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Defective transport of hemagglutinin-neuraminidase glycoprotein of bovine parainfluenza-3 virus in interferon treated cell

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Summary

A defective transport of bovine parainfluenza-3 virus (PI-3V) hemagglutinin-neuraminidase (HN) glycoprotein was evidenced in interferon (IFN)-treated bovine turbinate (BTu) cells. Indirect immunofluorescence performed with monoclonal antibody to PI-3 HN glycoprotein demonstrated accumulation of this protein in the perinuclear cytoplasm of IFN-treated cells. Untreated, infected control cells had a generalized widespread fluorescence. Unfixed control cells showed a uniform surface fluorescence in contrast to a few specs of fluorescence on the plasma membrane of IFN-treated cells. Electron microscopic localization of HN protein was done by immuno-gold ultrastructural cytochemistry. Untreated cells had uniform gold label on the plasma membrane and around the budding virus particles with no label in the cytoplasm. In IFN-treated cells, however, there was an accumulation of gold particles in the cytoplasm with only a few particles on the cell surface. Quantitative analysis of HN protein on the cell surface by solid phase radioimmune-assay revealed a greater amount of this protein on the surface of control cells, than those on the IFN-treated cells.

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Authors and Affiliations

  1. Virginia-Maryland Regional College of Veterinary Medicine, The University of Maryland, College Park, Maryland, U.S.A.

    Pinaki Panigrahi & Sashi B. Mohanty

  2. Department of Pathology, University of Maryland Medical School, 10 South Pine Street, M.S.T.F. Room 300, 21201, Baltimore, MD, U.S.A.

    Pinaki Panigrahi

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  1. Pinaki Panigrahi
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  2. Sashi B. Mohanty
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Panigrahi, P., Mohanty, S.B. Defective transport of hemagglutinin-neuraminidase glycoprotein of bovine parainfluenza-3 virus in interferon treated cell. Archives of Virology 109, 125–133 (1989). https://doi.org/10.1007/BF01310524

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  • DOI: https://doi.org/10.1007/BF01310524

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