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Immunological study of a cellular 35K phosphorylated polypeptide detected in Rous sarcoma virus transformed cells

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Summary

We have immunoprecipitated a phosphoprotein of 35K daltons (35K) common to RSV-transformed chick embryo fibroblasts (CEF) and rodent cells. The phosphorylation of this antigen depends on the expression of thev-src gene and contains phosphotyrosine. The pre-existing 35K protein, of CEF infectedde novo, was further shown to become phosphorylated shortly after the appearance of active pp60s-src, and about 1 day before morphological transformation. The experiments with RSV-transformed rodent cells have shown that the 35K phosphoprotein is associated with the cellular framework. Another phosphoprotein of 37K was found in the RSV-transformed rodent cells, but not in the transformed CEF. However, this protein was not phosphorylated at tyrosine residues and its phosphorylation persisted, at the restrictive temperature, in cells transformed by ats mutant of RSV.

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Authors and Affiliations

  1. Unité de Virologie Fondamentale et Appliquée, INSERM, Groupe de Recherche 33, CNRS, Lyon, France

    S. Y. Lee, J. Paire, G. Vernet & V. Krsmanovic

  2. Groupe de Recherche 43, CNRS, associé à l'Institut Curie (Institut du Radium), Faculté des Sciences, Orsay, France

    J. M. Biquard

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  1. S. Y. Lee
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Lee, S.Y., Paire, J., Vernet, G. et al. Immunological study of a cellular 35K phosphorylated polypeptide detected in Rous sarcoma virus transformed cells. Archives of Virology 77, 195–208 (1983). https://doi.org/10.1007/BF01309267

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  • DOI: https://doi.org/10.1007/BF01309267

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