  • DOI: https://doi.org/10.1007/BF01534701

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