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Inhibitors of foot-and-mouth disease virus

II. Temperature-dependence of the effect of guanidine on virus growth

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Summary

In suspended secondary calf kidney cells infected with foot-and-mouth disease virus (FMDV) the temperature range for optimal virus growth is shifted down by 3 to 5° C in the presence of 1–2 mM guanidine. For some virus strains this shift is so effective that at infraoptimal temperatures virus yield in guanidine-treated cells exceeds that of the corresponding control by more than one log10. On the contrary, at supraoptimal temperatures inhibition of virus growth by the drug is strongly enhanced.

At a concentration of 1 to 2 mM guanidine virus yield reduction or enhancement is based on a decrease or increase, respectively, of the number of virus producing cells (infective centers; I.C.), while virus yield per I.C. is less affected.

Besides this ‘thermomimetic’ effect virus production is inhibited by guanidine depending on the concentration of this substance.

A mutant of FMDV strain O1L, resistant to 4.2 mM guanidine, did not differ from the original virus in its antigenic behaviour in the passive immunohemolysis test.

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References

  1. Adler R., Garfinkle, B. D., Mitchell, W., Tershak, D. R.: Degradation of poliovirus RNAin vivo. Canad. J. Microbiol.19, 539–540 (1973).

    Google Scholar 

  2. Ahl, R.: Inhibitors of foot-and-mouth disease virus. I. Effect of EDTA, temperature, and interferon on the viral growth cycle. Arch. ges. Virusforsch.46, 302–314 (1974).

    Google Scholar 

  3. Baltimore, D.: The replication of picornaviruses. In:Levy, H. B. (ed.), The biochemistry of viruses. New York-London: Marcel Dekker 1969.

    Google Scholar 

  4. Caliguiri, L. A., Tamm, I.: Action of guanidine on the replication of poliovirus RNA. Virology35, 408–417 (1968).

    Google Scholar 

  5. Caliguiri, L. A., Tamm, I.: Distribution and translation of poliovirus RNA in guanidine-treated cells. Virology36, 223–231 (1968).

    Google Scholar 

  6. Cooper, P. D., Steiner-Pryor, A., Wright, P. J.: A proposed regulator for poliovirus: The equestron. Intervirology1, 1–10 (1973).

    Google Scholar 

  7. Delagneau, J.-F.: Effects of temperature on viral RNA synthesis and RNA polymerase activity in a foot-and-mouth disease virus system. Arch. ges. Virusforschung32, 318–328 (1970).

    Google Scholar 

  8. Dinter, Z., Bengtsson, S.: Suppression of the inhibitory action of guanidine on virus multiplication by some amino acids. Virology24, 254–261 (1964).

    Google Scholar 

  9. Dulbecco, R., Vogt, M.: Plaque formation and isolation of pure lines with poliomyelitis viruses. J. exp. Med.99, 167–182 (1954).

    Google Scholar 

  10. Fiszman, M. Y., Bucchini, D., Girard, M., Lwoff, A.: Inhibition of poliovirus RNA synthesis by supraoptimal temperatures. J. gen. Virol.6, 293–304 (1970).

    Google Scholar 

  11. Fiszman, M., Reynier, M., Bucchini, D., Girard, M.: Thermosensitive block of the Sabin strain of poliovirus type I. J. Virol.10, 1143–1151 (1972).

    Google Scholar 

  12. Garfinkle, B. D., Tershak, D. R.: Effect of temperature on the cleavage of polypeptides during growth of LSc poliovirus. J. molec. Biol.59, 537–541 (1971).

    Google Scholar 

  13. Garfinkle, B. D., Tershak, D. R.: Degradation of poliovirus polypeptidesin vivo. Nature (New Biol.)238, 206–208 (1972).

    Google Scholar 

  14. Gevaudan, P., Charrel, J., Pieroni, G.: Recherche sur un variant “froid” de virus coxsackie. Ann. Inst. Pasteur114, 209–215 (1968).

    Google Scholar 

  15. Gevaudan, P., Pieroni, G., Charrel, J., Gevaudan, M. J.: Effect des températures infra- et supraoptimales sur le développement du coxsackievirus. Ann. Inst. Pasteur117, 507–511 (1969).

    Google Scholar 

  16. Ghendon, Y. Z., Ageeva, O.: Synthesis of cellular and viral macromolecules under conditions of suboptimal temperature. Arch. ges. Virusforsch.30, 7–15 (1970).

    Google Scholar 

  17. Groman, N., Lwoff, A., Lwoff, M.: Recherches sur un variant dit “froid” du virus de la poliomyélite. Ann. Inst. Pasteur98, 351–359 (1960).

    Google Scholar 

  18. Koschel, K., Wecker, E.: Early functions of poliovirus. III. The effect of guanidine on early functions. Z. Naturforsch.26B, 940–944 (1971).

    Google Scholar 

  19. Lab, M., Kirn, A.: Variations de la thermosensibilité du développement du virus Sindbis en fonction du système cellulaire. Comp. rend. Acad. Sci. (Paris)268, 2624 to 2627 (1869).

    Google Scholar 

  20. Loddo, B., Gessa, G. L., Schivo, M. L., Spanedda, A., Brotzu, G., Ferrari, W.: Antagonism of the guanidine interference with poliovirus replication by simple methylated and ethylated compounds. Virology28, 707–712 (1966).

    Google Scholar 

  21. Lwoff, A.: The thermosensitive critical event of the viral cycle. Cold Spring Harbor Symp. on Quant. Biol.27, 159–172 (1962).

    Google Scholar 

  22. Lwoff, A.: The specific effectors of viral development. Biochem. J.96, 289–301 (1965).

    Google Scholar 

  23. Lwoff, A.: Death and transfiguration of a problem. Bact. Rev.33, 390–403 (1969).

    Google Scholar 

  24. Lwoff, A., Lwoff, M.: Les événements cycliques du cycle viral. II. Les effets de l'eau lourde. Ann. Inst. Pasteur101, 478–489 (1961).

    Google Scholar 

  25. Lwoff, A., Lwoff, M.: Inhibition de la multiplication du poliovirus par l'urée considérée comme substance thermomimetique. Comp. Rend. Acad. Sci. (Paris)254, 771–773 (1962).

    Google Scholar 

  26. Lwoff, A., Lwoff, M.: Neutralisation par divers métabolites de l'effet inhibiteur de la guanidine sur le développement du poliovirus. Comp. Rend. Acad. Sci. (Paris)259, 949–952 (1964).

    Google Scholar 

  27. Marcialis, M. A., Schivo, M. L., Cioglia, A. M., Atzeni, A., Loddo, B.: Irreversible impairment produced by guanidine on the functions of poliovirus proteins. Experientia29, 1561–1563 (1973).

    Google Scholar 

  28. Mikheyeva, A., Ghendon, Y.: The influence of supraoptimal temperature on poliovirus type 1 Mahoney strain reproduction. Arch. ges. Virusforsch.45, 65–77 (1974).

    Google Scholar 

  29. Moser, A. G., Caliguiri, L. A., Tamm, I.: Blocking of guanidine action on poliovirus multiplication. Virology45, 653–663 (1971).

    Google Scholar 

  30. Philipson, L., Bengtsson, S., Dinter, Z.: The reversion of guanidine inhibition of poliovirus synthesis. Virology29, 317–329 (1966).

    Google Scholar 

  31. Polatnick, J., Arlinghaus, R. B.: Effect of foot-and-mouth disease virus on protein synthesis and ribonucleic acid polymerase activity at various temperatures. Proc. Soc. exp. Biol. (N.Y.)134, 5–8 (1970).

    Google Scholar 

  32. Reda, I. M., Wittmann, G.: Die Verwendung von CrCl3 zur Kopplung von Maulund Klauenseuche-Virus an Erythrozyten und deren Eignung für die passive Hämagglutination and Immunhämolyse. Arch. ges. Virusforsch.38, 205–215 (1972).

    Google Scholar 

  33. Röder, A., Koschel, K.: Virus-specific proteins associated with the replication complex of poliovirus RNA. J. gen. Virol.28, 85–98 (1975).

    Google Scholar 

  34. Stoker, M., McPherson, I.: Syrian hamster fibroblast cell line BHK-21 and its derivatives. Nature203, 1355–1357 (1964).

    Google Scholar 

  35. Tershak, D. R.: Synthesis of ribonucleic acid in cells infected with LSc poliovirus at elevated temperatures. J. Virol.3, 297–303 (1969).

    Google Scholar 

  36. Vande Woude, G. F., Ascione, R.: Translation products of foot-and-mouth disease virus-infected baby hamster kidney cells. Arch. ges. Virusforsch.45, 259–271 (1974).

    Google Scholar 

  37. Wagner, G. G., Card, J. L., Cowan, K. M.: Immunological studies of foot-and-mouth disease. VII. Characterization of foot-and-mouth disease virus concentrated by polyethyleneglycol precipitation. Arch. ges. Virusforsch.30, 343–352 (1970).

    Google Scholar 

  38. Wittmann, G.: Genetische Merkmale attenuierter Maul- und Klauenseuche-(MKS)-Viren. Die Virusvermehrung bei 40°, 37°, 28° und 22° C in Gewebekulturen. Zbl. Bakt. I. Abt. Orig.196, 400–410 (1965).

    Google Scholar 

  39. Yakobson, E. A., Mikheyeva, A. V., Ghendon, Y. Z.: Some properties of temperature-sensitive mutants of poliovirus in two cell lines. Acta virol.16, 345 to 348 (1972).

    Google Scholar 

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  1. Bundesforschungsanstalt für Viruskrankheiten der Tiere, Tübingen, Germany

    H. Nick & R. Ahl

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  1. H. Nick
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Nick, H., Ahl, R. Inhibitors of foot-and-mouth disease virus. Archives of Virology 52, 71–83 (1976). https://doi.org/10.1007/BF01317866

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