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Studies on inhibition of viral oncogenesis

III. Effect of clam extracts and methotrexate on tumor formation in male and female hamsters induced by virulent and attenuated Adenovirus-12

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Summary

Newborn Syrian hamsters (Mesocricatus auratus) were inoculated subcutaneously at birth with adenovirus-12, Hollinshead strain, and 4–5 days later they were treated with clam (M. mercenaria) liver extracts (CLE). The animals were segregated according to sex at 3 weeks of age. The tumor incidence in the males, both treated and controls, was statistically lower than in the corresponding females. The treated hamsters, both males and females, developed significantly fewer tumors than the corresponding controls. During the course of study the virus lost much of its oncogenicity but all animals inoculated with either the virulent or attenuated virus responded to treatments. The CLE in a total dose of 100–350 mcg per hamster given in 2–3 subcutaneous injections within 12 days after birth was effective in protecting some of the hamsters from developing tumors during the later part of their life. The common antitumor drug, methotrexate (MTX), also showed some inhibitory activity, at least in the male hamsters. The over-all results of 11 assay experiments, using 663 hamsters, carried out in our laboratory during the past several years showed that treatment with clam extracts reduced the tumor incidence by 50% or more as compared to the controls.

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Authors and Affiliations

  1. Department of Health, Education, and Welfare, Public Health Service, National Institutes of Health, USA

    C. P. Li, N. M. Tauraso, B. Eddy, B. Prescott & E. C. Martino

  2. Division of Biologics Standards, Laboratory of Virology and Rickettsiology, Bethesda, Maryland, USA

    C. P. Li, N. M. Tauraso, B. Eddy, B. Prescott & E. C. Martino

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  1. C. P. Li
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  2. N. M. Tauraso
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  3. B. Eddy
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  4. B. Prescott
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  5. E. C. Martino
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Li, C.P., Tauraso, N.M., Eddy, B. et al. Studies on inhibition of viral oncogenesis. Archiv f Virusforschung 36, 284–295 (1972). https://doi.org/10.1007/BF01249859

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