Abstract
We investigated whether the recently described endothelium-derived nitric oxide-mediated gastric hyperemia in the uremic rat protects the gastric mucosa against ethanol injury. Uremia was induced by subtotal nephrectomy. Basal gastric mucosal blood flow, measured by a hydrogen gas clearance technique, was significantly higher in uremic than control rats. Continuous intragastric perfusion with 40% ethanol produced significantly less gross and histological lesions in uremic than in control rats. The administration of 3 mg/kg ofN W-nitro-l-arginine methyl ester, a specific inhibitor of nitric oxide biosynthesis, decreased resting gastric mucosal blood flow to control levels in uremic rats, but had no effect on basal gastric blood flow in control rats. This pretreatment with the inhibitor of nitric oxide biosynthesis increased 40% ethanol-induced gastric mucosal lesions in uremic rats to the same level as that observed in control rats, but had no effect on lesions in control rats. In conclusion, this study suggests that in the uremic rat, gastric hyperemia, mediated by increased endothelium-derived nitric oxide, attenuates ethanol-induced gastric mucosal injury.
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Dr. Enrique Quintero is the recipient of a Fogarty International Fellowship Award (N.I.H.) (1 FO5 TW04443-01) and a Grant from Consejería de Educación, Cultura y Deportes of the Canary Islands Autonomous Government. This work was also supported by Veterans Administration Research Funds.
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Quintero, E., Guth, P.H. Nitric oxide-mediated gastric hyperemia decreases ethanol-induced gastric mucosal injury in uremic rats. Digest Dis Sci 37, 1324–1328 (1992). https://doi.org/10.1007/BF01295999
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DOI: https://doi.org/10.1007/BF01295999