Summary
Bovine pineal glands were extracted according to the methods reported byBensinger et al. (1973) andCheesman andFariss (1970). Isobutanol soluble COH-inhibiting activity was further separated by chromatography on Sephadex G-15 and paper chromatography.
With the Bensinger method, different active pineal fractions were obtained from Sephadex G-15 columns. Certain of those fractions were further separated by paper chromatography in butanol: acetic acid: water (4∶1∶1) and the COH-inhibitor was localized. The pineal COH-inhibitor could also be localized by high pressure, reverse phase liquid chromatography. More COH-inhibiting activity was extracted with the Bensinger method than with aqueous and acetic acid extraction methods used earlier by us. The Cheesman extraction method for arginine vasotocin gave less regular results in our hands than the Bensinger extraction method.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aures, D., Fleming, R., Håkanson, R. Separation and detection of biogenic amines by thin layer chromatography. Microanalysis of tissue amines and of enzymes involved in their metabolism. J. of Chrom.33, 480–493 (1968).
Bensinger, R., Vaughan, M., Klein, D. C. Isolation of a non-melatonin lipophilic antigonadotropic factor from the bovine pineal gland. Fed. Proc.32, 282 (1973).
Benson, B., Matthews, M. J., Rodin, A. E. Studies on a non-melatonin pineal antigonadotropin. Acta Endocrinol.69, 257–266 (1972).
Blask, D. E., Vaughan, M. K., Reiter, R. J., Johnson, L. Y., Vaughan, G. M. Prolactin-releasing and release-inhibiting factor activities in the bovine, rat and human pineal gland.In vitro andin vivo studies. Endocrinology99, 152–162 (1976).
Cheesman, D. W. Structural elucidation of a gonadotropin-inhibiting substance from the bovine pineal gland. Biochim. Biophys. Acta207, 247–253 (1970).
Cheesman, D. W., Fariss, B. Isolation and characterization of a gonadotropin-inhibiting substance from the bovine pineal gland. Proc. Soc. Exp. Biol. Med.133, 1254–1256 (1970).
Ebels, I., Benson, B., Matthews, M. J. Localization of a sheep pineal antigonadotropin. Anal. Biochem.56, 546–565 (1973).
Ebels, I., Benson, B., Bria, C. F., McDonnell, D., Chang, S. Y., Hruby, V. J. Location by paper chromatography of compensatory ovarian hypertrophy (COH) inhibiting activity in acetic acid extracts from bovine pineals. J. Neural Transm.42, 275–292 (1978).
Rosenblum, I. Y., Benson, B., Bria, C. F., McDonnell, D., Hruby, V. J.: Localization and chemical characterization of a partially purified bovine pineal antigonadotropin (in press).
Author information
Authors and Affiliations
Additional information
This work was supported by NIH Grant no. HD 08759.
Rights and permissions
About this article
Cite this article
Ebels, I., Benson, B., Bria, C.F. et al. Location by paper chromatography of compensatory ovarian hypertrophy (COH) inhibiting activity in isobutanol extracts of bovine pineals. J. Neural Transmission 45, 43–61 (1979). https://doi.org/10.1007/BF01243880
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01243880