Summary
Four epidemic seasons of rotaviruses were studied in Helsinki during 1986–1990. This is the first Scandinavian study, where both electropherotypes and serotypes are determined. Out of 5316 fecal specimens 769 (14.5%) rotavirus positive samples were detected by electron microscopy. Of these, 645 isolates (83.9%) gave a clear RNA pattern in gel electrophoresis and they clustered into 87 electropherotypes. An illustrative number of isolates representing each electropherotype (=E-type) was serotyped using VP7 protein-specific monoclonal antibodies for serotypes G1–G4 and without exceptions, within one E-type only a single serotype specificity was found. After establishment of the serotype of each E-type, the distribution of serotypes was scored as 61.2%, 2.0%, 0.5% and 29.8% for G1–G4, respectively; 6.5% remained untypable. Two seasons had one predominant E-type (Season 1, 1986–87, and Season 3, 1988–89, 84.2% and 80.6% of rotavirus positive samples, respectively). Both were followed by a season with no predominant E-type, but several minor E-types. Altogether, 5 short E-types (13/645 samples) with serotype G2 specificity were found, most of them occurring in Season 2. Only 2 E-types (3 samples) belonged to serotype G3. Group C rotavirus was found in 8 specimens. In this study a shift in serotypes, from G1 to G4, was observed in Finland in 1988/89; a similar shift was reported in many European countries at that time.
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References
Arista S, Nicolas JC, Giovannelli L, Chiarini A (1983) Electropherotypes of human rotaviruses isolated in Palermo, Italy. Ann Virol Pasteur 134E: 549–556
Beards G, Xu L, Ballard A, Desselberger U, McCrae MA (1992) A serotype 10 human rotavirus. J Clin Microbiol 30: 1432–1435
Beards GM, (1982) Polymorphism of genomic RNAs within rotavirus serotypes and subgroups. Arch Virol 74: 65–70
Beards GM, Desselberger U, Flewett TH (1989) Temporal and geographical distributions of human rotavirus serotypes, 1983 to 1988. J Clin Microbiol 27: 2827–2833
Begue R, Dennehy PH, Huang J, Martin P (1992) Serotype variation of group A rotaviruses over nine winter epidemics in Southeastern New England. J Clin Microbiol 30: 1592–1594
Bingnan F, Unicomb L, Rahim Z, Banu NN, Podder G, Clemens J, van Loon FPL, Rao MR, Malek A, Tzipori S (1991) Rotavirus-associated diarrhoea in rural Bangladesh: two-year study of incidence and serotype distribution. J Clin Microbiol 29: 1359–1363
Bishop RF, Unicomb LE, Barnes GL (1991) Epidemiology of rotavirus serotypes in Melbourne, Australia, from 1973 to 1989. J Clin Microbiol 29: 862–868
Coulson B (1987) Variation in neutralization epitopes of human rotaviruses in relation to genomic RNA polymorphism. Virology 159: 209–216
Dunn SJ, Ward RL, McNeal MM, Cross TL, Greenberg HB (1993) Identification of a new neutralization epitope on VP7 of human serotype 2 rotavirus and evidence for electropherotype differences caused by single nucleotide substitutions. Virology 197: 397–404
Flores J, Greenberg HB, Myslinski J, Kalica AR, Wyatt RG, Kapikian AZ, Chanock RM (1982) Use of transcription probes for genotyping rotavirus reassortants. Virology 121: 288–295
Gerna G, Arista S, Passarani N, Sarasini A, Battaglia M (1987) Electropherotype heterogeneity within serotypes of human rotavirus strains circulating in Italy. Arch Virol 95: 129–135
Gerna G, Forster J, Parea M, Sarasini A, di Matteo A, Baldanti F, Langosch B, Schmidt S, Battaglia M (1990) Nosocomial outbreak of neonatal gastroenteritis caused by a new serotype 4, subtype 4B human rotavirus. J Med Virol 31: 175–182
Gerna G, Sarasini A, Arista S, di Matteo A, Giovannelli L, Parea M, Halonen P (1990) Prevalence of human rotavirus serotypes in some European countries 1981–1988. Scand J Infect Dis 22: 5–10
Gerna G, Sarasini A, di Matteo A, Parea M, Orsolini P, Battaglia M (1988) Identification of two subtypes of serotype 4 human rotavirus by using VP7-specific neutralizing monoclonal antibodies. J Clin Microbiol 26: 1388–1392
Gerna G, Sarasini A, Parea M, Arista S, Miranda P, Brussow H, Hoshino Y, Flores J (1992) Isolation and characterization of two distinct human rotavirus strains with G6 specificity. J Clin Microbiol. 30: 9–16
Gerna G, Sarasini A, Zentilin L, di Matteo A, Miranda P, Parea M, Battaglia M, Milanesi G (1990) Isolation in Europe of 69 M-like (serotype 8) human rotavirus strains with either subgroup I or II specificity and a long RNA electropherotype. Arch Virol 112; 27–40
Gorziglia M, Larralde G, Kapikian AZ, Chanock RM (1990) Antigenic relationships among human rotaviruses as determined by outer capsid protein VP4. Proc Natl Acad Sci USA 87: 7155–7159
Gouvea V, Ho MS, Glass R, Woods P, Forrester B, Robinson C, Ashley R, Riepenhoff-Talty M, Clark HF, Taniguchi K, Meddix E, McKellar B, Pickering L (1990) Serotypes and electropherotypes of human rotavirus in the USA: 1987–1989. J Infect Dis 162: 362–367
Graham A, Kudesia G, Allen AM, Desselberger U (1987) Reassortment of human rotavirus possessing genome rearrangements with bovine rotavirus: evidence for host cell selection. J Gen Virol 68: 115–122
Green KY, Sarasini A, Qian Y, Gerna G (1992) Genetic variation in rotavirus serotype 4 subtypes. Virology 188: 362–368
Greenberg HB, McAuliffe V, Valdesuso J, Wyatt R, Flores J, Kalica A, Hoshino Y, Singh N (1983) Serological analysis of the subgroup proteins of rotavirus, using monoclonal antibodies. Infect Immun 39: 91–99
Herring AJ, Inglis NF, Ojeh CK, Snodgrass DR, Menzies JD (1982) Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J Clin Microbiol 16: 473–477
Hung T, Changarn W, Zhaoying F, Zinyi C, Xuejian C, Tungxin C, Weiwe Y, Shuaseu D, Weicheng C (1984) Waterborne outbreak of rotavirus diarrhoea in adults in China caused by a novel rotavirus. Lancet i: 1139–1142
Kalica AR, Greenberg HB, Espejo RT, Flores J, Wyatt RG, Kapikian AZ, Chanock RM (1981) Distinctive ribonucleic acid patterns of human rotavirus subgroups 1 and 2. Infect Immun 33: 958–961
Kalica AR, Wyatt RG, Kapikian AZ (1978) Detection of differences among human and animal rotaviruses, using analysis of viral RNA. J Am Vet Med Ass 173: 531–537
Kapikian AZ (1994) Viral infections of the gastrointestinal tract, 2nd ed. Marcel Dekker, New York, pp 1–470
Kapikian AZ, Chanock RM (1990) Rotaviruses. In: Fields BN, Knipe DM (eds) Fundamental virology, 2nd ed. Raven Press, New York, pp 1353–1404
Konno T, Sato T, Suzuki H, Kitaoka S, Katsushima N, Sakamoto M, Yazaki N, Ishida N (1984) Changing RNA patterns in rotaviruses of human origin: demonstration of a single dominant pattern at the start of an epidemic and various patterns thereafter. J Infect Dis 149: 683–687
Laemmli U (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
Matsuno S, Hasegawa A, Mukoyama A, Inoye S (1985) A candidate for a new serotype of human rotavirus. J Virol 54: 623–624
Maunula L, Svensson L, von Bonsdorff C-H (1992) A family outbreak of gastroenteritis caused by group C rotavirus. Arch Virol 124: 269–278
Nakagomi O, Ohshima A, Aboudy Y, Shif I, Mochizuki M, Nakagomi T, Gotlieb ST (1990) Molecular identification by RNA-RNA hybridization of a human rotavirus that is closely related to rotaviruses of feline and canine origin. J Clin Microbiol 28: 1198–1203
Nakagomi T, Akatani K, Ikegami N, Katsushima N, Nakagomi O (1988) Occurrence of changes in human rotavirus serotypes with concurrent changes in genomic RNA electropherotypes. J Clin Microbiol 26: 2586–2592
Nakagomi T, Nakagomi O (1989) RNA-RNA hybridization identifies a human rotavirus that is genetically related to feline rotavirus. J Virol 63: 1431–1434
Nakagomi T, Oshima A, Akatani K, Ikegami N, Katsushima N, Nakagomi O (1990) Isolation and molecular characterization of a serotype 9 human rotavirus strain. Microbiol Immunol 34: 77–82
Noel JS, Beards GM, Cubitt WD (1991) Epidemiological survey of human rotavirus serotypes and electropherotypes in young children admitted to two children's hospitals in Northeast London from 1984 to 1990. J Clin Microbiol 29: 2213–2219
Nuttall SD, Hum CP, Holmes IH, Dyall-Smith ML (1989) Sequences of VP9 genes from short and supershort rotavirus strains. Virology 171: 453–457
Padilla-Noriega L, Arias CF, Lopez S, Puerto F, Snodgrass DR, Taniguchi K, Greenberg HB (1990) Diversity of rotavirus serotypes in Mexican infants with gastroenteritis. J Clin Microbiol 28: 1114–1119
Pedley S, Bridger JC, Brown JF, McCrae MA (1983) Molecular characterization of rotaviruses with distinct group antigens. J Gen Virol 64: 2093–2101
Pipittajan P, Kasempimolporn S, Ikegami N, Akatani K, Wasi C, Sinarachatanant P (1991) Molecular epidemiology of rotaviruses associated with pediatric diarrhoea in Bangkok, Thailand. J Clin Microbiol 29: 617–624
Rodger SM, Bishop RF, Birch C, McLean B, Holmes IH (1981) Molecular epidemiology of human rotaviruses in Melbourne, Australia, from 1973 to 1979, as determined by electrophoresis of genome ribonucleic acid. J Clin Microbiol 13: 272–278
Rodger SM, Holmes IH (1979) Comparison of the genomes of simian, bovine and human rotaviruses by gel electrophoresis and detection of genomic variation among bovine isolates. J Virol 30: 839–846
Svensson L, Uhnoo I, Grandien M, Wadell G (1986) Molecular epidemiology of rotavirus infections in Uppsala, Sweden, 1981: disappearance of a predominant electropherotype. J Med Virol 18: 101–111
Taniguchi K, Nishikawa K, Urasawa T, Urasawa S, Midthun K, Kapikian AZ, Gorziglia M (1989) Complete nucleotide sequence of the gene encoding VP4 of a human rotavirus (strain K8) which has unique VP4 neutralization epitopes. J Virol 63: 4101–4106
Taniguchi K, Urasawa T, Kobayashi N, Gorziglia M, Urasawa S (1990) Nucleotide sequence of VP4 and VP7 genes of human rotaviruses with subgroup I specificity and long RNA pattern: implication for new G serotype specificity. J Virol 64: 5640–5644
Taniguchi K, Urasawa T, Urasawa S (1994) Species specificity and interspecies relatedness in VP4 genotypes demonstrated by VP4 sequence analysis of equine, feline, and canine rotavirus strains. Virology 200: 390–400
Tufvesson (1983) Detection of a human rotavirus strain different from types 1 and 2 — A new subgroup? Epidemiology of subgroup in a swedish and ethiopian community. J Med Virol 12: 111–117
Unicomb LE, Bingnan F, Rahim Z, Banu NN, Gomes JG, Podder G, Munshi MH, Tzipori SR (1993) A one-year survey of rotavirus strains from three locations in Bangladesh. Arch Virol 132: 201–208
Unicomb LE, Bishop RF (1989) Epidemiology of rotavirus strains infecting children-throughout Australia during 1986–87. Arch Virol 106: 23–34
Urasawa S, Urasawa T, Taniguchi K, Wakasugi F, Kobayashi N, Chiba S, Sakurada N, Morita M, Morita O, Tokieda M, Kawamoto H, Minekawa Y, Ohseto M (1989) Survey of human rotavirus serotypes in different locales in Japan by enzyme-linked immunosorbent assay with monoclonal antibodies. J Infect Dis 160: 44–51
Vesikari T (1993) Clinical trials of live oral rotavirus vaccines: the Finnish experience. Vaccine 11: 255–261
Vesikari T, Isolauri E, Delem A, d'Hondt E, Andre FE, Beards GM, Flewett TH (1985) Clinical efficacy of the RIT 4237 live attenuated bovine rotavirus vaccine in infants vaccinated before a rotavirus epidemic. J Pediatr 107: 189–194
von Bonsdorff C-H, Svensson L (1988) Human serogroup C rotavirus in Finland. Scand J Infect Dis 20: 475–478
Woods PA, Gentsch J, Gouvea V, Mata L, Simhon A, Santosham M, Bai Z, Urasawa S, Glass RI (1992) Distribution of serotypes of human rotavirus in different populations. J Clin Microbiol 30: 781–785
Wyatt RG, James HJ, Pittman AL, Hoshino Y, Greenberg HB, Kalica AR, Flores J, Kapikian AZ (1983) Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes. J Clin Microbiol 18: 310–317
Zissis G, Lambert JP, Kapsenber JG, Enders G, Matanda LM (1981) Human rotavirus serotypes. Lancet i: 944–945
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Maunula, L., v. Bonsdorff, C.H. Rotavirus serotypes and electropherotypes in Finland from 1986 to 1990. Archives of Virology 140, 877–890 (1995). https://doi.org/10.1007/BF01314964
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DOI: https://doi.org/10.1007/BF01314964