Summary
A system for detecting Equine infections anemia viral antigen by direct immunofluorescence and the characteristics of the fluorescent antigen in horse peripheral leukocyte cultures are described. Serum from chronically infected horses yielded conjugates which gave bright specific staining of EIA antigen. The antigen was present by the second day after inoculation of the cultures. It appeared as discrete cytoplasmic dots, irregular cytoplasmic masses and accumulations along the cell membrane. The percentage of infected cells increased from 10–15% on the second day to 80% on the fifth day after inoculation.
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Crawford, T.B., McGuire, T.C. & Henson, J.B. Detection of Equine infectious anemia virusin vitro by immunofluorescence. Archiv f Virusforschung 34, 332–339 (1971). https://doi.org/10.1007/BF01242979
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DOI: https://doi.org/10.1007/BF01242979