Summary
Two virus strains which had been suspected to be the etiological agents of infectious bursitis (Gumboro disease) and of inclusion body hepatitis of chickens were characterized by their morphology, their peptide composition and the segmented genome of their double-stranded RNA to be typical reoviruses. Although the 2 avian strains did not clearly differ in their serological behaviour, the size of some of their RNA segments were not identical. Both strains replicated in tissue cultures prepared from the chorioallantoic membrane of embryonated eggs with growth characteristics of reoviruses.
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References
Almeida, J. D., Morris, R.: Antigenically-related viruses associated with infectious bursal disease. J. gen. Virol.20, 369–375 (1973).
Bellamy, A. R., Shapiro, L., August, J. T., Joklik, W. K.: Studies on reovirus RNA. Characterization of reovirus genome RNA. J. mol. Biol.29, 1–17 (1967).
Bellamy, A. R., Hole, L. V., Baguley, B. C.: Isolation of the trinucleotide pppGpCpU from reovirus. Virology42, 415–420 (1970).
Cursiefen, D., Becht, H.:In vitro cultivation of cells from the chorioallantoic membrane of chick embryos. Med. Microbiol. Immunol.161, 3–10 (1975).
Kawamura, H., Shimizu, F., Maeda, M., Tsubahara, H.: Avian reovirus: Its properties and serological classification. Nat. Inst. Anim. Hlth. Quart.5, 115–124 (1965).
Kösters, J., Becht, H., Rudolph, R.: Properties of the infectious bursal agent of chicken (IBA). Med. Microbiol. Immunol.157, 291–298 (1972).
Loening, U. E.: The fractionation of high-molecular weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem. J.102, 251–257 (1967).
Martin, S. A., Zweerink, H. J.: Isolation and characterization of two types of bluetongue virus particles. Virology50, 495–506 (1972).
Peacock, A. C., Dingman, C. W.: Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis. Biochemistry6, 1818–1827 (1967).
Scholtissek, C., Rott, R.: Hybridization studies with influenza virus RNA. Virology39, 400–407 (1969).
Sekiguchi, K., Koide, F., Kawamura, H.: Physico-chemical properties of avian reovirus and its nucleic acid. Arch. ges. Virusforsch.24, 123–136 (1968).
Shatkin, A. J.: Inactivity of purified reovirus RNA as a template for E. coli polymerasesin vitro. Proc. Nat. Acad. Sci. U.S.54, 1721–1728 (1965).
Shatkin, A. J., Sipe, J. D., Loh, P.: Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis. J. Virol.2, 986–991 (1968).
Smith, R. E., Zweerink, H. J., Joklik, W. K.: Polypeptide components of virions, top component, and cores of reovirus type 3. Virology39, 791–810 (1969).
Woernle, H., Brunner, A., Kussmaul, K.-F.: Nachweis aviärer Reo-Viren im Agar-Gel-Präzipitationstest. Tierärztl. Umsch.29, 307–312 (1974).
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Nick, H., Cursiefen, D. & Becht, H. Structural and growth characteristics of two avian reoviruses. Archives of Virology 48, 261–269 (1975). https://doi.org/10.1007/BF01317969
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DOI: https://doi.org/10.1007/BF01317969