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Antigens of infectious laryngotracheitis herpesvirus defined by monoclonal antibodies

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Summary

Monoclonal antibodies to glycoprotein and protein antigens of infectious laryngotracheitis virus (ILTV) were divided into five groups on the basis of their reactivity in immunofluorescence and Western blotting. Group I antibodies recognised a single band of 60 k and Group II antibodies recognised bands of 205, 160, 115, 90 and 85 k in Western blotting. In immunofluorescence both these groups of antibodies reacted with antigens located in the cytoplasm of fixed virus-infected cells and they also reacted with unfixed cells, suggesting that these antigens are on the surface of virus-infected cells. While Group I monoclonal antibodies did not react with extracts of tunicamycin-treated cells, some Group II antibodies recognised bands of decreased molecular weight compared to those present in untreated cells. The reactivity of the Group II antibodies with extracts of tunicamycin-treated cells suggested that they recognised at least three different epitopes which was confirmed by ELISA additivity assays. Monoclonal antibodies of Group III, Group IV and Group V recognised several low molecular weight proteins from 45 to 24 k. Immunofluorescence studies showed that these were nuclear and cytoplasmic antigens that were not present on the surface of virus-infected cells.

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Author notes

  1. S. Sonza

    Present address: Macfarlane Burnet Centre for Medical Research, Fairfield Hospital, Fairfield, Victoria, Australia

Authors and Affiliations

  1. Division of Animal Health, Animal Health Research Laboratory, CSIRO, Parkville

    Jennifer J. York, S. Sonza & K. J. Fahey

  2. Department of Veterinary Preclinical Sciences, University of Melbourne, Parkville, Victoria, Australia

    M. R. Brandon

Authors
  1. Jennifer J. York
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  2. S. Sonza
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  3. M. R. Brandon
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  4. K. J. Fahey
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York, J.J., Sonza, S., Brandon, M.R. et al. Antigens of infectious laryngotracheitis herpesvirus defined by monoclonal antibodies. Archives of Virology 115, 147–162 (1990). https://doi.org/10.1007/BF01310527

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  • DOI: https://doi.org/10.1007/BF01310527

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