Abstract
Purification of Phosphatidylinositol (PI) kinase was attempted from bovine brain. A seven step purification protocol increased the specific activity 100×but attempts at further purification were unsuccessful. Labeling of the partially purified PI kinase with the ATP analog fluorosulfonylbenzoyl adenosine reproducibly identified three bands on polyacrylamide gel electrophoresis of 76 K, 45 K, and 29 K, one of which likely represents PI kinase. Kinetic studies showed aK m of 17 μM for ATP, 0.02 mg/ml for PI and aV m of 1830 pmol/min/mg protein for ATP and 820 pmol/min/mg protein for PI.
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Thompson, D.M., Verma, B. & Thomas, C. Partial purification and charcterization of phosphatidylinositol kinase from bovine brain. Neurochem Res 13, 417–421 (1988). https://doi.org/10.1007/BF01268875
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DOI: https://doi.org/10.1007/BF01268875