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Studies on the control of myelinogenesis. I. Myelination of regenerating axons after entry into a foreign unmyelinated nerve

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Journal of Neurocytology

Summary

The proximal stump of a predominantly myelinated nerve (sternohyoid) was anastomosed in a tube to the distal stump of a largely unmyelinated nerve (cervical sympathetic) in order to explore the role of the axon in activating Schwann cells to produce myelin. The two nerves were examined after being united for periods between 3 and 15 weeks. At these times, regenerated myelinated and nonmyelinated fibres, originating from the proximal stump, were seen within the original fascicle in the distal stump of the sympathetic trunk. There was a significant (p < 0.01) increase in the mean number of myelinated fibres in the anastomosed sympathetic nerves from the mean number of myelinated fibres in the normal sympathetic nerve. The histological and ultrastructural features of the anastomosed nerves are described and the differences between the morphology of the intratubal and extratubal regions are highlighted. The results of this study may indicate that the axon instructs the Schwann cell to produce myelin, but before this conclusion is reached, the origin of the myelinating cells has to be determined.

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References

  • Abercrombie, M., Evans, D. H. L. andMurray, J. G. (1959) Nuclear multiplication and cell migration in degenerating unmyelinated nerve fibres.Journal of Anatomy (London) 93, 9–14.

    Google Scholar 

  • Abercrombie, M., Johnson, M. L. andThomas, G. A. (1949) The influence of nerve fibres on Schwann cell migration in tissue culture.Proceedings of the Royal Society of London, Series B 136, 448–60.

    Google Scholar 

  • Aguayo, A. (1974) Personal communication.

  • Aguayo, A. J., Martin, J. B. andBray, G. M. (1972) Effects of nerve growth factor antiserum on peripheral unmyelinated nerve fibers.Acta Neuropathologica 20, 288–98.

    Google Scholar 

  • Aguayo, A. J., Nair, C. P. andMidgley, R. (1971) Experimental compression neuropathy in the rabbit. Histologic and electrophysiological studies.Archives of Neurology 24, 358–64.

    Google Scholar 

  • Aitken, J. T. (1949) The effect of peripheral connexions on the maturation of regenerating nerve fibres.Journal of Anatomy (London) 83, 32–43.

    Google Scholar 

  • Aitken, J. T., Sharman, M. andYoung, J. Z. (1947) Maturation of regenerating nerve fibres with various peripheral connexions.Journal of Anatomy (London) 81, 1–22.

    Google Scholar 

  • Asbury, A. K. (1975) The Biology of Schwann Cells. InPeripheral Neuropathy (edited byDyck, P. J., Thomas, P. K. andLambert, E. H.), in press. Philadelphia: Saunders.

    Google Scholar 

  • Brady, R. O. andQuarles, R. H. (1973) The enzymology of myelination.Molecular and Cellular Biochemistry 2, 23–9.

    Google Scholar 

  • Bray, G. M., Aguayo, A. andMartin, J. B. (1973) Immunosympathectomy: late effects on the composition of rat cervical sympathetic trunks and influence on axonal regeneration after crush.Acta Neuropathologica 26, 345–52.

    Google Scholar 

  • Bray, G. M. andAguayo, A. J. (1974) Regeneration of peripheral unmyelinated nerves. Fate of the axonal sprouts which develop after injury.Journal of Anatomy (London) 117, 517–29.

    Google Scholar 

  • Bunge, R. P. (1968) Glial cells and the central myelin sheath.Physiological Reviews 48, 197–251.

    Google Scholar 

  • Cajal, S. R. Y. (1928)Degeneration and Regeneration of the Nervous System (translated and edited byMay, R. M.), volume 1, London and New York: Hafner Publ. Co.

    Google Scholar 

  • Davison, A. N. andPeters, A. (1970)Myelination. Springfield, Illinois: Charles C. Thomas.

    Google Scholar 

  • Denny-Brown, D. (1946) Importance of neural fibroblasts in the regeneration of nerve.Archives of Neurology and Psychiatry (Chicago) 55, 171–215.

    Google Scholar 

  • Duncan, D. (1934) A relation between axon diameter and myelination determined by measurements of myelinated spinal root fibres.Journal of Comparative Neurology 60, 437–71.

    Google Scholar 

  • Duncan, D. (1948) Alterations in the structures of nerves caused by restricting their growth by ligatures.Journal of Neuropathology and Experimental Neurology 7, 261–73.

    Google Scholar 

  • Dyck, P. J. andHopkins, A. P. (1972) Electron microscopic observations on degeneration and regeneration of unmyelinated fibres.Brain 95, 223–34.

    Google Scholar 

  • Edds, M. V. Jr (1953) Collateral nerve regeneration.Quarterly Review of Biology 28, 260–76.

    Google Scholar 

  • Evans, D. H. L. andMurray, J. G. (1954) Regeneration of non-medullated nerve fibres.Journal of Anatomy (London) 88, 465–80.

    Google Scholar 

  • Evans, D. H. L. andMurray, J. G. (1956) A study of regeneration in a motor nerve with a unimodal fibre diameter distribution.Anatomical Record 126, 311–33.

    Google Scholar 

  • Foley, J. O. (1943) Composition of the cervical sympathetic trunk.Proceedings of the Society for Experimental Biology and Medicine 52, 212–4.

    Google Scholar 

  • Foley, J. O. andDubois, F. S. (1940) A quantitative and experimental study of the cervical sympathetic trunk.Journal of Comparative Neurology 72, 587–603.

    Google Scholar 

  • Fraher, J. P. (1972) A quantitative study of anterior root fibres during early myelination.Journal of Anatomy (London) 112, 99–129.

    Google Scholar 

  • Friede, R. L. (1972) Control of myelin formation by axon caliber (with a model of the control mechanism).Journal of Comparative Neurology 144, 233–52.

    Google Scholar 

  • Friede, R. L. andSamorajski, T. (1968) Myelin formation in the sciatic nerve of the rat. A quantitative electron microscope, histochemical and radioautographic study.Journal of Neuropathology and Experimental Neurology 27, 546–71.

    Google Scholar 

  • Greene, E. C. (1968)Anatomy of the Rat. New York and London: Hafner Publ. Co.

    Google Scholar 

  • Hillarp, N. Å. andOlivecrona, H. (1946) The rôle played by the axon and the Schwann cells in the degree of myelination of the peripheral nerve fibre.Acta Anatomica 2, 17–32.

    Google Scholar 

  • Holmes, W. andYoung, J. Z. (1942) Nerve regeneration after immediate and delayed suture.Journal of Anatomy (London) 77, 63–96.

    Google Scholar 

  • Joseph, J. andWendell-Smith, C. P. (1959) Nuclear population changes in degenerating nonmyelinated nerves.Journal of Anatomy (London) 93, 460–4.

    Google Scholar 

  • King, R. H. M. andThomas, P. K. (1971) Electron microscope observations on aberrant regeneration of unmyelinated axons in the vagus nerve of the rabbit.Acta Neuropathologica 18, 150–9.

    Google Scholar 

  • Langley, J. N. (1898) On the union of cranial autonomic (visceral) fibres with the nerve cells of the superior cervical ganglion.Journal of Physiology (London) 32, 240–70.

    Google Scholar 

  • Langley, J. N. andAnderson, H. K. (1903) On the union of the fifth cervical nerve with the superior cervical ganglion.Journal of Physiology (London) 30, 439–42.

    Google Scholar 

  • Langley, J. N. andAnderson, H. K. (1904) The union of different kinds of nerve fibres.Journal of Physiology (London) 31, 365–91.

    Google Scholar 

  • Lubińska, L. (1961) Sedentary and migratory states of Schwann cells.Experimental Cell Research 8, 74–90.

    Google Scholar 

  • Martinez, A. J. andFriede, R. L. (1970) Changes in nerve cell bodies during myelination of their axons.Journal of Comparative Neurology 138, 329–38.

    Google Scholar 

  • Matthews, M. A. (1968) An electron microscopic study of the relationship between axon diameter and the initiation of myelin production in the peripheral nervous system.Anatomical Record 161, 337–51.

    Google Scholar 

  • Mokrasch, L. C., Bear, R. S. andSchmitt, F. O. (1971) Myelin. A report based on an NRP work session.Neurosciences Research Program Bulletin 9 No. 4.

  • Morris, J. H., Hudson, A. R. andWeddell, G. (1972) A study of degeneration and regeneration in the divided rat sciatic nerve based on electron microscopy. IV. Changes in fascicular microtopography, perineurium and endoneurial fibroblasts.Zeitschrift für Zellforschung und mikroskopische Anatomie 124, 165–203.

    Google Scholar 

  • Ochoa, J. (1971) The sural nerve of the human foetus: electron microscope observations and counts of axons.Journal of Anatomy (London) 108, 231–45.

    Google Scholar 

  • Ochoa, J. andMair, W. G. P. (1969) The normal sural nerve in man. II. Changes in the axons and Schwann cells due to ageing.Acta Neuropathologica 13, 217–39.

    Google Scholar 

  • Ohmi, S. (1961) Electron microscopic study of Wallerian degeneration in the peripheral nerves.Zeitschrift für Zettforschung und mikroskopische Anatomie 54, 39–67.

    Google Scholar 

  • Peters, A., Palay, S. L. andWebster, H. Def. (1970)The Fine Structure of the Nervous System. The Cells and their Processes. New York: Hoeber, Harper and Row.

    Google Scholar 

  • Peterson, E. R. andMurray, M. R. (1955) Myelin sheath formation in cultures of avian spinal ganglia.American Journal of Anatomy 96, 319–55.

    Google Scholar 

  • Ranson, S. W. (1912) Degeneration and regeneration of nerve fibres.Journal of Comparative Neurology 22, 487–545.

    Google Scholar 

  • Sanders, F. K. andYoung, J. Z. (1946) The influence of peripheral connexion on the diameter of regenerating nerve fibres.Journal of Experimental Biology 22, 203–12.

    Google Scholar 

  • Schröder, J. M. (1970) Feinstruktur und quantitativen Auswertung regenerierter peripherer Nervenfasern. InProceedings of the VIth International Congress of Neuropathology (edited byMasson Et Cie), pp. 628–46. Paris.

  • Shawe, G. D. H. (1955) On the number of branches formed by regenerating nerve fibres.British Journal of Surgery 42, 474–88.

    Google Scholar 

  • Simpson, S. A. andYoung, J. Z. (1945) Regeneration of fibre diameter after cross-unions of visceral and somatic nerves.Journal of Anatomy (London) 79, 48–65.

    Google Scholar 

  • Singer, M. (1968) Penetration of labelled amino acids into the peripheral nerve fibre from surrounding body fluids. InCiba Foundation Symposium, Growth of the Nervous System (edited byWolsten-Holme, G. E. andO'Connor, M.) pp. 200–15.

  • Singer, M. andSteinberg, M. C. (1972) Wallerian degeneration: A reevaluation based on transected and colchicine-poisoned nerves in the amphibianTriturus.American Journal of Anatomy 133, 51–84.

    Google Scholar 

  • Speidel, C. C. (1964)In vivo studies of myelinated nerve fibres.International Review of Cytology 16, 173–231.

    Google Scholar 

  • Spencer, P. S. (1971)Light and Electron Microscopic Observations on Localised Peripheral Nerve Injuries. Ph.D. thesis. University of London.

  • Spencer, P. S. (1972) Reappraisal of the model for ‘bulk axoplasmic flow’.Nature New Biology 240, 283–5.

    Google Scholar 

  • Spencer, P. S. (1974) The traumatic neuroma and proximal stump.Bulletin of the Hospital for Joint Disease,35, 85–102.

    Google Scholar 

  • Spencer, P. S., Raine, C. S. andWiśniewski, H. (1973) Axon diameter and myelin thickness — unusual relationships in dorsal root ganglia.Anatomical Record 176, 225–44.

    Google Scholar 

  • Spencer, P. S. andThomas, P. K. (1970) The examination of isolated nerve fibres by light and electron microscopy, with observations on demyelination proximal to neuromas.Acta Neuropathologica 16, 177–86.

    Google Scholar 

  • Thomas, G. A. (1948) Quantitative histology of Wallerian degeneration. II Nuclear population in two nerves of different fibre spectrum.Journal of Anatomy (London) 82, 135–45.

    Google Scholar 

  • Thomas, P. K. (1964) Changes in the endoneurial sheaths of peripheral myelinated nerve fibres during Wallerian degeneration.Journal of Anatomy (London) 98, 175–82.

    Google Scholar 

  • Thomas, P. K. (1971) The morphological basis for alterations in nerve conduction in peripheral neuropathy.Proceedings of the Royal Society of Medicine (London) 64, 295–8.

    Google Scholar 

  • Thomas, P. K. (1974) Nerve Injury. InEssays on the Nervous System. A Festschrift for Professor J. Z. Young (edited byBellairs, R. andGray, E. G.) pp. 44–70. Oxford: Clarendon Press.

    Google Scholar 

  • Thomas, P. K. andJones, D. G. (1967) The cellular response to nerve injury. 2. Regeneration of the perineurium after nerve section.Journal of Anatomy (London) 101, 45–55.

    Google Scholar 

  • Webster, H. Def., Martin, J. R. andO'Connell, M. F. (1973) The relationships between interphase Schwann cells and axons before myelination: A quantitative electron microscopic study.Developmental Biology 32, 401–16.

    Google Scholar 

  • Weiss, P. A. andHiscoe, H. B. (1948) Experiments on the mechanism of nerve growth.Journal of Experimental Zoology 107, 315–95.

    Google Scholar 

  • Williams, P. L. andWendell-Smith, C. P. (1971) Some additional parametric variations between peripheral nerve fibre populations.Journal of Anatomy (London) 109, 505–26.

    Google Scholar 

  • Young, J. Z. (1945) History of the Shape of a Nerve Fibre. InEssays on Growth and Form, Presented to D'Arcy W. Thompson, (edited byLe Gros Clarke, W. E. andMedawar, P. B.) pp. 41–94. Oxford: Clarendon Press.

    Google Scholar 

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Authors and Affiliations

  1. Department of Pathology (Neuropathology), and the Rose F. Kennedy Center for Research in Mental Retardation and Human Development, Albert Einstein College of Medicine, Bronx, New York

    Harold J. Weinberg & Peter S. Spencer

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  1. Harold J. Weinberg
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  2. Peter S. Spencer
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Weinberg, H.J., Spencer, P.S. Studies on the control of myelinogenesis. I. Myelination of regenerating axons after entry into a foreign unmyelinated nerve. J Neurocytol 4, 395–418 (1975). https://doi.org/10.1007/BF01261372

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  • DOI: https://doi.org/10.1007/BF01261372

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