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Assignment of gene coding human T-cell differentiation antigen, Tpl20, to chromosome 11

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Somatic Cell and Molecular Genetics

Abstract

A pan T-cell antigen with a molecular weight of 120 kilodaltons (kd) is recognized by a monoclonal antibody, Tp120, produced in our laboratories. Two hybrid clones reactive with this Tp120 antibody were established from the fusion between concanavalin A-stimulated human peripheral blood lymphocytes and hypoxanthine-guanine phosphoribosyltransferase-deficient mouse T cell leukemia, BW5147. These two clones were also positive with two other antibodies, 12.1 and T12, both of which detect 120kd pan T-cell antigen. Karyotype analysis showed that one clone retained human chromosomes 6, 7pq−, and 11, and the other maintained chromosomes 11 and 21. As soon as both of these clones lost the chromosome 11, the expression of Tp120 became negative. The presence of human chromosome 11 was confirmed by the isozyme analysis of lactate dehydrogenase-A. The results indicated that the presence of chromosome 11 was essential for expression of 120kd pan T-cell antigen.

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Authors and Affiliations

  1. Department of Pediatrics, Nagoya University School of Medicine, 466, Nagoya

    Ikuya Tsuge

  2. Laboratories of Cell Biology, Aichi Cancer Center Research Institute, 464, Nagoya, Japan

    Kazuhiko R. Utsumi & Toshitada Takahashi

  3. Laboratories of Chemotherapy, Aichi Cancer Center Research Institute, 464, Nagoya, Japan

    Ryuzo Ueda & Shigeru Takamoto

Authors
  1. Ikuya Tsuge
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  2. Kazuhiko R. Utsumi
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  3. Ryuzo Ueda
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  4. Shigeru Takamoto
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  5. Toshitada Takahashi
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Tsuge, I., Utsumi, K.R., Ueda, R. et al. Assignment of gene coding human T-cell differentiation antigen, Tpl20, to chromosome 11. Somat Cell Mol Genet 11, 217–222 (1985). https://doi.org/10.1007/BF01534678

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  • DOI: https://doi.org/10.1007/BF01534678

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