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Some biological properties of a rhabdovirus isolated from penaeid shrimps

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Summary

Some of the relevant biological properties of a rhabdovirus isolated from penaeid shrimps (RPS) were examined. The virus replicated in an established fish cell line, epithelioma papulosum cyprini (EPC) which allowed for the development of a quantitative plaque assay protocol. Virus replication was not inhibited by the DNA antagonist, 5-bromo-2′-deoxyuridine (20 µg/ml). Virus infectivity was sensitive to 20% ethyl ether, low pH, and to 37 °C. The virus showed marked lability to repeated freezing and thawing and storage at −10°C, but was stable at −70°C for several weeks. The virus particle to infectious unit ratio in EPC was found to be 30.

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References

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Authors and Affiliations

  1. Department of Microbiology, University of Hawaii, Honolulu, Hawaii, USA

    Yuanan Lu & P. C. Loh

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  1. Yuanan Lu
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  2. P. C. Loh
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Lu, Y., Loh, P.C. Some biological properties of a rhabdovirus isolated from penaeid shrimps. Archives of Virology 127, 339–343 (1992). https://doi.org/10.1007/BF01309596

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  • DOI: https://doi.org/10.1007/BF01309596

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